Difference between revisions of "Team:DTU-Denmark/Model"
| Line 53: | Line 53: | ||
<p>Lastly, we looked at the fluorescence output in terms of enzymatic activity. We fit a non-linear model in the reactions and calculated their rates. You can find the models for all the substrates <a href="https://static.igem.org/mediawiki/2017/2/2d/T--DTU-Denmark--modelling_all_signal_by_log.pdf">here</a></p><br /> | <p>Lastly, we looked at the fluorescence output in terms of enzymatic activity. We fit a non-linear model in the reactions and calculated their rates. You can find the models for all the substrates <a href="https://static.igem.org/mediawiki/2017/2/2d/T--DTU-Denmark--modelling_all_signal_by_log.pdf">here</a></p><br /> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/5/51/T--DTU-Denmark--modelling_J12_MR.png" alt="J12_MR" width="500"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/3/3b/T--DTU-Denmark--modelling_N21_MR.png" alt="N21_MR" width="500"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/8/8d/T--DTU-Denmark--modelling_B17_MR.png" alt="B17_MR" width="500"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/7/7b/T--DTU-Denmark--modelling_O22_MR.png" alt="O22_MR" width="500"> | ||
| + | <figcaption>Figure 2a, 2b, 2c, 2d: Non-linear model fitted in fluorescence measurements. The sequences of the peptides can be seen next to their well numbers. J12 is cleaved by all three venoms, N21 by <i>Bitis gabonica</i> and <i>Bitis arietans</i>, B17 only by <i>Bitis arietans</i>, and O22 only by <i>Bitis gabonica</i>. Background noise has been deducted from the measurements.</figcaption> | ||
| + | </figure> | ||
| − | + | <figure> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/5/55/T--DTU-Denmark--modelling_histogram.png" alt="Histogram" width="500"> | |
| − | + | <figcaption> Figure 3: Occurrences of enzymatic rates of the models for each snake venom. </figcaption> | |
| − | + | </figure> | |
Revision as of 00:22, 2 November 2017
Substrate Set
Our substrate screening experiment produced a large number of interesting substrates for our detection assay. We looked at the raw fluorescence measurements, but we also applied statistical analysis to identify the peptides that had significant differences between the three venoms. In addition, we modelled the enzymatic activity of the venoms based on our measurements.
You can find the plots of logarithmic fluorescence signal and time of all the substrates here
The relative signal of each substrate between the three snakes was also analyzed. You can find that part here
Lastly, we looked at the fluorescence output in terms of enzymatic activity. We fit a non-linear model in the reactions and calculated their rates. You can find the models for all the substrates here
