Difference between revisions of "Team:TUDelft/Main-Parts"
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{{#tag:html| | {{#tag:html| | ||
| − | + | <main class="row"> | |
| − | + | <div class="topper"> | |
| − | <div class=" | + | <img src="https://static.igem.org/mediawiki/2017/f/f4/T--TUDelft--2017_Parts.png"> |
| − | + | </div> | |
| − | + | ||
<div class="col m1"></div> | <div class="col m1"></div> | ||
<div class="col m10 s12" id="parts-page"> | <div class="col m10 s12" id="parts-page"> | ||
| − | < | + | <p> |
| + | When constructing our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts we synthesised contain the coding sequence, RBS, promoter and terminators, if applicable. Therefore, these parts are composite. The different basic parts comprising the composite parts are documented separately, and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. These basic parts were generated by using PCR and restriction ligation on the synthesised composite parts. We created a part collection comprised of 4 composite parts encoding for different Tardigrade Proteins (TDPs) and the 4 basic parts of these genes, all with elaborate characterisation so the user may choose the best protein for their project accordingly. For the composite parts of the TDPs we designed a new, strong RBS. This part does not exist separately. Additionally, we include the documentation of a basic part encoding the gene for Cas13a, derived from the plasmid pC011 from the authors of <a href="#references">Gootenberg et al. 2017</a>. This BioBrick does not exist separately. Finally, we submitted a composite part containing a nonsense spacer with flanking double repeats (with constitutive promoter), appropriate for use for Cas13a. This part is designed so that the user can easily change the spacer according to the sequence they wish to target. Find more info on this cool part on its page in the registry! We also documented this as a basic part without a promoter. This part does not exist separately. Our favourite basic part is <a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a> and our favourite composite part is <a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a>. | ||
| + | |||
| + | </p> | ||
| + | <h3 id="Improved_Part-scroll">Improved BioBrick / Further Characterisation</h3> | ||
| + | <p> | ||
| + | In our project, we added to the characterisation of <a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002 target="_blank">an existing Biobrick</a>, the TolR, periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TorA-GFP composite part was based on the <a href=http://parts.igem.org/Part:BBa_K1082001 target="_blank">BioBrick created by NTU Trontheim</a> with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function. | ||
| + | |||
| + | </p> | ||
<h3>All Parts</h3> | <h3>All Parts</h3> | ||
| Line 13: | Line 20: | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| − | + | <th style=": 15px" colspan="">Fave Part</th> | |
<th style=": 87px;">Name</th> | <th style=": 87px;">Name</th> | ||
<th style=": 80px" colspan="">Type</th> | <th style=": 80px" colspan="">Type</th> | ||
| Line 23: | Line 30: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306000 target="_blank">BBa_K2306000</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>TorA-GFP fusion</td> | <td>TorA-GFP fusion</td> | ||
| Line 31: | Line 38: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306001 target="_blank">BBa_K2306001</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>TorA-GFP fusion (LacI | + | <td>TorA-GFP fusion (LacI promoter)</td> |
<td>Gabriella Tany</td> | <td>Gabriella Tany</td> | ||
<td>1212</td> | <td>1212</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Secretory-abundant heat soluble protein 68234 (SAHS 68234)</td> | <td>Secretory-abundant heat soluble protein 68234 (SAHS 68234)</td> | ||
| Line 47: | Line 54: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td><p>♥</p></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Secretory-abundant heat soluble protein 33020 (SAHS 33020)</td> | <td>Secretory-abundant heat soluble protein 33020 (SAHS 33020)</td> | ||
| Line 55: | Line 62: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)</td> | <td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)</td> | ||
| Line 63: | Line 70: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)</td> | <td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)</td> | ||
| Line 71: | Line 78: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)</td> | <td>Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)</td> | ||
| Line 79: | Line 86: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td>♥</td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)</td> | <td>Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)</td> | ||
| Line 87: | Line 94: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator</td> | <td>Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator</td> | ||
| Line 95: | Line 102: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)</td> | <td>Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)</td> | ||
