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Furthermore, we performed experiments on the compound (1) which differs from compound (2) in its amino functional group. The product is eluted after 11.7 minutes retention time (Fig. 4) and also confirmed as the right product via mass spectrometry in Fig. 5. Worth mentioning, there is still educt (1) present which emerges at 6.9 minutes retention time, indicating incomplete conversion. Calculations showed a conversion rate of 47.5% for the product (4). | Furthermore, we performed experiments on the compound (1) which differs from compound (2) in its amino functional group. The product is eluted after 11.7 minutes retention time (Fig. 4) and also confirmed as the right product via mass spectrometry in Fig. 5. Worth mentioning, there is still educt (1) present which emerges at 6.9 minutes retention time, indicating incomplete conversion. Calculations showed a conversion rate of 47.5% for the product (4). | ||
− | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png|Figure 4:|Gas chromatogram for the reaction of educt (2) and (3) to the product (4). 9.2 minutes retention time indicates product formation.|}} | + | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png|Figure 4:|Gas chromatogram for the reaction of educt (2) and (3) to the product (4). 9.2 minutes retention time indicates product formation.|style = float:none}} |
{{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png|Figure 5:|Mass chromatogram shows the breakdown of the product (4). The product itself corresponds to a mass of 236 daltons.|style = float:none}} | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png|Figure 5:|Mass chromatogram shows the breakdown of the product (4). The product itself corresponds to a mass of 236 daltons.|style = float:none}} |
Revision as of 00:44, 2 November 2017
BBa_K2398000
We present as best basic part the codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus that is able to convert silicon educts to organosilicon products.![Circularization Construct](https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png)
![](https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png)
Figure 1:
Compounds used in the project of organosilicons and its respective products.
![](https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png)
Figure 2:
Gas chromatogram for the reaction of educt (1) and (3) to the product (5). 11.7 minutes retention time indicates product formation. Unconverted educts converge 6.9 and 7.2 (7.4) minutes.
![](https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png)
Figure 3:
Mass chromatogram shows the breakdown of the product (5). The product itself corresponds to a mass of 251 daltons.
![](https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png)
Figure 4:
Gas chromatogram for the reaction of educt (2) and (3) to the product (4). 9.2 minutes retention time indicates product formation.
![](https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png)
Figure 5:
Mass chromatogram shows the breakdown of the product (4). The product itself corresponds to a mass of 236 daltons.