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| | {{UFlorida/Footer}} | | {{UFlorida/Footer}} |
| | <html> | | <html> |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css"
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| − | <link href="https://fonts.googleapis.com/css?family=Montserrat" rel="stylesheet" type="text/css">
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| − | <script
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| − | src="https://code.jquery.com/jquery-3.2.1.min.js"
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| − | integrity="sha256-hwg4gsxgFZhOsEEamdOYGBf13FyQuiTwlAQgxVSNgt4="
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| − | crossorigin="anonymous"></script>
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| − | <style>
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| − | @charset "UTF-8";
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| − | @import url(font-awesome.min.css);
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| − | @import "https://fonts.googleapis.com/css?family=Raleway:100,300,600";
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| − |
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| − | /* Banner */
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| − |
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| − | #banner {
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| − | padding: 22em 0 12em 0;
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| − | background-image: url('https://static.igem.org/mediawiki/2017/5/59/T--UFlorida--InterLab_Header.png');
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| − | background-size: cover;
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| − | background-position: top;
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| − | background-attachment: fixed;
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| − | background-repeat: no-repeat;
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| − | text-align: center;
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| − | }
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| − | #banner h1 {
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| − | font-size: 3.5em;
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| − | font-weight: 100;
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| − | color: #fff;
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| − | line-height: 1em;
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| − | margin: 0 0 0.5em 0;
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| − | padding: 0;
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| − | }
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| − | #banner p {
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| − | font-size: 1em;
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| − | color: rgba(255, 255, 255, 0.55);
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| − | margin-bottom: 1.75em;
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| − | }
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| − | @media only screen and (min-device-width: 768px) and (max-device-width: 1024px) and (orientation: landscape) {
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| − | #banner {
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| − | background-attachment: scroll;
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| − | }
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| − | }
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| − | @media screen and (max-width: 1280px) {
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| − | #banner {
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| − | padding: 10em 2em 8em 2em;
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| − | }
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| − | }
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| − | @media screen and (max-width: 980px) {
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| − | #banner {
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| − | background-attachment: scroll;
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| − | }
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| − | }
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| − | @media screen and (max-width: 736px) {
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| − | #banner {
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| − | padding: 8em 1.5em 6em 1.5em;
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| − | }
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| − | #banner h1 {
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| − | font-size: 2.5em;
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| − | }
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| − | #banner p {
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| − | font-size: .9em;
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| − | }
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| − | }
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| − | @media screen and (max-width: 480px) {
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| − | #banner {
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| − | padding: 6em 1.5em 4em 1.5em;
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| − | }
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| − | }
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| − | </style>
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| − | <script>
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| − | (function($) {
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| − |
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| − | // Breakpoints.
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| − | skel.breakpoints({
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| − | xlarge: '(max-width: 1680px)',
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| − | large: '(max-width: 1280px)',
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| − | medium: '(max-width: 980px)',
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| − | small: '(max-width: 736px)',
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| − | xsmall: '(max-width: 480px)'
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| − | });
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| − | $(function() {
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| − | var $window = $(window),
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| − | $body = $('body');
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| − | // Disable animations/transitions until the page has loaded.
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| − | $body.addClass('is-loading');
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| − | $window.on('load', function() {
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| − | window.setTimeout(function() {
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| − | $body.removeClass('is-loading');
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| − | }, 100);
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| − | });
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| − | // Prioritize "important" elements on medium.
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| − | skel.on('+medium -medium', function() {
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| − | $.prioritize(
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| − | '.important\\28 medium\\29',
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| − | skel.breakpoint('medium').active
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| − | );
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| − | });
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| − | // Off-Canvas Navigation.
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| − | // Navigation Panel.
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| − | $(
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| − | '<div id="navPanel">' +
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| − | $('#nav').html() +
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| − | '<a href="#navPanel" class="close"></a>' +
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| − | '</div>'
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| − | )
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| − | .appendTo($body)
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| − | .panel({
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| − | delay: 500,
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| − | hideOnClick: true,
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| − | hideOnSwipe: true,
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| − | resetScroll: true,
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| − | resetForms: true,
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| − | side: 'left'
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| − | });
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| − |
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| − | // Fix: Remove transitions on WP<10 (poor/buggy performance).
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| − | if (skel.vars.os == 'wp' && skel.vars.osVersion < 10)
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| − | $('#navPanel')
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| − | .css('transition', 'none');
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| − |
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| − | });
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| − |
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| − | })(jQuery);
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| − | </script>
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| − | </head>
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| − | <body>
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| − |
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| − | <!-- Banner -->
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| − | <section id="banner">
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| − | <h1></h1>
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| − | <p></p>
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| − | </section>
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| | <h3>Overview</h3> | | <h3>Overview</h3> |
| − | <p>Directly comparing measurements is a challenge for many synthetic biology researchers. Fluorescent measurement is difficult for researchers to compare because it can be analyzed and reported in a variety of ways. Through the InterLab 2017 study, the iGEM Measurement Committee seeks to provide researchers with a standard for measuring expression of GFP in a plate reader.</p>
| + | Directly comparing measurements is a challenge for many synthetic biology researchers. Fluorescent measurement is difficult for researchers to compare because it can be analyzed and reported in a variety of ways. Through the InterLab 2017 study, the iGEM Measurement Committee seeks to provide researchers with a standard for measuring expression of GFP in a plate reader. |
| − | | + | <br><br> |
| − | <p>iGEM Teams worldwide contributed to the InterLab study by transforming DH5a E. coli with six test devices (BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, and BBa_J364005), as well as a positive (BBa_I20270), and negative control (BBa_R0040). Teams then measured the fluorescence of the bacteria in a plate reader over a 6-hour period. </p> | + | iGEM Teams worldwide contributed to the InterLab study by transforming DH5a E. coli with six test devices (BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, and BBa_J364005), as well as a positive (BBa_I20270), and negative control (BBa_R0040). Teams then measured the fluorescence of the bacteria in a plate reader over a 6-hour period. |
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| | <h3>Materials and Methods</h3> | | <h3>Materials and Methods</h3> |
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| | <li>Two colonies were selected from each of plate and inoculated it in 5 mL LB medium + Chloramphenicol in separate 50 ml Falcon Tubes. The Falcon tubes were then covered tightly with foil. The cells were grown overnight (16 hours) at 37°C and 220 rpm.</li> | | <li>Two colonies were selected from each of plate and inoculated it in 5 mL LB medium + Chloramphenicol in separate 50 ml Falcon Tubes. The Falcon tubes were then covered tightly with foil. The cells were grown overnight (16 hours) at 37°C and 220 rpm.</li> |
| | </ol> | | </ol> |
| − | <div class="row-sm-2"> | + | <div class="row"> |
| | <div class="col-sm-2"> | | <div class="col-sm-2"> |
| − | <img src="https://static.igem.org/mediawiki/2017/7/77/T--UFlorida--InterLab_Plate_B.jpeg" height="200"> | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/7/77/T--UFlorida--InterLab_Plate_B.jpeg" height="200"> |
| | </div> | | </div> |
| | <div class="col-sm-2"> | | <div class="col-sm-2"> |
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| | </div> | | </div> |
| | </div> | | </div> |
| − | | + | <br> |
| − | <div class="row-sm-2"> | + | <div class="row"> |
| | <div class="col-sm-2"> | | <div class="col-sm-2"> |
| | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--UFlorida--InterLab_Plate_J.jpeg" height="200"> | | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--UFlorida--InterLab_Plate_J.jpeg" height="200"> |
