Difference between revisions of "Team:Dalhousie/Experiments"
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| − | < | + | <h1 id="FecalSamples" style="color: white;"> Protocols </br></br></h1> |
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<h4><a href="https://static.igem.org/mediawiki/2017/f/fa/Dalfluoro.pdf" style="color: rgba(193,211,93,0.8);"> Fluorophore Assessment of Glycosidase Activity </a></h4> | <h4><a href="https://static.igem.org/mediawiki/2017/f/fa/Dalfluoro.pdf" style="color: rgba(193,211,93,0.8);"> Fluorophore Assessment of Glycosidase Activity </a></h4> | ||
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| − | <h4><a href="https://static.igem.org/mediawiki/2017/a/af/Dalcosmid.pdf" style="color: rgba(193,211,93,0.8);">Cosmid Library Generation | + | <h4><a href="https://static.igem.org/mediawiki/2017/a/af/Dalcosmid.pdf" style="color: rgba(193,211,93,0.8);">Cosmid Library Generation </br></br> |
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| − | <p>To visual total protein in a given sample</br></br></br></br></br><p> | + | <p>To visual total protein in a given sample.</br></br></br></br></br><p> |
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| − | <p>To identify specific amino acid sequences of a protein, or tag, using fluorescently-tagged antibodies.<p> | + | <p>To identify specific amino acid sequences of a protein, or tag, using fluorescently-tagged antibodies.</br></br></br><p> |
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| − | <p>Purpose of this protocol is to grow up single colonies of bacteria for use.<p> | + | <p>Purpose of this protocol is to grow up single colonies of bacteria for use.</br><p> |
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| − | <p>Purpose of this overview is to isolate and clone genes of interest into an appropriate plasmid and then introduce the recombinant DNA molecule into E. coli. | + | <p>Purpose of this overview is to isolate and clone genes of interest into an appropriate plasmid and then introduce the recombinant DNA molecule into <i>E. coli</i>. </br></br> |
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| − | <p>Concentration protocol to concentrate DNA isolating using the PowerFecal Extraction kit.<p> | + | <p>Concentration protocol to concentrate DNA isolating using the PowerFecal Extraction kit.</br></br></br><p> |
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| − | <h4><a href="https://static.igem.org/mediawiki/2017/9/9d/Dalgelmaking.pdf" style="color: rgba(193,211,93,0.8);"> 0.8% Agarose Gel | + | <h4><a href="https://static.igem.org/mediawiki/2017/9/9d/Dalgelmaking.pdf" style="color: rgba(193,211,93,0.8);"> 0.8% Agarose Gel </br></br> |
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| − | <p>Purpose of this protocol is to create an agarose gel for visualizing and extracting DNA.<p> | + | <p>Purpose of this protocol is to create an agarose gel for visualizing and extracting DNA.</br></br></br><p> |
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| − | <p>The purpose of this protocol is to create solid growth media containing cellobiose to measure beta-glucosidase activity.<p> | + | <p>The purpose of this protocol is to create solid growth media containing cellobiose to measure beta-glucosidase activity.</br></br></br><p> |
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| − | <p>Purpose of this protocol is to introduce plasmid DNA into E. coli cells. | + | <p>Purpose of this protocol is to introduce plasmid DNA into <i>E. coli</i> cells.</br></br></br> |
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| − | <h4><a href="https://static.igem.org/mediawiki/2017/ | + | <h4><a href="https://static.igem.org/mediawiki/2017/0/09/Dalsalt.pdf" style="color: rgba(193,211,93,0.8);"> M9 Salts Generation |
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| − | <p>Purpose of this protocol is to generate | + | <p>Purpose of this protocol is to generate a salt solution for <i>E. coli</i> growth. </br></br></br><p> |
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Latest revision as of 23:33, 1 November 2017
Protocols
Purpose of this protocol is to assess the enzymatic activity of glycosidases using fluorophores conjugated to substrates of interest (such as cellobiose or xylobiose). Once the enzyme of interest cleaves substrate, the fluorophore is released enabling quantification of enzymatic activity.
Purpose of this protocol is to generate a library of cosmid clones that are representative of the microbiome of the porcupine for screening for novel enzymes.
To identify specific amino acid sequences of a protein, or tag, using fluorescently-tagged antibodies.
Purpose of this protocol is to grow up single colonies of bacteria for use.
Purpose of this overview is to isolate and clone genes of interest into an appropriate plasmid and then introduce the recombinant DNA molecule into E. coli.
The purpose of this protocol is to assay carboxymethyl cellulose degradation via a pH change detectable by the dye Congo Red as differentially coloured halos around colonies of bacteria.
Concentration protocol to concentrate DNA isolating using the PowerFecal Extraction kit.
Purpose of this protocol is to create an agarose gel for visualizing and extracting DNA.
The purpose of this protocol is to create solid growth media containing cellobiose to measure beta-glucosidase activity.
Purpose of this protocol is to generate solid growth media containing LB and an antibiotic of choice for selective growth of successful clones following transformation.
The purpose of this protocol is to create solid growth media containing both carboxymethylcellulose and glucose for measuring endoglucanase activity.
