Difference between revisions of "Team:ETH Zurich/Model"

 
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    <h1 class="headline">Modeling</h1>
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<h1 class="headline">Modeling</h1>
  
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        <p>When building a bacterial system meant to function in a more complex setting than in bulk in a test tube, modeling what would its behavior be in real conditions is key to aim for a credible development. Because the experimental conditions that were available to us to develop our system (in vitro experiments) were quite far from the actual situation (in vivo tumor colonization), a significant amount of work on the modeling was needed, particularly to implement the Tumor Sensor</p>
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    <p>When building a bacterial system meant to function in a more complex setting than in bulk in a test tube, modeling what would its behavior be in real conditions is key to aim for a credible development. Because the experimental conditions that were available to us to develop our system (in vitro experiments) were quite far from the actual situation (in vivo tumor colonization), a significant amount of work on the modeling was needed, particularly to implement the Tumor Sensor</p>
  
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        <p>With our model, we could make the link between what was happening during our in vitro experiments and what would correspondingly happen in the real case scenario in the tumor.</p>
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    <p>With our model, we could make the link between what was happening during our in vitro experiments and what would correspondingly happen in the real case scenario in the tumor.</p>
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            <figure>
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    <section>
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                <!--maybe works with jpg?-->
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        <h1>Model Map</h1>
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                <img src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--overview_model.png" alt="" usemap="#Map" />
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        <p>Our project relied heavily on estimating the environment in which CATE operates, as well as the changes that it brings about after colonizing a tumor shell. The Model Map below is a rough outline of our efforts; click on it to explore our modelling in more detail!</p>
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                <map name="Map" id="Map">
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    <figure style="margin: 10px auto;">
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                    <area alt="System_requirements" title="System Specifications" href="https://2017.igem.org/Team:ETH_Zurich/Model/Environment_Sensing/system_specifications" shape="rect" coords="0,0,200,200" />
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        <img alt="Model_Overview" src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--overview_model.png" style="width: 1000px;" usemap="#Map"/>
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        <map name="Map" id="Map">
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                </map>
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            <area alt="" title="System Specifications" href="/Team:ETH_Zurich/Model/Environment_Sensing/system_specifications" shape="poly" coords="216,23,397,23,412,31,417,43,418,87,413,99,400,107,217,107,204,99,199,88,198,44,204,30">
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            <area alt="" title="Functional Parameter Space" href="/Team:ETH_Zurich/Model/Environment_Sensing/parameter_space" shape="poly" coords="478,89,655,90,672,98,677,107,679,150,672,164,661,172,477,173,465,166,459,160,457,111,463,96">
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            <area alt="" title="Parts Design Guidelines" href="/Team:ETH_Zurich/Model/Environment_Sensing/parameter_space#GuidelineToParts" shape="poly" coords="777,111,959,111,974,118,980,128,982,237,976,250,965,258,781,259,765,252,758,240,757,136,764,122">
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            <area alt="" title="Experiments" href="/Team:ETH_Zurich/Experiments" shape="poly" coords="783,381,953,380,955,453,785,453">
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            <area alt="" title="Parameter Fitting" href="/Team:ETH_Zurich/Model/Environment_Sensing/parameter_fitting" shape="poly" coords="505,309,687,309,701,315,705,324,707,375,697,385,687,390,506,392,493,384,487,375,485,328,493,314">
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            <area alt="" title="Our own Hybrid Promoter" href="/Team:ETH_Zurich/Model/Environment_Sensing/AND_gate_fitting" shape="poly" coords="509,417,685,417,699,423,705,434,706,495,698,508,685,512,507,513,493,507,485,497,487,431,495,421">
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            <area alt="" title="Tumor Sensor Model" href="/Team:ETH_Zurich/Model/Environment_Sensing/model" shape="poly" coords="247,211,428,211,440,219,447,228,448,278,441,287,429,294,249,297,233,288,227,279,227,233,234,219">
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            <area alt="" title="Voila" href="/Team:ETH_Zurich/Model/In_Silico_Final" shape="poly" coords="86,363,344,362,357,369,365,381,366,519,359,528,345,537,84,536,74,527,67,517,67,385,75,369">
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        </map>
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    </figure>
  
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            </figure>
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</section>
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        <p>As a result, we have been able to give the wet lab initial and follow-up design guidelines to get our bacteria better at <a href="https://2017.igem.org/Team:ETH_Zurich/Model/Environment_Sensing">targeting tumor specifically</a>, and exhibit a <a href="https://2017.igem.org/Team:ETH_Zurich/Model/Thermal_Triggering">reasonable response time for triggering</a> the release of azurin.</p>
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Latest revision as of 01:13, 2 November 2017

Modeling

When building a bacterial system meant to function in a more complex setting than in bulk in a test tube, modeling what would its behavior be in real conditions is key to aim for a credible development. Because the experimental conditions that were available to us to develop our system (in vitro experiments) were quite far from the actual situation (in vivo tumor colonization), a significant amount of work on the modeling was needed, particularly to implement the Tumor Sensor

With our model, we could make the link between what was happening during our in vitro experiments and what would correspondingly happen in the real case scenario in the tumor.

Model Map

Our project relied heavily on estimating the environment in which CATE operates, as well as the changes that it brings about after colonizing a tumor shell. The Model Map below is a rough outline of our efforts; click on it to explore our modelling in more detail!

Model_Overview