| Line 103: | Line 110: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306012 target="_blank">BBa_K2306012</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Cas13a </td> | <td>Cas13a </td> | ||
| Line 111: | Line 118: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | + | <td></td> | |
| − | <td><a ="noul_link part_link" href= | + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306013 target="_blank">BBa_K2306013</a></td> |
| + | <td>Composite</td> | ||
| + | <td>Cas13a spacer with flanking double repeats, constitutive promoter</td> | ||
| + | <td>Jeroen Jacques and Jasper Veerman</td> | ||
| + | <td>134</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306014 target="_blank">BBa_K2306014</a></td> | ||
| + | <td>RBS</td> | ||
| + | <td>RBS from bacteriophage T7 gene 10 and Shine Dalgarno</td> | ||
| + | <td>Guillermo Serena Ruiz</td> | ||
| + | <td>30</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | ||
<td>Other</td> | <td>Other</td> | ||
<td>Cas13a spacer with flanking double repeats</td> | <td>Cas13a spacer with flanking double repeats</td> | ||
<td>Jeroen Jacques and Jasper Veerman</td> | <td>Jeroen Jacques and Jasper Veerman</td> | ||
| − | <td> | + | <td>99</td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
| Line 122: | Line 145: | ||
| − | <h3>Basic Parts</h3> | + | <h3 id="Basic_Parts-scroll">Basic Parts</h3> |
<table> | <table> | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th>Fave Part</th> | ||
<th>Name</th> | <th>Name</th> | ||
<th>Type</th> | <th>Type</th> | ||
| Line 135: | Line 159: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> | ||
| + | |||
<td>Basic</td> | <td>Basic</td> | ||
| − | <td>Secretory | + | <td>Secretory-abundant heat soluble protein (SAHS) 68234</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>525</td> | <td>525</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td>♥</td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> | ||
| + | |||
<td>Basic</td> | <td>Basic</td> | ||
| − | <td>Secretory | + | <td>Secretory-abundant heat soluble protein (SAHS) 33020</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>509</td> | <td>509</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| − | <td>Cytosolic | + | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>684</td> | <td>684</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| − | <td>Cytosolic | + | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>681</td> | <td>681</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306000 target="_blank">BBa_K23006000</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
| − | <td> | + | <td>TorA GFP</td> |
<td>Gabriella Tany</td> | <td>Gabriella Tany</td> | ||
<td>681</td> | <td>681</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306012 target="_blank">BBa_K2306012</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>Cas13a</td> | <td>Cas13a</td> | ||
| Line 176: | Line 208: | ||
<td>3456</td> | <td>3456</td> | ||
</tr> | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | |||
| + | <td><a ="noul_link part_link" href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306014 target="_blank">BBa_K2306014</a></td> | ||
| + | <td>RBS</td> | ||
| + | <td>RBS from bacteriophage T7 gene 10 and Shine Dalgarno</td> | ||
| + | <td>Guillermo Serena Ruiz</td> | ||
| + | <td>30</td> | ||
| + | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>Spacer with flanking double repeats for Cas13a</td> | <td>Spacer with flanking double repeats for Cas13a</td> | ||
| − | <td>Jeroen Jacques, | + | <td>Jeroen Jacques, Jasper Veerman</td> |
| − | <td> | + | <td>99</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | <h3 id=" | + | <h3 id="Composite_Parts-scroll">Composite Parts</h3> |
<table> | <table> | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th>Fave Part</th> | ||
<th>Name</th> | <th>Name</th> | ||
<th>Type</th> | <th>Type</th> | ||
| Line 196: | Line 239: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Secretory | + | <td>Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>833</td> | <td>833</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td>♥</td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> | ||
| + | |||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Secretory | + | <td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>817</td> | <td>817</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Cytosolic | + | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>992</td> | <td>992</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Cytosolic | + | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>989</td> | <td>989</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306001 target="_blank">BBa_K2306001</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td> | + | <td>TorA GFP fusion with inducible LacI promoter, RBS and double terminator</td> |
<td>Gabriella Tany</td> | <td>Gabriella Tany</td> | ||
<td>681</td> | <td>681</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306015 target="_blank">BBa_K2306015</a></td> | ||
