Difference between revisions of "Team:SDU CHINA/basic parts"

 
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<div class="all">
 
<div class="all">
  
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<div class="title"><span class="STYLE11"><strong>Table of the Basic Parts</strong> we submitted to the BioBrick registry</span> </div>
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<div class="title"><span class="STYLE13"><strong>Table of the Basic Parts</strong> <br><br>we submitted to the BioBrick registry</span> </div>
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        <td valign="top" ><p class="STYLE7" >Part Number </p></td>
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      <th scope="col"><p >Part Number </p></th>
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      <td valign="top" ><p class="STYLE7" >Name </p></td>
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      <th scope="col"><p >Name </p></th>
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       <td valign="top" ><p class="STYLE7" >Type </p></td>
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       <th scope="col"><p >Type </p></th>
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       <td valign="top" ><p class="STYLE7" >Length </p></td>
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       <th scope="col"><p >Length </p></th>
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      <td valign="top" ><p class="STYLE7" >Description </p></td>
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      <th scope="col"><p >Description </p></th>
 
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         <td valign="top" ><p class="STYLE8" >BBa_K2321000 </p></td>
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         <td valign="top" ><p class="STYLE8" ><a href='http://parts.igem.org/Part:BBa_K2321000'>BBa_K2321000</a></p></td>
 
       <td valign="top" ><p class="STYLE8" > hTERT promoter </p></td>
 
       <td valign="top" ><p class="STYLE8" > hTERT promoter </p></td>
 
       <td valign="top" ><p class="STYLE8" >Regulatory </p></td>
 
       <td valign="top" ><p class="STYLE8" >Regulatory </p></td>
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         <td valign="top" ><p class="STYLE8" >BBa_K2321001 </p></td>
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         <td valign="top" ><p class="STYLE8" ><a href='http://parts.igem.org/Part:BBa_K2321001'>BBa_K2321001</a></p></td>
 
       <td valign="top" ><p class="STYLE8" >Survivin promoter </p></td>
 
       <td valign="top" ><p class="STYLE8" >Survivin promoter </p></td>
 
       <td valign="top" ><p class="STYLE8" >Regulatory </p></td>
 
       <td valign="top" ><p class="STYLE8" >Regulatory </p></td>
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         <td valign="top" ><p class="STYLE8" >BBa_K2321002 </p></td>
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         <td valign="top" ><p class="STYLE8" ><a href='http://parts.igem.org/Part:BBa_K2321002'>BBa_K2321002</a></p></td>
 
       <td valign="top" ><p class="STYLE8" >Luciferase </p></td>
 
       <td valign="top" ><p class="STYLE8" >Luciferase </p></td>
 
       <td valign="top" ><p class="STYLE8" >Reporter </p></td>
 
       <td valign="top" ><p class="STYLE8" >Reporter </p></td>
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<div class="content">Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important.
 
<div class="content">Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important.
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In order to explore the specificity and sensitivity of starting efficiency for each promoter, they were respectively inserted into double luciferase reporter vector. Then we selected two kinds of NSCLC cell lines, including A549， and measured the relative luciferase expression activity of the plasmids in different cell lines and different time points.</div>
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In order to explore the specificity and sensitivity of starting efficiency for each promoter, they were respectively inserted into luciferase reporter vector. Then we selected NSCLC cell lines A549， and measured the relative luciferase expression activity of the consructed plasmids in A549 cell line in different time points,which represent the starting efficiency of each promoter.</div>
  
 
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<p><strong>Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></p>
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<br>
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<p><strong>TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.</strong></p>
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<div class="img"><strong>Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></div>
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<div class="img"><strong>TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.</strong></div>
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<p><strong>Figure 1: The express efficiency of hTERT promoter in A549 cell line.</strong></p>
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<div class="img"><strong>Figure 1: The express efficiency of hTERT promoter in A549 cell line.</strong></div>
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  <p><img src="https://static.igem.org/mediawiki/2017/4/45/Basic3.png" style="width:60%">     </p>
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<img src="https://static.igem.org/mediawiki/2017/4/45/Basic3.png" style="width:80%">   </div>
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  <p><strong>Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong> </p>
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  <p><strong>TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.</strong>
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<div class="img"><strong>Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on </strong></div>
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      </p>
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<div class="img"><strong>TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.</strong></div>
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<p><span class="STYLE8"><strong>Figure 2 :The express efficiency of survivin promoter in A549 cell line</strong></span>.</p>
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<div class="img"><strong>Figure 2 :The express efficiency of survivin promoter in A549 cell line</strong></span>.</div>
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<h3 align="center" class="STYLE15">For more details, please refer to the <a href="https://2017.igem.org/Team:SDU_CHINA/Results">results</a>.</h3>
 
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<h3 align="center" class="STYLE8"><strong>For more details, please refer to the <a href="https://2017.igem.org/Team:SDU_CHINA/Measurement">results</a>.</strong></h3>
 
  
 
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Latest revision as of 01:26, 2 November 2017








Table of the Basic Parts

we submitted to the BioBrick registry




Part Number

Name

Type

Length

Description

BBa_K2321000

 hTERT promoter

Regulatory

809

tumor-specific promoter

BBa_K2321001

Survivin promoter

Regulatory

1024

tumor-specific promoter

BBa_K2321002

Luciferase

Reporter

1653

firefly luciferase reporter


Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important. In order to explore the specificity and sensitivity of starting efficiency for each promoter, they were respectively inserted into luciferase reporter vector. Then we selected NSCLC cell lines A549， and measured the relative luciferase expression activity of the consructed plasmids in A549 cell line in different time points,which represent the starting efficiency of each promoter.




Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on
TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.




Figure 1: The express efficiency of hTERT promoter in A549 cell line.




Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on
TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.




Figure 2 :The express efficiency of survivin promoter in A549 cell line.

For more details, please refer to the results.