Difference between revisions of "Team:IIT Delhi/Safety"

 
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                                                         <button class="dropbtn1"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button>
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                                                         <button class="dropbtn2"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button>
 
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       <a href="/Team:IIT_Delhi/Description">Description</a>
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       <a href="/Team:IIT_Delhi/Description">Overview</a>
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       <a href="/Team:IIT_Delhi/Results">Results</a>
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       <a href="/Team:IIT_Delhi/Design">Squarewave Generator</a>
  
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       <a href="/Team:IIT_Delhi/Interlab">Interlab</a>
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      <a href="/Team:IIT_Delhi/Photobleaching">Photobleaching</a>
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      <a href="/Team:IIT_Delhi/Promoter">Promoter strength</a>
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                                                      <a href="/Team:IIT_Delhi/Oscillations">Oscillations</a>
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       <a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a>
 
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       <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a>
 
       <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a>
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       <a href="/Team:IIT_Delhi/Model">Overview</a>
 
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       <a href="/Team:IIT_Delhi/Database">Simulation Database</a>
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       <a href="/Team:IIT_Delhi/Write_Model">Writing a Model</a>
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                                                        <a href="/Team:IIT_Delhi/Deterministic_Model">Deterministic Model </a>
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                                                        <a href="/Team:IIT_Delhi/Stochastic_Model">Stochastic Model</a>
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       <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a>
 
       <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a>
 
       <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a>
 
       <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a>
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                                                        <a href="/Team:IIT_Delhi/Safety">Safety</a>
 
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                                                        <a href="/Team:IIT_Delhi/Collaborations">Overview</a>
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      <a href="/Team:IIT_Delhi/GMM_legislation">GMM Legislation</a>
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      <a href="/Team:IIT_Delhi/berlin">iGEM Berlin</a>
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      <a href="/Team:IIT_Delhi/mohali">Mentoring IISER Mohali</a>
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      <a href="/Team:IIT_Delhi/glasgow">iGEM Glasgow</a>
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             <h2 class="h2font">SAFETY</h2>
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             <h2 class="h2font">Safety</h2>
  
 
             <p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--IIT_Delhi--Berlin.jpg" alt="" /></span><br> The IIT Delhi iGEM team whole-heartedly collaborated with Berlin team. Berlin team was working on the affordable, practical and effective diagnostic of the widespread third world parasitic disease -Tapeworm Infection. Since India is one of the countries where millions of people are infected by this parasite, IIT Delhi directed Berlin team to a suitable site. It was a hospital in Varanasi, Uttar Pradesh where many cases of tapeworm were reported. After the successful extraction and lysis of the Tapeworm eggs, Berlin team obtained RNA molecules of the T. Solium species of the Tapeworm. IIT Delhi further assisted them by providing our own lab as -20 and -80 degrees centigrade refrigerators and other required lab apparatus were not available in the above-mentioned hospital. Sequencing of the RNA was also done and all the samples were shipped to them.<br>
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<h2 id="pfont1"><br>Following issues were discussed in the training for safety of lab:
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<ul>
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<li>Lab access and rules (including appropriate clothing, eating and drinking, etc.).
 +
<li>Responsible individuals (such as lab or departmental specialist or institutional biosafety officer).
 +
<li>Differences between biosafety levels.
 +
<li>Biosafety equipment (such as biosafety cabinets).
 +
<li>Good microbial technique (such as lab practices).
 +
<li>Disinfection and sterilization.
 +
<li>Emergency procedures.
 +
<li>Chemicals, fire and electrical safety.
 +
</ul><br>
 +
The issue of transport rules was not covered and therefore, we didn’t have enough idea about the same in the beginning. Later through the collaboration on GMM Legislation we got a good insight of the transport rules in various parts of the world. Here is the link:
 +
<br><a href="https://2017.igem.org/Team:IIT_Delhi/berlin"> https://2017.igem.org/Team:IIT_Delhi/berlin</a>
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 +
   
  
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This was not only constricted to iGEM collaboration as we further plan to adopt this technology for affordable diagnostic of tapeworm infection which is otherwise done using fecal inspection using a microscope. This would help in timely detection of infection and thus would save lives of many people. Following this, Berlin team provided us the protease stains without Degradation Tags required by us to generate oscillations in the form of a square. Here is the link to the iGEM Berlin Team.
 
