Difference between revisions of "Team:Cadets2Vets"

 
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{{Cadets2Vets}}
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{{Team:Cadets2Vets/SideMenu}}
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Cadets 2 Vets</title>
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<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
 +
</a>
 +
 +
</li>
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 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="safety.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="team.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-8308751370782bc"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="basic-parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-78fbd0423085ce2"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-24ea54f35798e9a"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Composite Parts</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-8d68d4dbac38a10"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="interlab.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-c92600692c09125"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Interlab</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="our-partners.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-be35f8069be1925"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Our Partners</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-september.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="2d522622"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Environmental Project</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="hardware.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="results.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
 +
</a>
 +
 +
</li>
 +
 
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
 +
</a>
 
 
 
</li>
 
</li>
Line 169: Line 403:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-6d81c86e-aks9i9z5/vbid-f1559d17-lok1anrm" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="7ef9726b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team Members</span>
+
<span id="element-563894f97a8ebaf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
 
</a>
 
</a>
 
 
Line 180: Line 414:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-6d81c86e-aks9i9z5/vbid-1590a5fc-vat3ttnl" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="integrated-and-gold.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="884bbf93"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Projects</span>
+
<span id="element-874635e92416b44"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 
</a>
 
</a>
 
 
Line 210: Line 444:
 
data-MENU_SCROLL="false"
 
data-MENU_SCROLL="false"
 
data-ALWAYS_MINIFIED="false"
 
data-ALWAYS_MINIFIED="false"
−
data-MENU_POSITION="top"
+
data-MENU_POSITION="none"
 
data-MENU_ALIGN="left"
 
data-MENU_ALIGN="left"
 
data-BACKGROUND_COLOR="rgb(124, 100, 41)"
 
data-BACKGROUND_COLOR="rgb(124, 100, 41)"
Line 224: Line 458:
 
 
 
<!-- FORM START -->
 
<!-- FORM START -->
−
<div  id="vbid-6d81c86e-kmehihke"  class="master item-box  page-box style-6d81c86e-vkacomud   button-effects btn_hover3      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6d81c86e-vkacomud" data-preview-styleid='style-6d81c86e-vkacomud' data-preset-type-id="FORM">
+
<div  id="vbid-59a5b-tjgooy1n"  class="master item-box  page-box style-59a5b-ti3xte4k   button-effects btn_hover3      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-ti3xte4k" data-preview-styleid='style-59a5b-ti3xte4k' data-preset-type-id="FORM">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div class="page-wrapper item-wrapper form-wrapper ">
 
<div class="page-wrapper item-wrapper form-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6d81c86e-kmehihke" data-preview-style="style-6d81c86e-vkacomud" data-style="style-e4cfb-6huavktiba" data-orig-thumb-height="2416" data-orig-thumb-width="3500" data-vbid="vbid-6d81c86e-kmehihke" data-bgimg="https://lh3.googleusercontent.com/OAbUSoGAxLob9fusdd8GqWFGv8xOhxRhN3y6KLxpoOONepmYgG9ci5kUkDm8vdWLy4-MU9yUZyUUZ-vNuFk">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-tjgooy1n" data-preview-style="style-59a5b-ti3xte4k" data-style="style-e4cfb-6huavktiba" data-orig-thumb-height="2416" data-orig-thumb-width="3500" data-vbid="vbid-59a5b-tjgooy1n" data-bgimg="https://static.igem.org/mediawiki/2017/7/7c/Army_in_Parade_Dress.jpeg">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
Line 238: Line 472:
 
<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
<div class="vertical-aligner">
−
<div id="vbid-6d81c86e-5usv34hg-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-59a5b-nnn4mbrq-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div  id="vbid-6d81c86e-5usv34hg" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/OAbUSoGAxLob9fusdd8GqWFGv8xOhxRhN3y6KLxpoOONepmYgG9ci5kUkDm8vdWLy4-MU9yUZyUUZ-vNuFk=s300);"  data-orig-width="3500" data-orig-height="2416" >
+
<div  id="vbid-59a5b-nnn4mbrq" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/7/7c/Army_in_Parade_Dress.jpeg);"  data-orig-width="3500" data-orig-height="2416" >
 
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Line 271: Line 505:
 
 
 
 
 +
 +
 +
<div class="preview-icon-holder Icon removable-parent order-handle" >
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<img id="element-2e144876c8f0e64" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://static.igem.org/mediawiki/2017/thumb/d/d2/Cadets2Vets_Logo.jpg/800px-Cadets2Vets_Logo.jpg.png" />
 +
 +
</div>
 +
 
 
 
 
Line 288: Line 530:
 
 
 
 
−
+
−
<br class="upper-line-break" />
+
−
<div class="preview-subtitle-holder removable-parent order-handle">
+
 +
 +
 +
 +
 +
</div>
 +
</div>
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</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
<!-- FORM END -->
 +
 +
 
