Difference between revisions of "Team:SDU CHINA/InterLab"

 
(21 intermediate revisions by the same user not shown)
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<style>
 
<style>
 
.top{text-align:center;margin-top:2em}
 
.top{text-align:center;margin-top:2em}
−
.p{text-indent:2em;word-wrap:break-word;font-size:1.1em;word-spacing:0.2em}
+
.p{text-indent:2em;word-wrap:break-word;font-size:1.2em;word-space:0.08em;letter-spacing:0.06em;}
 
.tu{text-align:center;font-size:1em}
 
.tu{text-align:center;font-size:1em}
−
.all{margin:0 10% auto 10%;font-family: Segoe, "Segoe UI", "DejaVu Sans", "Trebuchet MS", Verdana, "sans-serif";letter-spacing:0.05em}
+
.all{margin:0 20% auto 20%;font-family:"Times New Roman", Times, serif;;letter-spacing:0.04em;word-spacing:0.06em}
 
.title{font-size:1.6em;text-align:center;font-family:Constantia, "Lucida Bright", "DejaVu Serif", Georgia, "serif"}
 
.title{font-size:1.6em;text-align:center;font-family:Constantia, "Lucida Bright", "DejaVu Serif", Georgia, "serif"}
−
.subtitle{font-size:1.3em;font-family: Baskerville, "Palatino Linotype", Palatino, "Century Schoolbook L", "Times New Roman", "serif"}
+
.subtitle{font-size:1.3em;font-family: "Times New Roman", Times, serif;}
 +
#HQ_page p {
 +
    font-family: "Times New Roman", Times, serif;
 +
    font-size: 1.2em;
 +
    text-align: justify;
 +
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</style>
 
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</head>
 
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<div class="p">
 
<div class="p">
 
<strong style="color:#05BAC3">>BBa_I20270 Part-only sequence (919 bp)</strong>
 
<strong style="color:#05BAC3">>BBa_I20270 Part-only sequence (919 bp)</strong>
−
<p style="textindent:0;">
+
<p style="text-indent:0;">
−
ttgatggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtccc
+
ttgatggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac
−
aattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattt
+
tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br>
−
atttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
+
−
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaa
+
−
gtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac
+
−
tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
+
−
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacga
+
−
aaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
+
−
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
+
−
gggcctttctgcgtttata<br><br>
+
 
</p></div>
 
</p></div>
  
 
<div class="p">
 
<div class="p">
−
<p style="textindent:0;">
+
<strong style="color:#05BAC3">>BBa_R0040 Part-only sequence (154 bp)</strong>
 +
<p style="text-indent:0;">
 +
 
 
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac<br><br>
 
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac<br><br>
 
</p></div>
 
</p></div>
  
 
<div class="p">
 
<div class="p">
−
<p style="textindent:0;">
+
<strong style="color:#05BAC3">>BBa_J364000 Part-only sequence (918 bp)</strong>
−
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
+
<p style="text-indent:0;">
−
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
+
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcaccaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
−
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
+
−
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
+
−
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
+
−
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
+
−
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
+
−
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
−
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
+
−
ggcctttctgcgtttata<br><br></p>
+
 
</div>
 
</div>
  
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<div class="p">
 
<div class="p">
 
<strong style="color:#05BAC3">>BBa_J364001 Part-only sequence (918 bp)</strong>
 
<strong style="color:#05BAC3">>BBa_J364001 Part-only sequence (918 bp)</strong>
−
<p style="textindent:0;">
+
<p style="text-indent:0;">
−
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
+
 
−
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
+
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
−
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
+
−
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
+
−
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
+
−
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
+
−
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
+
−
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
−
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
+
−
ggcctttctgcgtttata<br><br></p>
+
 
