Difference between revisions of "Team:NUDT CHINA/Part Collection"
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<div class="pure-menu"><img src="https://static.igem.org/mediawiki/2017/4/4f/T-NUDT_CHINA-icon01.png" width=240px height=190px alt=""> | <div class="pure-menu"><img src="https://static.igem.org/mediawiki/2017/4/4f/T-NUDT_CHINA-icon01.png" width=240px height=190px alt=""> | ||
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<ul class="pure-menu-children" id="team_show"> | <ul class="pure-menu-children" id="team_show"> | ||
<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Team" class="pure-menu-link">Team</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Team" class="pure-menu-link">Team</a></li> | ||
| − | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Collaborations" class="pure-menu-link">Collaborations</a></li> | + | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Collaborations" class="pure-menu-link">Collaborations</a></li> |
</ul> | </ul> | ||
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<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Experiments" class="pure-menu-link">Experiment</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Experiments" class="pure-menu-link">Experiment</a></li> | ||
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<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Results" class="pure-menu-link">Results</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Results" class="pure-menu-link">Results</a></li> | ||
<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Demonstrate" class="pure-menu-link">Demonstrate</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Demonstrate" class="pure-menu-link">Demonstrate</a></li> | ||
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<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Basic_Part" class="pure-menu-link">Basic Parts</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Basic_Part" class="pure-menu-link">Basic Parts</a></li> | ||
<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Composite_Part" class="pure-menu-link">Composite Parts</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Composite_Part" class="pure-menu-link">Composite Parts</a></li> | ||
| − | <li class="pure-menu-item"><a class="pure-menu-heading" | + | <li class="pure-menu-item"><a class="pure-menu-heading" href="https://2017.igem.org/Team:NUDT_CHINA/Part_Collection" class="pure-menu-link">Part Collection</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| + | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Attributions" class="pure-menu-link">Attributions</a></li> | ||
| + | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/InterLab" class="pure-menu-link">InterLab</a></li> | ||
| + | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Model" class="pure-menu-link">Model</a></li> | ||
<li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Safety" class="pure-menu-link">SAFETY</a></li> | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/Safety" class="pure-menu-link">SAFETY</a></li> | ||
<li class="pure-menu-item pure-menu-has-children"> | <li class="pure-menu-item pure-menu-has-children"> | ||
<a class="pure-menu-link"onclick="Show('HP_show')">HP</a> | <a class="pure-menu-link"onclick="Show('HP_show')">HP</a> | ||
<ul class="pure-menu-children" id="HP_show"> | <ul class="pure-menu-children" id="HP_show"> | ||
| − | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/HP/Silver" class="pure-menu-link"> | + | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/HP/Silver" class="pure-menu-link">Silver HP</a></li> |
| − | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/HP/Gold_Integrated" class="pure-menu-link">Integrated | + | <li class="pure-menu-item"><a href="https://2017.igem.org/Team:NUDT_CHINA/HP/Gold_Integrated" class="pure-menu-link">Integrated & Gold</a></li> |
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<div class="content" style="width: 74%"> | <div class="content" style="width: 74%"> | ||
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| + | <style> | ||
| + | table td{height: 30px;font-size: 30px;} | ||
| + | table th{height: 45px;font-size: 15px;} | ||
| + | </style> | ||
| + | <p class="content-subhead">Part list</p> | ||
| + | <p >This part collection is a collection of miRNA target sequences, contained 26 new parts designed for miRNA locker assembly. In this collection, we collected reverse complementary sequence of miRNAs. Building on that, we can obtain modularized DNA parts by annealing, and then construct a set of chemically synthetic oligo DNA library. Our miRNA locker can be easily assembled using modularized DNA parts from this chemically synthetic oligo DNA library. Most of the parts in this kit were functional validated and submitted to the registry, expect for BBa_K2440016 and BBa_K2440034, as they are not compatible with RFC10.</p> | ||
| + | <center> | ||
| + | <table class=" pgrouptable tablesorter" style="width: 80%;" cellpadding="0" cellspacing="0"> | ||
| + | <thead> | ||
| + | <tr><th style="width:8px" class="header"></th><th style="width:8px" class="header"></th><th style="width:8px" class="header headerSortUp"></th><th style="width: 87px;" class="header">Name</th><th style="width: 80px" colspan="" class="header">Type</th><th style="width: auto;" colspan="" class="header">Description</th><th style="width: 150px" colspan="" class="header">Designer</th><th style="width: 50px" colspan="" class="header">Length</th> | ||
| + | </tr></thead><tbody> | ||
| + | |||