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<a href="https://2017.igem.org/Team:Berlin_diagnostX/Collaborations"> https://2017.igem.org/Team:Berlin_diagnostX/Collaborations </a>
 
 
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           <button class="accordion back2" style="font-weight: bold;">Team: No work was conducted with Risk Group 3 or 4 organisms.</button>
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           <button class="accordion back2" style="font-weight: bold;">Risk Group and Safety Level laboratory 3 or 4</button>
 
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--IIT_Delhi--Glassgow.jpg" alt="" /></span><br> iGEM IIT Delhi assisted team Glasgow in 2016 for filling up survey forms designed for the people of India. This year iGEM Glasgow helped us by providing the biological parts ( pPhlF, RBS+PhlF + T, pSrpR, RBS + SrpR + T) which turned to be of immense use in forming the Oscillator.
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<h2 id="pfont1"><br> No work was conducted with Risk Group 3 or 4 organisms.<br><br>
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Chassis organism used : E.coli.<br>
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Strains of E.coli used : DH5-alpha, K-12, BL-21.<br>
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Description : Risk Group 1 organisms do not cause disease in healthy adult humans.<br>
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Lab Safety Level : Safety Level 1.<br>
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<br>
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Besides our chassis, we used Streptomyces griseus (Orf2), Bacillus megaterium, Pseudomonas putida(SrpR) , Pseudomonas fluorescens (PhlF) and Bacillus subtilis (LmrA)  because we are interested  in repressor proteins expressed in these micro-organism. We have found the sequence and had it synthesized by a provider or fish it out from the organisms using PCR.
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<br><br>
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Since The project is not associated with Risk Group 3 and 4 Organisms and therefore no work was conducted by the team in a Safety Level 3 or 4 laboratory.
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<button class="accordion back3" style="font-weight: bold;">No research was conducted by the team in a Safety Level 3 or 4 laboratory.</button>
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<button class="accordion back3" style="font-weight: bold;">Safe Shipment Guidelines
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/2/23/T--IIT_Delhi--iGEM_mohali.jpg" alt="" /></span>A PhD student, Mr. Mahendra Sahare, doing a humanities project under a Supreme Court lawyer, Dr. Naveen Thayyil, has been in collaboration with iGEM IIT Delhi for the past two years. Together, we have worked on the aspect of ‘Ethical Scientific Practices’. Also, on his recent visit to IISER Mohali, he came back with a collaborative offer for iGEM IIT Delhi to mentor IISER Mohali and help in the establishment of iGEM club there. On our return from the Giant Jamboree 2016, our team members gave a presentation scheduled there, and thereafter our
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<h2 id="pfont1">iGEM IIT Delhi has always followed safe shipment guidelines while submitting samples. Certificates has always been processed and verified before any shipment. Here is ‘Non Infectious and Non Hazardous Certificate’ attached  for shipment of one of the DNA samples.<br>
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team mentored the iGEM Mohali team.
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Here is the link for Mohali iGEM Team: <a href="https://2017.igem.org/Team:IISER-Mohali-INDIA"> https://2017.igem.org/Team:IISER-Mohali-INDIA </a>
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<a href = "https://static.igem.org/mediawiki/2017/1/1a/T--IIT_Delhi--Certificate_safety.pdf">https://static.igem.org/mediawiki/2017/1/1a/T--IIT_Delhi--Certificate_safety.pdf</a>
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<button class="accordion back4" style="font-weight: bold;">Team: MANCHESTER </button>
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/3/3e/T--IIT_Delhi--iGEM_Manchester.jpg" alt="" /></span>Have a look at the fruits of the 3 months-long interactions, discussions and meet-ups at <a href="https://2017.igem.org/Team:IIT_Delhi/GMM_legislation"> https://2017.igem.org/Team:IIT_Delhi/GMM_legislation </a>.
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<h2 id="pfont1">iGEM IIT Delhi has not  released or deployed our project outside of the laboratory at any time during the competition or at the Giant Jamboree.
  