</div>
 
</div>
  
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<div class="p">
 
<div class="p">
 
<strong style="color:#05BAC3">>BBa_J364002 Part-only sequence (918 bp)</strong>
 
<strong style="color:#05BAC3">>BBa_J364002 Part-only sequence (918 bp)</strong>
−
<p style="textindent:0;">
+
<p style="text-indent:0;">
−
ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
+
 
−
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
+
ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcccacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
−
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
+
−
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
+
−
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
+
−
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
+
−
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
+
−
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
−
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
+
−
ggcctttctgcgtttata<br><br></p>
+
 
</div>
 
</div>
  
 
<div class="p">
 
<div class="p">
 
<strong style="color:#05BAC3">>BBa_J364003 Part-only sequence (990 bp)</strong>
 
<strong style="color:#05BAC3">>BBa_J364003 Part-only sequence (990 bp)</strong>
−
<p style="textindent:0;">
+
<p style="text-indent:0;">
−
tttacagctagctcagtcctaggtattatgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaac
+
 
−
atcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtta
+
tttacagctagctcagtcctaggtattatgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
−
atgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
+
−
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgccc
+
−
gaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
+
−
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaa
+
−
acaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
+
−
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
+
−
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
+
−
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
+
 
</div>
 
</div>
  
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<div class="p">
 
<div class="p">
 
<strong style="color:#05BAC3">>BBa_J364004 Part-only sequence (990 bp)</strong>
 
<strong style="color:#05BAC3">>BBa_J364004 Part-only sequence (990 bp)</strong>
−
<p style="textindent:0;">
+
<p style="text-indent:0;">
−
tttacggctagctcagtcctaggtatagtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaac
+
tttacggctagctcagtcctaggtatagtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcggttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
−
atcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtta
+
−
atgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
+
−
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgccc
+
−
gaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
+
−
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaa
+
−
acaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
+
−
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
+
−
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
+
−
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
+
 
</div>
 
</div>
  
Line 220: Line 180:
 
<div class="p">
 
<div class="p">
 
<strong style="color:#05BAC3">>BBa_J364005 Part-only sequence (990 bp)</strong>
 
<strong style="color:#05BAC3">>BBa_J364005 Part-only sequence (990 bp)</strong>
−
<p style="textindent:0;">
+
<p style="text-indent:0;">
−
ttgacagctagctcagtcctagggattgtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaac
+
 
−
atcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtta
+
ttgacagctagctcagtcctagggattgtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
−
atgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
+
−
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgccc
+
−
gaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
+
−
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaa
+
−
acaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
+
−
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
+
−
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
+
−
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata<br><br></p>
+
 
</div>
 
</div>
 
</div>
 
</div>
Line 243: Line 195:
 
<div class="subtitle" style="text-align:center"><strong style="color:#D5292C">OD600 Reference Point</strong></div>
 
<div class="subtitle" style="text-align:center"><strong style="color:#D5292C">OD600 Reference Point</strong></div>
 
<br>
 
<br>
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/f/fb/Interlab_table1.png" style="width:600px;margin:0 auto"></p>
+
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/f/fb/Interlab_table1.png" style="width:80%;margin:0 auto"></p>
 
<div class="tu">
 
<div class="tu">
 
   <p  style="text-align:center"><strong>Table 1</strong>. OD600 Reference Point.</p>
 
   <p  style="text-align:center"><strong>Table 1</strong>. OD600 Reference Point.</p>
Line 252: Line 204:
 
<div class="subtitle"  style="text-align:center"><strong style="color:#D5292C">Fluorescein Standard Curve</strong></div>
 
<div class="subtitle"  style="text-align:center"><strong style="color:#D5292C">Fluorescein Standard Curve</strong></div>
 
<br>
 
<br>
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/5/5d/Interlab_table2.png" style="width:100%;margin:0 auto"></p>
+
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/5/5d/Interlab_table2.png" style="width:90%;margin:0 auto"></p>
−
<br>
+
 
<div class="tu"  style="text-align:center"><strong>Table 2. </strong>Data of Fluorescein Standard Curve.</div>
 
