Difference between revisions of "Team:DTU-Denmark/Parts"

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{{DTU-Denmark}}
 
 
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<h1>Parts</h1>
 
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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          <h1 class="bottomborder">Parts</h1>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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          <p style="text-align:center;">The following table is a summary of the different parts created in the project</p>
  
  
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<figure>
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                    <table>
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                        <tr>
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                            <th>Part Number</th>
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                            <th>Type</th>
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                            <th>Description</th>
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                            <th>Designer</th>
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                        </tr>
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                        <tr>
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                            <td style="width:15%">BBa_K2355001</td>
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                            <td style="width:15%">Protein-domain</td>
 +
                            <td style="width:50%">Peptide linker that is cleaved by <i>Bitis arietans</i> and <i>Bitis gabonica</i> venom.
 +
When translated, this linker should contain 5 x repeats of the ALK motive identified by our experiments to be cleavable by the venom of <i>Bitis sp.</i></td>
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                            <td style="width:20%">Philip Hau Soerensen</td>
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                        </tr>
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                        <tr>
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                            <td>BBa_K2355002</td>
 +
                            <td>Coding</td>
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                            <td>This is a modification of the amilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein</td>
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                            <td>Philip Hau Soerensen</td>
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                        </tr>
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                        <tr>
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                            <td>BBa_K2355006</td>
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                            <td>Protein-domain</td>
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                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i> and <i>Bitis arietans</i>. The specific peptide sequence of the linker is AAGVAPLS</td>
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                            <td>Chrysillis Magaard Polhaus</td>
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                        </tr>
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                        <tr>
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                            <td>BBa_K2355008</td>
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                            <td>Protein-domain</td>
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                            <td>This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. </td>
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                            <td>Cathrine Agnete Larsen </td>
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                        </tr>
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                        <tr>
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                            <td>BBa_K2355014</td>
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                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i> and <i>Bitis arietans</i>. The specific peptide sequence of the linker is PEDDGYFV.</td>
 +
                            <td>Chrysillis Magaard Polhaus</td>
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                        </tr>
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                        <tr>
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                            <td>BBa_K2355015</td>
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                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is LDEDEEDL.</td>
 +
                            <td>Cathrine Agnete Larsen </td>
 +
                        </tr>
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                        <tr>
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                            <td>BBa_K2355025</td>
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                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is DEMDEKSE.</td>
 +
                            <td>Chrysillis Magaard Polhaus</td>
 +
                        </tr>
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                        <tr>
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                            <td>BBa_K2355026</td>
 +
                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. The specific peptide sequence of the linker is PAPDAPLK</td>
 +
                            <td>Cathrine Agnete Larsen </td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>BBa_K2355032</td>
 +
                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. The specific peptide sequence of the linker is RKRRAVLT.</td>
 +
                            <td>Chrysillis Magaard Polhaus</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>BBa_K2355033</td>
 +
                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i> and <i>Bitis arietans</i>. The specific peptide sequence of the linker is DDSDAATF.</td>
 +
                            <td>Cathrine Agnete Larsen </td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>BBa_K2355037</td>
 +
                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>. The specific peptide sequence of the linker is GGVRGPRV.</td>
 +
                            <td>Chrysillis Magaard Polhaus</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td>BBa_K2355039</td>
 +
                            <td>Protein-domain</td>
 +
                            <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is IDIRGPRL.</td>
 +
                            <td>Cathrine Agnete Larsen </td>
 +
                        </tr>
 +
                        <tr>
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                            <td>BBa_K2355302</td>
 +
                            <td>Composite part</td>
 +
                            <td>This part contains two major protein domains: A single chain avidin domain to bind the device to biotinylated surfaces, and amilCP with his-tag. Between the two domains, NgoMIV and AgeI restriction sites are present, to allow for insertion of RFC[25] part. </td>
 +
                            <td>Cathrine Agnete Larsen </td>
 +
                        </tr>
 +
                        <tr>
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                            <td>BBa_K2355313</td>
 +
                            <td>Composite part</td>
 +
                            <td>This part is used for detection of snake venom. The Beta-galactosidase, encoded by lacZ, should allow for an decrease in detection time.</td>
 +
                            <td>Philip Hau Soerensen</td>
 +
                        </tr>
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                    </table>
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 +
                    <figcaption>Table 1: List of all parts</figcaption>
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                </figure>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<div class="rightnavbar rmvpadmarg">
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
 
<ul>
 
<ul>
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
  
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<a href="#"></a>
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<h5>Inspiration</h5>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
 
</ul>
 
</ul>
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</div>
  
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<h5>Part Table </h5>
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    </div>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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                    <p>FIND US AT</p>
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                    <span>DTU BIOBUILDERS</span><br />
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                    <span>DENMARK</span><br />
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                    <span>DTU - SØLTOFTS PLADS, BYGN. 221/006</span><br />
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                    <span>2800 KGS. LYNGBY</span><br />
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<groupparts>iGEM17 DTU-Denmark</groupparts>
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Latest revision as of 01:04, 2 November 2017

Parts

The following table is a summary of the different parts created in the project

Part Number Type Description Designer
BBa_K2355001 Protein-domain Peptide linker that is cleaved by Bitis arietans and Bitis gabonica venom. When translated, this linker should contain 5 x repeats of the ALK motive identified by our experiments to be cleavable by the venom of Bitis sp. Philip Hau Soerensen
BBa_K2355002 Coding This is a modification of the amilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein Philip Hau Soerensen
BBa_K2355006 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS Chrysillis Magaard Polhaus
BBa_K2355008 Protein-domain This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. Cathrine Agnete Larsen
BBa_K2355014 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is PEDDGYFV. Chrysillis Magaard Polhaus
BBa_K2355015 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL. Cathrine Agnete Larsen
BBa_K2355025 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is DEMDEKSE. Chrysillis Magaard Polhaus
BBa_K2355026 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK Cathrine Agnete Larsen
BBa_K2355032 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is RKRRAVLT. Chrysillis Magaard Polhaus
BBa_K2355033 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is DDSDAATF. Cathrine Agnete Larsen
BBa_K2355037 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV. Chrysillis Magaard Polhaus
BBa_K2355039 Protein-domain This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL. Cathrine Agnete Larsen
BBa_K2355302 Composite part This part contains two major protein domains: A single chain avidin domain to bind the device to biotinylated surfaces, and amilCP with his-tag. Between the two domains, NgoMIV and AgeI restriction sites are present, to allow for insertion of RFC[25] part. Cathrine Agnete Larsen
BBa_K2355313 Composite part This part is used for detection of snake venom. The Beta-galactosidase, encoded by lacZ, should allow for an decrease in detection time. Philip Hau Soerensen
Table 1: List of all parts

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