Difference between revisions of "Team:Jilin China/Improve"

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<div class="banner"><div class="menu">Improvement</div><img src="https://static.igem.org/mediawiki/2017/b/b1/T--Jilin_China--_sec_bg_t.jpg"></div>
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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    <div class="sec_box">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <div class="h1_title">1.Improvement in detecting method in DmpR response intensity.</div>
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<div class="h1_content">
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<div class="clear"></div>
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<p>(1) We realized the quantitative measurement in DmpR response intensity using Renilla Luciferase. Compared to GFP <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/DmpR">team Peking-2013</a> used, we can detect DmpR response intensity more accurately.
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<div class="pic_box center">
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    <img src="https://static.igem.org/mediawiki/2017/4/4f/T--Jilin_China--improvement01.png"width="500"  /><br />
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<h1>Improve</h1>
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    <img src="https://static.igem.org/mediawiki/2017/1/1d/T--Jilin_China--improvement02.png"width="500"  /><br />
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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    Figure 1. DmpR circuit. (A) Peking-2013 (B) Jilin-2017
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    </div>
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<h3>Gold Medal Criterion #2</h3>
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</p>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<p>(2) Renilla Luciferase enabled us to conduct the measurement <i>in vivo</i>, which provided an easier and faster method in detecting.</p>
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</div>
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<br><br>
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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<div class="h1_title">2.Improvement in response intensity toward certain phenolics.</div>
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<div class="h1_content">
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<p>Team Jilin_China in 2017 did several mutagenesis in sensor domain of DmpR. To compare the difference between <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/DmpR">Peking-2013 DmpR</a> and our mutated DmpR downstreaming of the same constitutive promoter, we chose phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol as the inducer. Expression level of Renilla Luciferase downstream of <i>dmp</i> operon (P<sub>0</sub> promoter) was used to indicate the response intensity towards different phenolics. Comparisons were based on ratio of different DmpR response and mock's toward phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol respectively.
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</div>
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<div class="pic_box center">
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    <img src="https://static.igem.org/mediawiki/2017/b/be/T--Jilin_China--improvement03.png"width="500"  /><br />
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    Figure 2. The device we constructed to do comparison between peking-2013 dmpR and our mutated DmpR
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    </div>
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</p><br />
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<p>
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It turned out that both DmpR can response to phenol, 2-CP, pyrocatechol. However, compared to Peking-DmpR, our DmR showed better response to phenol and pyrocatechol.
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<div class="pic_box center">
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    <img src="https://static.igem.org/mediawiki/2017/7/7c/T--Jilin_China--improvement04.png" width="60%" /><br />                              
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    Figure 3. DmpR response sensitivity toward different phenolics. Jilin-2017-DmpR showed better response to phenol and pyrocatechol.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;★，p<0.05
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{{Jilin_footer}}

Latest revision as of 01:27, 2 November 2017

1.Improvement in detecting method in DmpR response intensity.

(1) We realized the quantitative measurement in DmpR response intensity using Renilla Luciferase. Compared to GFP team Peking-2013 used, we can detect DmpR response intensity more accurately.



Figure 1. DmpR circuit. (A) Peking-2013 (B) Jilin-2017

(2) Renilla Luciferase enabled us to conduct the measurement in vivo, which provided an easier and faster method in detecting.

2.Improvement in response intensity toward certain phenolics.

Team Jilin_China in 2017 did several mutagenesis in sensor domain of DmpR. To compare the difference between Peking-2013 DmpR and our mutated DmpR downstreaming of the same constitutive promoter, we chose phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol as the inducer. Expression level of Renilla Luciferase downstream of dmp operon (P0 promoter) was used to indicate the response intensity towards different phenolics. Comparisons were based on ratio of different DmpR response and mock's toward phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol respectively.


Figure 2. The device we constructed to do comparison between peking-2013 dmpR and our mutated DmpR


It turned out that both DmpR can response to phenol, 2-CP, pyrocatechol. However, compared to Peking-DmpR, our DmR showed better response to phenol and pyrocatechol.


Figure 3. DmpR response sensitivity toward different phenolics. Jilin-2017-DmpR showed better response to phenol and pyrocatechol.       ★，p<0.05