| + | <tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440011">BBa_K2440011</a></td><td>DNA</td><td>let-7a target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440012">BBa_K2440012</a></td><td>DNA</td><td>miR-4470 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440013">BBa_K2440013</a></td><td>DNA</td><td>miR-16 target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440014">BBa_K2440014</a></td><td>DNA</td><td>miR-195 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440015">BBa_K2440015</a></td><td>DNA</td><td>miR-654 target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440016">BBa_K2440016</a></td><td>DNA</td><td>miR-511 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440017">BBa_K2440017</a></td><td>DNA</td><td>miR-875 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440018">BBa_K2440018</a></td><td>DNA</td><td>miR-384 target sequence</td><td width="100px">Qijie Xu</td><td align="right">20</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440019">BBa_K2440019</a></td><td>DNA</td><td>miR-2467 target sequence</td><td width="100px">Qijie Xu</td><td align="right">23</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440020">BBa_K2440020</a></td><td>DNA</td><td>miR-184 target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440021">BBa_K2440021</a></td><td>DNA</td><td>miR2118l target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440022">BBa_K2440022</a></td><td>DNA</td><td>miR418 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440023">BBa_K2440023</a></td><td>DNA</td><td>miR1441 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440024">BBa_K2440024</a></td><td>DNA</td><td>miR817 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440025">BBa_K2440025</a></td><td>DNA</td><td>miR165a target sequence</td><td width="100px">Qijie Xu</td><td align="right">20</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440026">BBa_K2440026</a></td><td>DNA</td><td>miR160b target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440027">BBa_K2440027</a></td><td>DNA</td><td>miR397b target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440028">BBa_K2440028</a></td><td>DNA</td><td>miR4249 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440029">BBa_K2440029</a></td><td>DNA</td><td>miR-458 target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440030">BBa_K2440030</a></td><td>DNA</td><td>miR-152 target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440031">BBa_K2440031</a></td><td>DNA</td><td>miR-155 target sequence</td><td width="100px">Qijie Xu</td><td align="right">22</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440032">BBa_K2440032</a></td><td>DNA</td><td>miR-489 target sequence</td><td width="100px">Qijie Xu</td><td align="right">23</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440033">BBa_K2440033</a></td><td>DNA</td><td>miR3476 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440034">BBa_K2440034</a></td><td>DNA</td><td>miR172 target sequence</td><td width="100px">Qijie Xu</td><td align="right">21</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440035">BBa_K2440035</a></td><td>DNA</td><td>miR7491 target sequence</td><td width="100px">Qijie Xu</td><td align="right">24</td> | ||
| + | </tr><tr> | ||
| + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2440036">BBa_K2440036</a></td><td>DNA</td><td>miR7514 target sequence</td><td width="100px">Qijie Xu</td><td align="right">24</td> | ||
| + | </tr></tbody> | ||
| + | </table></center> | ||
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</div> | </div> | ||
</div></div> | </div></div> | ||
Latest revision as of 00:10, 2 November 2017
Part Collection
Part list
This part collection is a collection of miRNA target sequences, contained 26 new parts designed for miRNA locker assembly. In this collection, we collected reverse complementary sequence of miRNAs. Building on that, we can obtain modularized DNA parts by annealing, and then construct a set of chemically synthetic oligo DNA library. Our miRNA locker can be easily assembled using modularized DNA parts from this chemically synthetic oligo DNA library. Most of the parts in this kit were functional validated and submitted to the registry, expect for BBa_K2440016 and BBa_K2440034, as they are not compatible with RFC10.
| Name | Type | Description | Designer | Length | |||
|---|---|---|---|---|---|---|---|
| BBa_K2440011 | DNA | let-7a target sequence | Qijie Xu | 22 | |||
| BBa_K2440012 | DNA | miR-4470 target sequence | Qijie Xu | 21 | |||
| BBa_K2440013 | DNA | miR-16 target sequence | Qijie Xu | 22 | |||
| BBa_K2440014 | DNA | miR-195 target sequence | Qijie Xu | 21 | |||
| BBa_K2440015 | DNA | miR-654 target sequence | Qijie Xu | 22 | |||
| BBa_K2440016 | DNA | miR-511 target sequence | Qijie Xu | 21 | |||
| BBa_K2440017 | DNA | miR-875 target sequence | Qijie Xu | 21 | |||
| BBa_K2440018 | DNA | miR-384 target sequence | Qijie Xu | 20 | |||
| BBa_K2440019 | DNA | miR-2467 target sequence | Qijie Xu | 23 | |||
| BBa_K2440020 | DNA | miR-184 target sequence | Qijie Xu | 22 | |||
| BBa_K2440021 | DNA | miR2118l target sequence | Qijie Xu | 22 | |||
| BBa_K2440022 | DNA | miR418 target sequence | Qijie Xu | 21 | |||
| BBa_K2440023 | DNA | miR1441 target sequence | Qijie Xu | 21 | |||
| BBa_K2440024 | DNA | miR817 target sequence | Qijie Xu | 21 | |||
| BBa_K2440025 | DNA | miR165a target sequence | Qijie Xu | 20 | |||
| BBa_K2440026 | DNA | miR160b target sequence | Qijie Xu | 22 | |||
| BBa_K2440027 | DNA | miR397b target sequence | Qijie Xu | 21 | |||
| BBa_K2440028 | DNA | miR4249 target sequence | Qijie Xu | 21 | |||
| BBa_K2440029 | DNA | miR-458 target sequence | Qijie Xu | 22 | |||
| BBa_K2440030 | DNA | miR-152 target sequence | Qijie Xu | 22 | |||
| BBa_K2440031 | DNA | miR-155 target sequence | Qijie Xu | 22 | |||
| BBa_K2440032 | DNA | miR-489 target sequence | Qijie Xu | 23 | |||
| BBa_K2440033 | DNA | miR3476 target sequence | Qijie Xu | 21 | |||
| BBa_K2440034 | DNA | miR172 target sequence | Qijie Xu | 21 | |||
| BBa_K2440035 | DNA | miR7491 target sequence | Qijie Xu | 24 | |||
| BBa_K2440036 | DNA | miR7514 target sequence | Qijie Xu | 24 |
