Difference between revisions of "Team:DTU-Denmark/Model"

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           <p>You can find the plots of logarithmic fluorescence signal and time of all the substrates <a href="https://static.igem.org/mediawiki/2017/a/a9/T--DTU-Denmark--modelling_all_raw.pdf">here</a></p><br />
 
           <p>You can find the plots of logarithmic fluorescence signal and time of all the substrates <a href="https://static.igem.org/mediawiki/2017/a/a9/T--DTU-Denmark--modelling_all_raw.pdf">here</a></p><br />
  
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          <p>The relative signal of each substrate between the three snakes was also analyzed. You can find that part <a href="https://static.igem.org/mediawiki/2017/d/d9/T--DTU-Denmark--modelling_all_logfc.pdf">here</a></p><br />
  
 
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          <p>Lastly, we looked at the fluorescence output in terms of enzymatic activity. We fit a non-linear model in the reactions and calculated their rates. You can find the models for all the substrates <a href="https://static.igem.org/mediawiki/2017/2/2d/T--DTU-Denmark--modelling_all_signal_by_log.pdf">here</a></p><br />
  
  

Revision as of 00:09, 2 November 2017

Substrate Set

Our substrate screening experiment produced a large number of interesting substrates for our detection assay. We looked at the raw fluorescence measurements, but we also applied statistical analysis to identify the peptides that had significant differences between the three venoms. In addition, we modelled the enzymatic activity of the venoms based on our measurements.


Venn Diagram
Figure 1: Significant substrates found in our experiment, by looking at the signal difference between the three snakes after the 60 minutes timepoint. BA represents Bitis arietans, BG represents Bitis gabonica and NN Naja nigricollis. Significance here is defined by log10(p) < -9.

You can find the plots of logarithmic fluorescence signal and time of all the substrates here


The relative signal of each substrate between the three snakes was also analyzed. You can find that part here


Lastly, we looked at the fluorescence output in terms of enzymatic activity. We fit a non-linear model in the reactions and calculated their rates. You can find the models for all the substrates here


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