 
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--IIT_Delhi--Berlin.jpg" alt="" /></span><br> The IIT Delhi iGEM team whole-heartedly collaborated with Berlin team. Berlin team was working on the affordable, practical and effective diagnostic of the widespread third world parasitic disease -Tapeworm Infection. Since India is one of the countries where millions of people are infected by this parasite, IIT Delhi directed Berlin team to a suitable site. It was a hospital in Varanasi, Uttar Pradesh where many cases of tapeworm were reported. After the successful extraction and lysis of the Tapeworm eggs, Berlin team obtained RNA molecules of the T. Solium species of the Tapeworm. IIT Delhi further assisted them by providing our own lab as -20 and -80 degrees centigrade refrigerators and other required lab apparatus were not available in the above-mentioned hospital. Sequencing of the RNA was also done and all the samples were shipped to them.<br>
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<h2 id="pfont1"><br> iGEM IIT Delhi has always been following all biosafety rules of our institution and concerned biosafety laws of our country.<br><br>
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 +
EHLSU (Environmental Health and Lab Safety Unit) is responsible for holding up all the safety guidelines in all the labs (including ours) at the institution. The guidelines can be found on the link -<br>
 +
    <a href = "http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf">http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf</a>
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<br><br>
 +
The safety guidelines for all the labs in our region (North India) are declared and monitored by EHLSU, whose rules can be found on the following link - <br>
 +
<a href = "http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf">http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf<br></a>
 +
iGEM IIT Delhi did not conduct any experiment with human subjects (including non-invasive experiments, such as surveys), for the surveys conducted we have followed the rules of our institution.
  
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This was not only constricted to iGEM collaboration as we further plan to adopt this technology for affordable diagnostic of tapeworm infection which is otherwise done using fecal inspection using a microscope. This would help in timely detection of infection and thus would save lives of many people. Following this, Berlin team provided us the protease stains without Degradation Tags required by us to generate oscillations in the form of a square. Here is the link to the iGEM Berlin Team.
 
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<a href="https://2017.igem.org/Team:Berlin_diagnostX/Collaborations"> https://2017.igem.org/Team:Berlin_diagnostX/Collaborations </a>
 
