LukasPlatz (Talk | contribs) |
LukasPlatz (Talk | contribs) |
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For the characterization of this part, we conducted experiments for the production of organosilicons and therefore used a previously described triple mutant, already engineered by [Arnold et al., 2016]. According to the {{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Experiments">protocol</a>}}, we obtained the matured cytochrome c and performed conversion experiments analyzed via GC-MS method. The following figures show the results obtained with the previously engineered enzyme. | For the characterization of this part, we conducted experiments for the production of organosilicons and therefore used a previously described triple mutant, already engineered by [Arnold et al., 2016]. According to the {{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Experiments">protocol</a>}}, we obtained the matured cytochrome c and performed conversion experiments analyzed via GC-MS method. The following figures show the results obtained with the previously engineered enzyme. | ||
+ | {{Heidelberg/templateus/Contentsection|{{#tag:html| | ||
{{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png|Figure 1:|Compounds used in the project of organosilicons and its respective products.|}} | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png|Figure 1:|Compounds used in the project of organosilicons and its respective products.|}} | ||
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As these results only show activity for a previously engineered cytochrome c, we are convinced that our part is still functional despite not as effective as the mutant. This part as our best basic part is intended as a platform for future teams to evolve their own cytochrome c for their specific target molecule. | As these results only show activity for a previously engineered cytochrome c, we are convinced that our part is still functional despite not as effective as the mutant. This part as our best basic part is intended as a platform for future teams to evolve their own cytochrome c for their specific target molecule. | ||
}} | }} | ||
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Revision as of 00:35, 2 November 2017
BBa_K2398000
We present as best basic part the codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus that is able to convert silicon educts to organosilicon products.![Circularization Construct](https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png)
![](https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png)
Figure 1:
Compounds used in the project of organosilicons and its respective products.
![](https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png)
Figure 2:
Gas chromatogram for the reaction of educt (1) and (3) to the product (5). 11.7 minutes retention time indicates product formation. Unconverted educts converge 6.9 and 7.2 (7.4) minutes.
![](https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png)
Figure 3:
Mass chromatogram shows the breakdown of the product (5). The product itself corresponds to a mass of 251 daltons.
![](https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png)
Figure 4:
Gas chromatogram for the reaction of educt (2) and (3) to the product (4). 9.2 minutes retention time indicates product formation.
![](https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png)
Figure 5:
Mass chromatogram shows the breakdown of the product (4). The product itself corresponds to a mass of 236 daltons.