Difference between revisions of "Team:Exeter/Lab Conclusion"
Rahannazeer (Talk | contribs) |
|||
| Line 43: | Line 43: | ||
<h2 id="h1">Modified Pili Expression</h2> | <h2 id="h1">Modified Pili Expression</h2> | ||
| + | |||
| + | <p>Over the duration of the project we endeavored to answer a number of questions experimentally. Can we modify FimH with large proteins? Can the modified FimH be exported and can pilus biogenesis occur when large proteins have been fused? Can we modify the FimH protein at the N-terminal, after the signal peptide cleavage point? Can we drive expression of the <i>fim operon</i> with a constitutive promoter? </p> | ||
<h2 id="h2">Applied Design</h2> | <h2 id="h2">Applied Design</h2> | ||
Revision as of 23:13, 1 November 2017
Conclusion
Modified Pili Expression
Over the duration of the project we endeavored to answer a number of questions experimentally. Can we modify FimH with large proteins? Can the modified FimH be exported and can pilus biogenesis occur when large proteins have been fused? Can we modify the FimH protein at the N-terminal, after the signal peptide cleavage point? Can we drive expression of the fim operon with a constitutive promoter?
Applied Design
Hydrocyclone
Metal Binding Reactor
Biosecurity
The results we obtained showed that a 10 minute exposure to UV was not able to cause sufficient cell death and therefore is not suitable for use as a biosecurity mechanism in our filtration system. In order to achieve the industry required escape frequency, which is of the order 10-8, the water would have to be irradiated for a much more significant period of time which is not practical for our intended use. Therefore we need to explore possible alternatives, such copper alginate beads as detailed on the Applied Design page.