| + | <td>Composite</td> | ||
| + | <td>Cas13a spacer with flanking double repeats, constitutive promoter</td> | ||
| + | <td>Jeroen Jacques, Jasper Veerman</td> | ||
| + | <td>134</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | <h3 id="Part_Collection">Part Collection</h3> | + | <h3 id="Part_Collection-scroll">Part Collection</h3> |
<table> | <table> | ||
<thead> | <thead> | ||
<tr> | <tr> | ||
| + | <th>Fave Part</th> | ||
<th>Name</th> | <th>Name</th> | ||
<th>Type</th> | <th>Type</th> | ||
| Line 244: | Line 302: | ||
</thead> | </thead> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306007 target="_blank">BBa_K2306007</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Secretory | + | <td>Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>833</td> | <td>833</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td>♥</td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306008 target="_blank">BBa_K2306008</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Secretory | + | <td>Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>817</td> | <td>817</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306010 target="_blank">BBa_K2306010</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Cytosolic | + | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>992</td> | <td>992</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href= | + | <td></td> |
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306011 target="_blank">BBa_K2306011</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Cytosolic | + | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator</td> |
<td>Guillermo Serena Ruiz</td> | <td>Guillermo Serena Ruiz</td> | ||
<td>989</td> | <td>989</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306002 target="_blank">BBa_K2306002</a></td> | ||
| + | <td>Basic</td> | ||
| + | <td>Secretory-abundant heat soluble protein (SAHS) 68234</td> | ||
| + | <td>Guillermo Serena Ruiz</td> | ||
| + | <td>525</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>♥</td> | ||
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306003 target="_blank">BBa_K2306003</a></td> | ||
| + | |||
| + | <td>Basic</td> | ||
| + | <td>Secretory-abundant heat soluble protein (SAHS) 33020</td> | ||
| + | <td>Guillermo Serena Ruiz</td> | ||
| + | <td>509</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306005 target="_blank">BBa_K2306005</a></td> | ||
| + | <td>Basic</td> | ||
| + | <td>Cytosolic-abundant heat soluble protein (CAHS) 106094</td> | ||
| + | <td>Guillermo Serena Ruiz</td> | ||
| + | <td>684</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2306006 target="_blank">BBa_K2306006</a></td> | ||
| + | <td>Basic</td> | ||
| + | <td>Cytosolic-abundant heat soluble protein (CAHS) 94205</td> | ||
| + | <td>Guillermo Serena Ruiz</td> | ||
| + | <td>681</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | ||
| − | + | </div></div> | |
| − | + | ||
| − | + | <div id="references" class="section card"> | |
| + | <div class='card-content'> | ||
| + | <span class='card-title'>References:</span> | ||
| + | <ol> | ||
| + | <li><a href="http://www.sciencemag.org/lookup/doi/10.1126/science.aam9321" target="_blank">Gootenberg, J.S. et al., 2017. Nucleic acid detection with CRISPR-Cas13a/C2c2. Science, 356(6336), pp.438–442.</a></li> | ||
| + | </ol> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="col m1"></div> | ||
| + | |||
</main> | </main> | ||
}} | }} | ||
Latest revision as of 00:39, 2 November 2017
When constructing our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts we synthesised contain the coding sequence, RBS, promoter and terminators, if applicable. Therefore, these parts are composite. The different basic parts comprising the composite parts are documented separately, and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. These basic parts were generated by using PCR and restriction ligation on the synthesised composite parts. We created a part collection comprised of 4 composite parts encoding for different Tardigrade Proteins (TDPs) and the 4 basic parts of these genes, all with elaborate characterisation so the user may choose the best protein for their project accordingly. For the composite parts of the TDPs we designed a new, strong RBS. This part does not exist separately. Additionally, we include the documentation of a basic part encoding the gene for Cas13a, derived from the plasmid pC011 from the authors of Gootenberg et al. 2017. This BioBrick does not exist separately. Finally, we submitted a composite part containing a nonsense spacer with flanking double repeats (with constitutive promoter), appropriate for use for Cas13a. This part is designed so that the user can easily change the spacer according to the sequence they wish to target. Find more info on this cool part on its page in the registry! We also documented this as a basic part without a promoter. This part does not exist separately. Our favourite basic part is BBa_K2306003 and our favourite composite part is BBa_K2306008.