<div class="tu"  style="text-align:center"><strong>Table 2. </strong>Data of Fluorescein Standard Curve.</div>
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/6/6b/Interlab_figure1.png" style="width:400px;margin:0 auto"></p>
 
 
<br>
 
<br>
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/6/6b/Interlab_figure1.png" style="width:80%;margin:0 auto"></p>
 
<div class="tu"  style="text-align:center"><strong>Figure 1</strong></div>
 
<div class="tu"  style="text-align:center"><strong>Figure 1</strong></div>
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/0/00/Interlab_figure2.png" style="width:400px;margin:0 auto"></p>
 
 
<br>
 
<br>
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/0/00/Interlab_figure2.png" style="width:80%;margin:0 auto"></p>
 
<div class="tu"  style="text-align:center">
 
<div class="tu"  style="text-align:center">
 
   <p  style="text-align:center" ><strong>Figure 2</strong></p>
 
   <p  style="text-align:center" ><strong>Figure 2</strong></p>
 +
<br>
 
   <p><br><br>
 
   <p><br><br>
 
</p></div>
 
</p></div>
Line 273: Line 225:
 
<div class="subtitle"  style="text-align:center" ><strong style="color:#D5292C">Cell Measurement</strong></div>
 
<div class="subtitle"  style="text-align:center" ><strong style="color:#D5292C">Cell Measurement</strong></div>
 
<br><br>
 
<br><br>
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/d/da/Interlab_table4.png" width="100%"></p>
+
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/d/da/Interlab_table4.png" width="110%"></p>
−
<p></p>
+
<div class="tu"  style="text-align:center"><strong>Table 4.</strong> Raw data of OD600 measurement.</div>
−
<div class="tu"  style="text-align:center"><strong>Table 4.</strong> Raw data of OD600 measurement.</div>
+
−
 
+
 
   <p>&nbsp;</p>
 
   <p>&nbsp;</p>
 
</div>
 
</div>
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/c/c7/Interlab_table5.png"width="100%"></p>
+
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/c/c7/Interlab_table5.png"width="110%"></p>
 
  <div class="tu"  style="text-align:center">
 
  <div class="tu"  style="text-align:center">
 
   <p  style="text-align:center"><strong>Table 5. </strong>Blank subtraction and correction of OD600 measurement.</p>
 
   <p  style="text-align:center"><strong>Table 5. </strong>Blank subtraction and correction of OD600 measurement.</p>
Line 285: Line 236:
 
  </div>
 
  </div>
  
−
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/b/
+
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/b/b5/Interlab_figure3.png" style="width:80%;margin:0 auto"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Figure 3. </strong>Blank subtraction and correction of Abs600 measurement.</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/8/87/Interlab_table6.png"width="110%"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Table 6. </strong>Raw data of fluorescence measurement.</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/b/bc/Interlab_table7.png" width="110%"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Table 7. </strong>Blank substraction and correction of fluorescence measurement.</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/2/20/Interlab_figure4.png" style="width:60%;margin:0 auto"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Figure 4. </strong>Blank subtraction and correction of fluorescence measurement.</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/7/7b/Interlab_table8.png"width="110%"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Table 8.</strong>Raw data of Fl/Abs600.</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/2/2e/Interlab_table9.png"width="80%"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Table 9. </strong>Raw data of average</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/1/17/Interlab_table10.png"width="80%"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Table 10. </strong>Raw data of SD</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
<p style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/2/24/Interlab_figure5.png" style="width:80%;margin:0 auto"></p>
 +
<div class="tu"  style="text-align:center">
 +
  <p  style="text-align:center"><strong>Figure 5. </strong>Average level of devices.</p>
 +
  <p>&nbsp;</p>
 +
</div>
 +
 
 +
</div>
 +
<br><br>
 +
<div class="footest"><img src="https://static.igem.org/mediawiki/2017/c/c0/Foot.jpeg" style="width:100%;height:auto"></div>

Latest revision as of 00:41, 2 November 2017



Background

 

“All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which aimed to be one of the labs to repeat measurements in different condition. In our case, we measured fluorescence using plate reader.




