Difference between revisions of "Team:UFlorida/Results"

 
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<h1>Results</h1>
 
<h1>Results</h1>
  
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<p>Here you can describe the results of your project and your future plans. </p>
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Most of our time on the project was spent trying to get all three genes into one cell. We succeeded in transforming cells with three plasmids, theoretically containing ARO9, ARO 10 and ADH1, but were unable to confirm the presence of ARO9.
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<br><br>
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We transformed plasmids containing ARO10 in pETDUET-1 and ADH1 in pCOLADUET-1 in BL21 cells, but were unable to confirm the presence of ARO9 in a DUET vector either through gel electrophoresis or through Sanger Sequencing. ARO9 encodes aromatic animo acid transferase II, which is the first enzyme in the tryptophol pathway. ARO10 in pETDUET-1 and ADH in pCOLADUET-1 were validated using Sanger Sequencing by Genewiz®
  
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<h5>What should this page contain?</h5>
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<br><br>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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</ul>
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<h5>You should also describe what your results mean: </h5>
 
  
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We ran an HPLC Standard of tryptophol in King's Medium B. Here, tryptophol shows an elution peak at 162 m/z.
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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</ul>
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</div>
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<center>
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            <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/0/0b/T--UFlorida--HPLC_Standard.png">
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</center>
  
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<div class="clear"></div>
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We are planning to conduct HPLC using a filtered bacterial culture, and compare the graph generated to the graph of our tryptophol standard. The HPLC results should be available for our poster and presentation. Currently, we are conducting Kirby Bower tests on a fungus in the Chytridiomycota family using tryptophol for one plate, water for one plate, and liquid cultures of our bacteria for another plate. We expect to have the results from these tests for our poster and presentation.
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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</ul>
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</div>
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<div class="column half_size" >
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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</ul>
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</div>
 
</div>

Latest revision as of 01:32, 2 November 2017

Results

Most of our time on the project was spent trying to get all three genes into one cell. We succeeded in transforming cells with three plasmids, theoretically containing ARO9, ARO 10 and ADH1, but were unable to confirm the presence of ARO9.

We transformed plasmids containing ARO10 in pETDUET-1 and ADH1 in pCOLADUET-1 in BL21 cells, but were unable to confirm the presence of ARO9 in a DUET vector either through gel electrophoresis or through Sanger Sequencing. ARO9 encodes aromatic animo acid transferase II, which is the first enzyme in the tryptophol pathway. ARO10 in pETDUET-1 and ADH in pCOLADUET-1 were validated using Sanger Sequencing by Genewiz®

We ran an HPLC Standard of tryptophol in King's Medium B. Here, tryptophol shows an elution peak at 162 m/z.
We are planning to conduct HPLC using a filtered bacterial culture, and compare the graph generated to the graph of our tryptophol standard. The HPLC results should be available for our poster and presentation. Currently, we are conducting Kirby Bower tests on a fungus in the Chytridiomycota family using tryptophol for one plate, water for one plate, and liquid cultures of our bacteria for another plate. We expect to have the results from these tests for our poster and presentation.