Difference between revisions of "Team:Potsdam/Parts"

 
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     <div class="wrapper style3">
 
     <div class="wrapper style3">
 
         <div class="title">
 
         <div class="title">
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             Project design
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             Parts
 
         </div>
 
         </div>
 
         <div id="main" class="container">
 
         <div id="main" class="container">
 
             <div id="content">
 
             <div id="content">
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<p>
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  <img src="https://static.igem.org/mediawiki/2017/f/fc/T--Potsdam--parts--puzzle.png">
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<h5>What should this page contain?</h5>
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<ul>
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<li>Explanation of the engineering principles your team used in your design</li>
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<li>Discussion of the design iterations your team went through</li>
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<li>Experimental plan to test your designs</li>
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</ul>
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</p>
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            </div>
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        </div>
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    </div>
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<div align="justify">
  
  
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</div> Due to time constraints, we were only able to send in the parts:
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483000">BBa_K2483000</a> (IAA-RBP fusions),
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483005">BBa_K2483005</a> (Target cassette with 6 bp spacer),
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483006">BBa_K2483006</a> (Target cassette with 18 bp spacer) and
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483007">BBa_K2483007</a> (Ddx4-YFP).
 +
<br> The rest of the parts:
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483002">BBa_K2483002</a> (LacI-dCas9),
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483004">BBa_K2483004</a> (final low-copy construct),
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483008">BBa_K2483008</a> (Ddx4-IAAs),
 +
<br> <a href="http://parts.igem.org/Part:BBa_K2483009">BBa_K2483009</a> (yeast optimized IAAM and IAAH)
 +
<br> is planned but not sent to the reigstry with 007-009 belonging to the LLPS project and 000-006 to dCas9. Please remember that we forgot to check for illegal restriction sites in the parts 008 and 009 which resulted in a SpeI site in both of them.
 +
            </div>
 
         </div>
 
         </div>
 
     </div>
 
     </div>
  
  
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    <div id="footer-wrapper" class="wrapper">
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      <div id="footer-wrapper" class="wrapper">
 
         <div class="title">
 
         <div class="title">
 
             Did you see this?
 
             Did you see this?
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                 <div class="topBox">
 
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                     <a href="http://www.google.com/">
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                     <a href="https://2017.igem.org/Http://2017.igem.org/2017.igem.org/Team:Potsdam/Abstract">
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                         <img src="https://static.igem.org/mediawiki/2017/f/fc/T--Potsdam--pic01.jpg" alt="" />  
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                         <img src="https://static.igem.org/mediawiki/2017/archive/0/01/20171003140936%21T--Potsdam--Abstract--MC-Drawing.jpeg" alt="" />  
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                         <p>First cool link page</p>
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                         <p><font color="black">Abstract</font></p>
 
                     </a>
 
                     </a>
 
                 </div>
 
                 </div>
 
                 <div class="bottomBox">
 
                 <div class="bottomBox">
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                     <a href="http://www.google.com/">
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                     <a href="https://2017.igem.org/wiki/index.php?title=Team%3APotsdam%2FResults"">
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                         <img src="https://static.igem.org/mediawiki/2017/7/74/T--Potsdam--pic05.jpg" alt="" />  
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                         <img src="https://static.igem.org/mediawiki/2017/5/50/T--Potsdam--resuslts--main.png" alt="" />  
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                         <p> Anotha one</p>
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                         <p><font color="black">What we've gained</font> </p>
 
                     </a>
 
                     </a>
 
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                 </div>
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                     <a href="http://www.google.com/">
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                     <a href="https://2017.igem.org/Team:Potsdam/iGem-goes-green">
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                         <img src="https://static.igem.org/mediawiki/2017/4/47/T--Potsdam--pic06.jpg" alt="" />  
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                         <img src="https://static.igem.org/mediawiki/2017/1/12/T--Potsdam--goesgreen.png" alt="" />  
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                         <p>AND ANOTHA ONE</p>
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                         <p><font color="black">iGEM Goes Green</font></p>
 
                     </a>
 
                     </a>
 
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                 <div class="bottomBox">
 
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                     <a href="http://www.google.com/">
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                     <a href="https://2017.igem.org/wiki/index.php?title=Team%3APotsdam%2FTeam">
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                         <img src="https://static.igem.org/mediawiki/2017/d/da/T--Potsdam--pic07.jpg" alt="" />
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                         <img src="https://static.igem.org/mediawiki/2017/d/d5/T--Potsdam--schattenmenschen.png" alt="" />
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                         <p> Wow</p>
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                         <p> <font color="black"> Who we are...</font> </p>
 
                     </a>
 
                     </a>
 
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                 <div class="topBox">
 
                 <div class="topBox">
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                     <a href="http://www.google.com/">
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                     <a href="https://2017.igem.org/wiki/index.php?title=Team%3APotsdam%2FBackground">
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                         <img src="https://static.igem.org/mediawiki/2017/2/29/T--Potsdam--pic02.jpg" alt="" />  
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                         <img src="https://static.igem.org/mediawiki/2017/a/a6/T--Potsdam--infografik.png" alt="" />  
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                         <p>when will it end</p>
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                         <p><font color="black">Background information</font></p>
 
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                     </a>
 
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                 <div class="bottomBox">
 
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                     <a href="http://www.google.com/">
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                     <a href="https://2017.igem.org/wiki/index.php?title=Team%3APotsdam%2Fcrowdfunding">
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                         <img src="https://static.igem.org/mediawiki/2017/a/a7/T--Potsdam--pic03.jpg" alt="" />  
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                         <img src="https://static.igem.org/mediawiki/2017/a/a5/T--Potsdam--main--startnext.png" alt="" />  
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                         <p>it was a great adventure</p>
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                         <p><font color="black">Crowdfunding</font></p>
 
                     </a>
 
                     </a>
 
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</div>
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<div id="sponsor-wrapper" class="wrapper">
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<p> <font color="black"> Main sponsors: </font> </p>
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    <img src="https://static.igem.org/mediawiki/2017/f/fd/T--Potsdam--sponors--up_unten.png" width="24%">
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    <img src="https://static.igem.org/mediawiki/2017/8/8d/T--potsdam--sponsors--maxsynbio_unten.png" width="24%">
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    <img src="https://static.igem.org/mediawiki/2017/8/8f/T--Potsdam--sponsors--promega_unten.png" width="24%">
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    <img src="https://static.igem.org/mediawiki/2017/b/b2/T--Potsdam--sponsors--eppendorf.png" width="24%">
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</div>
 
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</div>
 
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</div>

Latest revision as of 22:44, 1 November 2017

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Parts
Due to time constraints, we were only able to send in the parts:
BBa_K2483000 (IAA-RBP fusions),
BBa_K2483005 (Target cassette with 6 bp spacer),
BBa_K2483006 (Target cassette with 18 bp spacer) and
BBa_K2483007 (Ddx4-YFP).
The rest of the parts:
BBa_K2483002 (LacI-dCas9),
BBa_K2483004 (final low-copy construct),
BBa_K2483008 (Ddx4-IAAs),
BBa_K2483009 (yeast optimized IAAM and IAAH)
is planned but not sent to the reigstry with 007-009 belonging to the LLPS project and 000-006 to dCas9. Please remember that we forgot to check for illegal restriction sites in the parts 008 and 009 which resulted in a SpeI site in both of them.