Difference between revisions of "Team:DTU-Denmark/Parts"
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<td>BBa_K2355002</td> | <td>BBa_K2355002</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>This is a modification of the | + | <td>This is a modification of the amilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein</td> |
<td>Philip Hau Soerensen</td> | <td>Philip Hau Soerensen</td> | ||
</tr> | </tr> | ||
| Line 66: | Line 66: | ||
<td>BBa_K2355006</td> | <td>BBa_K2355006</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i> and <i>Bitis arietans</i>. The specific peptide sequence of the linker is AAGVAPLS</td> |
<td>Chrysillis Magaard Polhaus</td> | <td>Chrysillis Magaard Polhaus</td> | ||
</tr> | </tr> | ||
| Line 72: | Line 72: | ||
<td>BBa_K2355008</td> | <td>BBa_K2355008</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. </td> | + | <td>This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. </td> |
<td>Cathrine Agnete Larsen </td> | <td>Cathrine Agnete Larsen </td> | ||
</tr> | </tr> | ||
| Line 78: | Line 78: | ||
<td>BBa_K2355014</td> | <td>BBa_K2355014</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is PEDDGYFV.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i> and <i>Bitis arietans</i>. The specific peptide sequence of the linker is PEDDGYFV.</td> |
<td>Chrysillis Magaard Polhaus</td> | <td>Chrysillis Magaard Polhaus</td> | ||
</tr> | </tr> | ||
| Line 84: | Line 84: | ||
<td>BBa_K2355015</td> | <td>BBa_K2355015</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is LDEDEEDL.</td> |
<td>Cathrine Agnete Larsen </td> | <td>Cathrine Agnete Larsen </td> | ||
</tr> | </tr> | ||
| Line 90: | Line 90: | ||
<td>BBa_K2355025</td> | <td>BBa_K2355025</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is DEMDEKSE.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is DEMDEKSE.</td> |
<td>Chrysillis Magaard Polhaus</td> | <td>Chrysillis Magaard Polhaus</td> | ||
</tr> | </tr> | ||
| Line 96: | Line 96: | ||
<td>BBa_K2355026</td> | <td>BBa_K2355026</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. The specific peptide sequence of the linker is PAPDAPLK</td> |
<td>Cathrine Agnete Larsen </td> | <td>Cathrine Agnete Larsen </td> | ||
</tr> | </tr> | ||
| Line 102: | Line 102: | ||
<td>BBa_K2355032</td> | <td>BBa_K2355032</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is RKRRAVLT.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis arietans</i>. The specific peptide sequence of the linker is RKRRAVLT.</td> |
<td>Chrysillis Magaard Polhaus</td> | <td>Chrysillis Magaard Polhaus</td> | ||
</tr> | </tr> | ||
| Line 108: | Line 108: | ||
<td>BBa_K2355033</td> | <td>BBa_K2355033</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is DDSDAATF.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i> and <i>Bitis arietans</i>. The specific peptide sequence of the linker is DDSDAATF.</td> |
<td>Cathrine Agnete Larsen </td> | <td>Cathrine Agnete Larsen </td> | ||
</tr> | </tr> | ||
| Line 114: | Line 114: | ||
<td>BBa_K2355037</td> | <td>BBa_K2355037</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>. The specific peptide sequence of the linker is GGVRGPRV.</td> |
<td>Chrysillis Magaard Polhaus</td> | <td>Chrysillis Magaard Polhaus</td> | ||
</tr> | </tr> | ||
| Line 120: | Line 120: | ||
<td>BBa_K2355039</td> | <td>BBa_K2355039</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL.</td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of <i>Bitis gabonica</i>, <i>Bitis arietans</i> and <i>Naja nigricollis</i>. The specific peptide sequence of the linker is IDIRGPRL.</td> |
<td>Cathrine Agnete Larsen </td> | <td>Cathrine Agnete Larsen </td> | ||
</tr> | </tr> | ||
Latest revision as of 01:04, 2 November 2017
Parts
The following table is a summary of the different parts created in the project
| Part Number | Type | Description | Designer |
|---|---|---|---|
| BBa_K2355001 | Protein-domain | Peptide linker that is cleaved by Bitis arietans and Bitis gabonica venom. When translated, this linker should contain 5 x repeats of the ALK motive identified by our experiments to be cleavable by the venom of Bitis sp. | Philip Hau Soerensen |
| BBa_K2355002 | Coding | This is a modification of the amilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein | Philip Hau Soerensen |
| BBa_K2355006 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS | Chrysillis Magaard Polhaus |
| BBa_K2355008 | Protein-domain | This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. | Cathrine Agnete Larsen |
| BBa_K2355014 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is PEDDGYFV. | Chrysillis Magaard Polhaus |
| BBa_K2355015 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL. | Cathrine Agnete Larsen |
| BBa_K2355025 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is DEMDEKSE. | Chrysillis Magaard Polhaus |
| BBa_K2355026 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK | Cathrine Agnete Larsen |
| BBa_K2355032 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is RKRRAVLT. | Chrysillis Magaard Polhaus |
| BBa_K2355033 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is DDSDAATF. | Cathrine Agnete Larsen |
| BBa_K2355037 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV. | Chrysillis Magaard Polhaus |
| BBa_K2355039 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL. | Cathrine Agnete Larsen |
| BBa_K2355302 | Composite part | This part contains two major protein domains: A single chain avidin domain to bind the device to biotinylated surfaces, and amilCP with his-tag. Between the two domains, NgoMIV and AgeI restriction sites are present, to allow for insertion of RFC[25] part. | Cathrine Agnete Larsen |
| BBa_K2355313 | Composite part | This part is used for detection of snake venom. The Beta-galactosidase, encoded by lacZ, should allow for an decrease in detection time. | Philip Hau Soerensen |