 
−
<h3 id="vbid-6d81c86e-4cch6exs" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Welcome to our Homepage</h3>
+
 +
<!-- MAPS START -->
 +
<div  id="vbid-59a5b-qihkumq9" class="master item-box  gallery-box style-59a5b-jqfr7efo    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="MAPS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
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<div class="gallery-wrapper item-wrapper maps-wrapper ">
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<div  class="sub container style-59a5b-jqfr7efo content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-jqfr7efo" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-qihkumq9" data-preset-type-id="MAPS" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
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data-ARRANGER_ITEM_MAX_WIDTH="400"
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data-ARRANGER_ITEM_RATIO="1"
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data-ARRANGER_ITEM_SPACING="20"
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data-ARRANGER_ITEMS_PER_PAGE="all"
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data-ARRANGER_ORDER_TYPE="regular"
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data-AUTO_PLAY="AUTOPLAY"
 +
data-AUTO_PLAY_DURATION="4"
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data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
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<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
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<div class="page-wrapper item-wrapper ">
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<div class="Picture item-preview">
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 +
</div>
 +
<div class="helper-div middle-center" >
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<div class="pic-side">
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<div class="vertical-aligner">
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 +
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 +
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<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
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 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
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 +
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 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 
</div>
 
</div>
−
<br class="lower-line-break" />
+
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
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<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
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 +
<!--  BY SPECIFIC ORDER -->
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 +
 +
 +
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<div class="preview-icon-holder Icon removable-parent order-handle" >
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<img id="vbid-59a5b-qgicu05p" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://static.igem.org/mediawiki/2017/3/30/US_Army_Logo.jpg" />
 +
 +
</div>
  
 
 
Line 306: Line 650:
 
<div class="preview-title-holder removable-parent order-handle">
 
<div class="preview-title-holder removable-parent order-handle">
 
 
−
<h1 id="vbid-6d81c86e-bx64r9hz" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Cadets2Vets<br></h1>
+
<h2 id="vbid-59a5b-j6hw2jzr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
 
 
 
</div>
 
</div>
Line 318: Line 662:
 
 
 
<br class="upper-line-break" />
 
<br class="upper-line-break" />
−
<div class="preview-body-holder removable-parent order-handle">
+
<div class="preview-subtitle-holder removable-parent order-handle">
−
+
−
<div id="vbid-6d81c86e-xcouhn7z" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu custom  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+
−
<p style="text-align: center;">- 2017 iGEM Team -</p>
+
−
</div>
+
 
 
 +
<h3 id="vbid-59a5b-potfnoot" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
 +
 
</div>
 
</div>
 
<br class="lower-line-break" />
 
<br class="lower-line-break" />
Line 331: Line 673:
 
 
 
 
 +
 +
<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
 +
 +
<span id="vbid-59a5b-hgd5ta77"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
 +
 +
</div>
 
 
 
 
Line 349: Line 697:
 
</div>
 
</div>
 
</div>
 
</div>
−
<!-- FORM END -->
+
 
 
 
 
 
 
 
 
−
<!-- MAPS START -->
+
−
<div  id="vbid-6e43f075-usun659t"  class="master item-box  gallery-box style-6e43f075-xcdlyuvv   button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-6e43f075-xcdlyuvv" data-preview-styleid='style-6e43f075-xcdlyuvv' data-preset-type-id="MAPS">
+
<div  id="vbid-59a5b-ma3fusho"  class="sub item-box  page-box style-59a5b-jqfr7efo          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-ma3fusho" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2952" data-orig-thumb-width="5248" data-vbid="vbid-59a5b-ma3fusho" data-bgimg="https://static.igem.org/mediawiki/2017/8/8c/Group_Photo.jpgC8tBbDNwQs5kZXRkYWwbVMMpLNUA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">As outlined by measure 7061A from the </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">Environmental Protection Agency</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample. </span></span></span></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions,
 +
including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective,
 +
inexpensive sensor for arsenic.</span></span></span></p>
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<h2 id="vbid-6e43f075-1nfzkid3" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
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<h2 id="vbid-b282ff56-jo5dppjh" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" style="color: black">Our Project<br></h2>
 
 
 
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;background-color:#ffffff;"><span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span><span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><br></p>
 +
<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.</span></span></span></p>
 +
<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><br></p>
 +
<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.</span></span></p>
 +
<span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span>
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<h3 id="vbid-6e43f075-qeaekp15" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
 
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<span id="vbid-6e43f075-9lechpq7"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
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<div class="preview-subtitle-holder removable-parent order-handle">
 
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<h3 id="element-1b60f7ad57662d8" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">"Cadets2Vets is a team that unites military and civilian resources to investigate new ways of detecting toxic arsenic contamination in the environment. Our team is a community lab made up of students from the U.S. Military Academy at West Point, high school, and undergraduate students from schools in the  Puget Sound area. We are using synthetic biology to develop a gene circuit that detects arsenic ions and then produces a reporter protein in response. The arsenic sensor can be used in military or civilian applications to help inform people of the possible dangers they may encounter while out in the field or at home."<br><br>- PI Dr. David Hirschberg, iGEM 2017<br></h3>
  
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<p><b>Purpose of the Cadets2Vets Team</b><span style="color:#808080;">, iGEM 2017</span></p>
 
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+
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Latest revision as of 00:11, 2 November 2017

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Cadets2Vets


The Issue






As outlined by measure 7061A from the Environmental Protection Agency, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample.


Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions, including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective, inexpensive sensor for arsenic.



Our Project






Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.


These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of E. coli plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.


Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.



"Cadets2Vets is a team that unites military and civilian resources to investigate new ways of detecting toxic arsenic contamination in the environment. Our team is a community lab made up of students from the U.S. Military Academy at West Point, high school, and undergraduate students from schools in the Puget Sound area. We are using synthetic biology to develop a gene circuit that detects arsenic ions and then produces a reporter protein in response. The arsenic sensor can be used in military or civilian applications to help inform people of the possible dangers they may encounter while out in the field or at home."

- PI Dr. David Hirschberg, iGEM 2017


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