Design

 

We often used fluorescence as an indicator of promoter activity. However, the ability to repeat measurements in different labs has been difficult. To solve this problem, InterLab study has been developed as a robust measurement procedure for green fluorescent protein (GFP) which is one of the most used markers in synthetic biology.
This year's iGEM Interlab study is to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable and compare the results from various teams. iGEM Interlab study has provided 2 protocols of measuring GFP, and we choose “plate reader”. We have to measure absorbance at 600 nm and GFP fluorescence for GFP expression per cell for all devices.




Materials


Plasmids used
  • Positive Control Device: I20270 in pSB1C3 (Located in Kit Plate 6, well 20B)
  • Negative Control Device: R0040 in pSB1C3 (Located in Kit Plate 6, well 20D)
  • Test Device 1: J23101+I13504 in pSB1C3 (Located in Kit Plate 6, well 20F)
  • Test Device 2: J23106+I13504 in pSB1C3 (Located in Kit Plate 6, well 20H)
  • Test Device 3: J23117+I13504 in pSB1C3 (Located in Kit Plate 6, well 20J)
  • Test Device 4: J23101.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20L)
  • Test Device 5: J23106.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20N)
  • Test Device 6: J23117.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20P)


Plasmids used
  • Escherichia coli​ strain DH5α
  • Materials
  • 1ml LUDOX
  • H​2​0
  • 96 well plate
  • fluorescein
  • 10ml 1xPBS
  • LB (Luria Bertani) media
  • Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)
  • 50 ml Falcon tube
  • Incubator at 37°C
  • 1.5 ml eppendorf tubes
  • Ice bucket with ice
  • Pipettes


Plasmids used
  • Thermo Multiskan FC
  • BERTHOLD TECHNOLOGIES LB940Mith








Results


Sequencing
  • Positive Control ：BBa_I20270
  • Negative Control ：BBa_R0040
  • Test Device 1 ：BBa_J364000
  • Test Device 2 ：BBa_J364001
  • Test Device 3 ：BBa_J364002
  • Test Device 4 ：BBa_J364003
  • Test Device 5 ：BBa_J364004
  • Test Device 6 ：BBa_J364005


>BBa_I20270 Part-only sequence (919 bp)

ttgatggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

>BBa_R0040 Part-only sequence (154 bp)

tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac

>BBa_J364000 Part-only sequence (918 bp)

tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcaccaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

>BBa_J364001 Part-only sequence (918 bp)

tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

>BBa_J364002 Part-only sequence (918 bp)

ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcccacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

>BBa_J364003 Part-only sequence (990 bp)

tttacagctagctcagtcctaggtattatgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

>BBa_J364004 Part-only sequence (990 bp)

tttacggctagctcagtcctaggtatagtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcggttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

>BBa_J364005 Part-only sequence (990 bp)

ttgacagctagctcagtcctagggattgtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata





Data


OD600 Reference Point

Table 1. OD600 Reference Point.

 



Fluorescein Standard Curve

Table 2. Data of Fluorescein Standard Curve.

Figure 1

Figure 2




Normalization


Table 3. Normalization.

 

Cell Measurement


Table 4. Raw data of OD600 measurement.

 

Table 5. Blank subtraction and correction of OD600 measurement.

 

Figure 3. Blank subtraction and correction of Abs600 measurement.

 

Table 6. Raw data of fluorescence measurement.

 

Table 7. Blank substraction and correction of fluorescence measurement.

 

Figure 4. Blank subtraction and correction of fluorescence measurement.

 

Table 8.Raw data of Fl/Abs600.

 

Table 9. Raw data of average

 

Table 10. Raw data of SD

 

Figure 5. Average level of devices.