In our project, we added to the characterisation of an existing Biobrick, the TolR, periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TorA-GFP composite part was based on the BioBrick created by NTU Trontheim with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.
♥
Improved BioBrick / Further Characterisation
All Parts
Fave Part
Name
Type
Description
Designer
Length
BBa_K2306000
Coding
TorA-GFP fusion
Gabriella Tany
846
BBa_K2306001
Composite
TorA-GFP fusion (LacI promoter)
Gabriella Tany
1212
BBa_K2306002
Coding
Secretory-abundant heat soluble protein 68234 (SAHS 68234)
Guillermo Serena Ruiz
525
BBa_K2306003
Coding
Secretory-abundant heat soluble protein 33020 (SAHS 33020)
Guillermo Serena Ruiz
509
BBa_K2306005
Coding
Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)
Guillermo Serena Ruiz
684
BBa_K2306006
Coding
Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)
Guillermo Serena Ruiz
681
BBa_K2306007
Composite
Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
768
♥
BBa_K2306008
Composite
Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
752
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
927
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)
Guillermo Serena Ruiz
924
BBa_K2306012
Coding
Cas13a
Jeroen Jacques
3920
BBa_K2306013
Composite
Cas13a spacer with flanking double repeats, constitutive promoter
Jeroen Jacques and Jasper Veerman
134
BBa_K2306014
RBS
RBS from bacteriophage T7 gene 10 and Shine Dalgarno
Guillermo Serena Ruiz
30
BBa_K2306015
Other
Cas13a spacer with flanking double repeats
Jeroen Jacques and Jasper Veerman
99
Basic Parts
Fave Part
Name
Type
Description
Designer
Size (bp)
BBa_K2306002
Basic
Secretory-abundant heat soluble protein (SAHS) 68234
Guillermo Serena Ruiz
525
♥
BBa_K2306003
Basic
Secretory-abundant heat soluble protein (SAHS) 33020
Guillermo Serena Ruiz
509
BBa_K2306005
Basic
Cytosolic-abundant heat soluble protein (CAHS) 106094
Guillermo Serena Ruiz
684
BBa_K2306006
Basic
Cytosolic-abundant heat soluble protein (CAHS) 94205
Guillermo Serena Ruiz
681
BBa_K23006000
Basic
TorA GFP
Gabriella Tany
681
BBa_K2306012
Basic
Cas13a
Jeroen Jacques
3456
BBa_K2306014
RBS
RBS from bacteriophage T7 gene 10 and Shine Dalgarno
Guillermo Serena Ruiz
30
BBa_K2306015
Basic
Spacer with flanking double repeats for Cas13a
Jeroen Jacques, Jasper Veerman
99
Composite Parts
Fave Part
Name
Type
Description
Designer
Size (bp)
BBa_K2306007
Composite
Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
833
♥
BBa_K2306008
Composite
Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
817
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
992
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
989
BBa_K2306001
Composite
TorA GFP fusion with inducible LacI promoter, RBS and double terminator
Gabriella Tany
681
BBa_K2306015
Composite
Cas13a spacer with flanking double repeats, constitutive promoter
Jeroen Jacques, Jasper Veerman
134
Part Collection
Fave Part
Name
Type
Description
Designer
Size (bp)
BBa_K2306007
Composite
Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
833
♥
BBa_K2306008
Composite
Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
817
BBa_K2306010
Composite
Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
992
BBa_K2306011
Composite
Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator
Guillermo Serena Ruiz
989
BBa_K2306002
Basic
Secretory-abundant heat soluble protein (SAHS) 68234
Guillermo Serena Ruiz
525
♥
BBa_K2306003
Basic
Secretory-abundant heat soluble protein (SAHS) 33020
Guillermo Serena Ruiz
509
BBa_K2306005
Basic
Cytosolic-abundant heat soluble protein (CAHS) 106094
Guillermo Serena Ruiz
684
BBa_K2306006
Basic
Cytosolic-abundant heat soluble protein (CAHS) 94205
Guillermo Serena Ruiz
681