 
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--IIT_Delhi--Berlin.jpg" alt="" /></span><br> The IIT Delhi iGEM team whole-heartedly collaborated with Berlin team. Berlin team was working on the affordable, practical and effective diagnostic of the widespread third world parasitic disease -Tapeworm Infection. Since India is one of the countries where millions of people are infected by this parasite, IIT Delhi directed Berlin team to a suitable site. It was a hospital in Varanasi, Uttar Pradesh where many cases of tapeworm were reported. After the successful extraction and lysis of the Tapeworm eggs, Berlin team obtained RNA molecules of the T. Solium species of the Tapeworm. IIT Delhi further assisted them by providing our own lab as -20 and -80 degrees centigrade refrigerators and other required lab apparatus were not available in the above-mentioned hospital. Sequencing of the RNA was also done and all the samples were shipped to them.<br>
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<h2 id="pfont1"><br><ul><li> Instructions are strictly followed.<br><li> Personal protection equipment is must and hair should be tied.<br><li>
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+
Working areas and common work areas are kept tidy and regularly wiped with cleansing agents like ethanol.<br><li>
−
This was not only constricted to iGEM collaboration as we further plan to adopt this technology for affordable diagnostic of tapeworm infection which is otherwise done using fecal inspection using a microscope. This would help in timely detection of infection and thus would save lives of many people. Following this, Berlin team provided us the protease stains without Degradation Tags required by us to generate oscillations in the form of a square. Here is the link to the iGEM Berlin Team.
+
Pipette tips, media, eppendorf tubes are autoclaved and Glassware; tools are cleaned with ethanol before usage.<br><li>
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<a href="https://2017.igem.org/Team:Berlin_diagnostX/Collaborations"> https://2017.igem.org/Team:Berlin_diagnostX/Collaborations </a>
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Handwash is a must after entering and before leaving the lab.<br><li>
 +
Gloves are used while handling carcinogenic agents like EtBr and high temperature objects. <br><li>
 +
There is a separate work bench for experiments in which toxic chemicals (e.g. Electrophoresis, Etbr).   <br><li> 
 +
Eating or drinking is prohibited in the lab.<br><li>
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Spills are immediately cleaned.<br><li>
 +
Lab is equipped with safety equipment and everyone is aware of its location (E.g. Fire extinguishers and first aid supplies).<br><li>
 +
Appropriate protection is used while working with UV light(eye protection, gloves, lab coat).<br><li>
 +
Lab waste is properly disposed (E.g. Biological cultures are disposed after addition of ethanol to kill micro-organisms; Solids are kept out of the sink; Agarose gel, gloves, the hazardous wastes are disposed separately in the bio-hazard coloured bin). <br>          
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--IIT_Delhi--Berlin.jpg" alt="" /></span><br> The IIT Delhi iGEM team whole-heartedly collaborated with Berlin team. Berlin team was working on the affordable, practical and effective diagnostic of the widespread third world parasitic disease -Tapeworm Infection. Since India is one of the countries where millions of people are infected by this parasite, IIT Delhi directed Berlin team to a suitable site. It was a hospital in Varanasi, Uttar Pradesh where many cases of tapeworm were reported. After the successful extraction and lysis of the Tapeworm eggs, Berlin team obtained RNA molecules of the T. Solium species of the Tapeworm. IIT Delhi further assisted them by providing our own lab as -20 and -80 degrees centigrade refrigerators and other required lab apparatus were not available in the above-mentioned hospital. Sequencing of the RNA was also done and all the samples were shipped to them.<br>
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This was not only constricted to iGEM collaboration as we further plan to adopt this technology for affordable diagnostic of tapeworm infection which is otherwise done using fecal inspection using a microscope. This would help in timely detection of infection and thus would save lives of many people. Following this, Berlin team provided us the protease stains without Degradation Tags required by us to generate oscillations in the form of a square. Here is the link to the iGEM Berlin Team.
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<a href="https://2017.igem.org/Team:Berlin_diagnostX/Collaborations"> https://2017.igem.org/Team:Berlin_diagnostX/Collaborations </a>
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--IIT_Delhi--Berlin.jpg" alt="" /></span><br> The IIT Delhi iGEM team whole-heartedly collaborated with Berlin team. Berlin team was working on the affordable, practical and effective diagnostic of the widespread third world parasitic disease -Tapeworm Infection. Since India is one of the countries where millions of people are infected by this parasite, IIT Delhi directed Berlin team to a suitable site. It was a hospital in Varanasi, Uttar Pradesh where many cases of tapeworm were reported. After the successful extraction and lysis of the Tapeworm eggs, Berlin team obtained RNA molecules of the T. Solium species of the Tapeworm. IIT Delhi further assisted them by providing our own lab as -20 and -80 degrees centigrade refrigerators and other required lab apparatus were not available in the above-mentioned hospital. Sequencing of the RNA was also done and all the samples were shipped to them.<br>
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This was not only constricted to iGEM collaboration as we further plan to adopt this technology for affordable diagnostic of tapeworm infection which is otherwise done using fecal inspection using a microscope. This would help in timely detection of infection and thus would save lives of many people. Following this, Berlin team provided us the protease stains without Degradation Tags required by us to generate oscillations in the form of a square. Here is the link to the iGEM Berlin Team.
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<h2 id="pfont1"><span class="image left"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--IIT_Delhi--Berlin.jpg" alt="" /></span><br> The IIT Delhi iGEM team whole-heartedly collaborated with Berlin team. Berlin team was working on the affordable, practical and effective diagnostic of the widespread third world parasitic disease -Tapeworm Infection. Since India is one of the countries where millions of people are infected by this parasite, IIT Delhi directed Berlin team to a suitable site. It was a hospital in Varanasi, Uttar Pradesh where many cases of tapeworm were reported. After the successful extraction and lysis of the Tapeworm eggs, Berlin team obtained RNA molecules of the T. Solium species of the Tapeworm. IIT Delhi further assisted them by providing our own lab as -20 and -80 degrees centigrade refrigerators and other required lab apparatus were not available in the above-mentioned hospital. Sequencing of the RNA was also done and all the samples were shipped to them.<br>
 
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This was not only constricted to iGEM collaboration as we further plan to adopt this technology for affordable diagnostic of tapeworm infection which is otherwise done using fecal inspection using a microscope. This would help in timely detection of infection and thus would save lives of many people. Following this, Berlin team provided us the protease stains without Degradation Tags required by us to generate oscillations in the form of a square. Here is the link to the iGEM Berlin Team.
 
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<a href="https://2017.igem.org/Team:Berlin_diagnostX/Collaborations"> https://2017.igem.org/Team:Berlin_diagnostX/Collaborations </a>
 
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Latest revision as of 23:26, 1 November 2017

iGEM IIT Delhi

Overview Squarewave Generator Interlab
Circuit design and construction Microfluidics and Fluorescence Photobleaching Promoter strength Oscillations
Basic Parts Composite Parts Improved Parts Part Collection
Overview Writing a Model Deterministic Model Stochastic Model Bifurcation and Squareness Resource sharing
Human Practices Integrated Practices Public Engagement Collaborations Safety
Overview GMM Legislation iGEM Berlin Mentoring IISER Mohali iGEM Glasgow
Lab Records Protocols Recipes Medal Criteria
The Team Attributions

Safety

                                                                                                                                                                                                                 

iGEM IIT Delhi follows a high standard of safe and responsible biological engineering. We completely understand that we are responsible for living up to the trust placed in us to design, build, and share biological devices safely.


Following issues were discussed in the training for safety of lab:
  • Lab access and rules (including appropriate clothing, eating and drinking, etc.).
  • Responsible individuals (such as lab or departmental specialist or institutional biosafety officer).
  • Differences between biosafety levels.
  • Biosafety equipment (such as biosafety cabinets).
  • Good microbial technique (such as lab practices).
  • Disinfection and sterilization.
  • Emergency procedures.
  • Chemicals, fire and electrical safety.

The issue of transport rules was not covered and therefore, we didn’t have enough idea about the same in the beginning. Later through the collaboration on GMM Legislation we got a good insight of the transport rules in various parts of the world. Here is the link:
https://2017.igem.org/Team:IIT_Delhi/berlin



No work was conducted with Risk Group 3 or 4 organisms.

Chassis organism used : E.coli.
Strains of E.coli used : DH5-alpha, K-12, BL-21.
Description : Risk Group 1 organisms do not cause disease in healthy adult humans.
Lab Safety Level : Safety Level 1.

Besides our chassis, we used Streptomyces griseus (Orf2), Bacillus megaterium, Pseudomonas putida(SrpR) , Pseudomonas fluorescens (PhlF) and Bacillus subtilis (LmrA) because we are interested in repressor proteins expressed in these micro-organism. We have found the sequence and had it synthesized by a provider or fish it out from the organisms using PCR.

Since The project is not associated with Risk Group 3 and 4 Organisms and therefore no work was conducted by the team in a Safety Level 3 or 4 laboratory.


iGEM IIT Delhi has always followed safe shipment guidelines while submitting samples. Certificates has always been processed and verified before any shipment. Here is ‘Non Infectious and Non Hazardous Certificate’ attached for shipment of one of the DNA samples.
https://static.igem.org/mediawiki/2017/1/1a/T--IIT_Delhi--Certificate_safety.pdf


iGEM IIT Delhi has not released or deployed our project outside of the laboratory at any time during the competition or at the Giant Jamboree.



iGEM IIT Delhi has always been following all biosafety rules of our institution and concerned biosafety laws of our country.

EHLSU (Environmental Health and Lab Safety Unit) is responsible for holding up all the safety guidelines in all the labs (including ours) at the institution. The guidelines can be found on the link -
http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf

The safety guidelines for all the labs in our region (North India) are declared and monitored by EHLSU, whose rules can be found on the following link -
http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf
 iGEM IIT Delhi did not conduct any experiment with human subjects (including non-invasive experiments, such as surveys), for the surveys conducted we have followed the rules of our institution.



  • Instructions are strictly followed.
  • Personal protection equipment is must and hair should be tied.
  • Working areas and common work areas are kept tidy and regularly wiped with cleansing agents like ethanol.
  • Pipette tips, media, eppendorf tubes are autoclaved and Glassware; tools are cleaned with ethanol before usage.
  • Handwash is a must after entering and before leaving the lab.
  • Gloves are used while handling carcinogenic agents like EtBr and high temperature objects.
  • There is a separate work bench for experiments in which toxic chemicals (e.g. Electrophoresis, Etbr).
  • Eating or drinking is prohibited in the lab.
  • Spills are immediately cleaned.
  • Lab is equipped with safety equipment and everyone is aware of its location (E.g. Fire extinguishers and first aid supplies).
  • Appropriate protection is used while working with UV light(eye protection, gloves, lab coat).
  • Lab waste is properly disposed (E.g. Biological cultures are disposed after addition of ethanol to kill micro-organisms; Solids are kept out of the sink; Agarose gel, gloves, the hazardous wastes are disposed separately in the bio-hazard coloured bin).






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Contact Us Address

E-mail: iitd.igem@gmail.com
Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi