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− | <div class="slide slide-image" style="background-image: url('https://static.igem.org/mediawiki/2017/f/ | + | <div class="slide slide-image" style="background-image: url('https://static.igem.org/mediawiki/2017/f/fa/Arttt.png')"></div> |
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<h1>Academics</h1> | <h1>Academics</h1> | ||
− | <p> | + | <p>Teams that develop fundamental projects are faced with certain challenges: mainly these projects require a lot of specific theoretical knowledge and they are typically characterized by low immediate applicability. Therefore, first we had to rely on opinions and suggestions of other scientists.</p> |
<div class="readmore" data-modal="1">read more</div> | <div class="readmore" data-modal="1">read more</div> | ||
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<h1>Survey</h1> | <h1>Survey</h1> | ||
− | <p>We completed the investigation of the field | + | <p>We completed the investigation of the field by surveying the potential end-users of our SynORI system by reaching out to the hands-on laboratories around the globe.</p> |
<div class="readmore" data-modal="3">read more</div> | <div class="readmore" data-modal="3">read more</div> | ||
</div> | </div> | ||
<div class="slide-right-content slide-right-content-4 hidden"> | <div class="slide-right-content slide-right-content-4 hidden"> | ||
<h1>Bioethics</h1> | <h1>Bioethics</h1> | ||
− | <p> | + | <p>Fundamental projects require specific knowledge and are extremely tough to demonstrate to the society, not to mention the difficulty of collecting feedback from the lay public. With regard to that, we launched a bioart project "SyNbiosis", which aimed to gather scientists, lay public and artists to discuss and reflect on ethical, biosafety, social, and legal aspects of our project.</p> |
<div class="readmore" data-modal="4">read more</div> | <div class="readmore" data-modal="4">read more</div> | ||
</div> | </div> | ||
<div class="slide-right-content slide-right-content-5 hidden"> | <div class="slide-right-content slide-right-content-5 hidden"> | ||
<h1>Bioart Exhibition</h1> | <h1>Bioart Exhibition</h1> | ||
− | <p> | + | <p>We thought of many ideas to involve the public and integrate their concerns into our project but finally decided that an interactive bioart exhibition is something that has never been done before in the iGEM competition. In addition, we prepared a short guide for the creation of bioart exhibitions. </p> |
<div class="readmore" data-modal="5">read more</div> | <div class="readmore" data-modal="5">read more</div> | ||
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<ul class="navigation"> | <ul class="navigation"> | ||
− | + | <li><a href="/Team:Vilnius-Lithuania/HP/Silver">Silver</a></li> | |
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<li> | <li> | ||
− | <a href=" | + | <a href="/Team:Vilnius-Lithuania/HP/Gold_Integrated" class="active">Integrated</a> |
<ul> | <ul> | ||
<li class="is-current"><div class="sli-item sli-item-1">Academics</div></li> | <li class="is-current"><div class="sli-item sli-item-1">Academics</div></li> | ||
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− | <li><a href=" | + | <li><a href="/Team:Vilnius-Lithuania/Engagement">Education & Public Engagement</a></li> |
− | <li><a href=" | + | <li><a href="/Team:Vilnius-Lithuania/Collaborations">Collaborations</a></li> |
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<li><p>scientists from the laboratory of protein-nucleic acid interactions gave crucial advice on managing and optimizing our workflow, which helped us to plan our project timeline. It allowed us to adjust and model our work with more flexibility which also became reflected in the flexibility of the SynORI system itself.</p></li> | <li><p>scientists from the laboratory of protein-nucleic acid interactions gave crucial advice on managing and optimizing our workflow, which helped us to plan our project timeline. It allowed us to adjust and model our work with more flexibility which also became reflected in the flexibility of the SynORI system itself.</p></li> | ||
</ul> | </ul> | ||
− | + | <div class="img-cont"> | |
− | + | ||
<img src="https://static.igem.org/mediawiki/2017/b/ba/T--Vilnius-Lithuania--coinsmeet.jpeg" alt="img"> | <img src="https://static.igem.org/mediawiki/2017/b/ba/T--Vilnius-Lithuania--coinsmeet.jpeg" alt="img"> | ||
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</div> | </div> | ||
+ | <p>After receiving valuable insights from scientists at our home university, we turned to the biotech industry, realizing that a critical aspect of modern synthetic biology project is its applicability and potential influence to the overall development of biotechnology.</p> | ||
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<p>Although the experts in the sector of biotechnology provided us very useful information, we wanted to complete the investigation of how exactly we should build our SynORI project by interacting with the hands-on laboratories around the globe.</p> | <p>Although the experts in the sector of biotechnology provided us very useful information, we wanted to complete the investigation of how exactly we should build our SynORI project by interacting with the hands-on laboratories around the globe.</p> | ||
<div class="img-cont"> | <div class="img-cont"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/f/fd/T--Vilnius-Lithuania--industry2.jpeg" alt="img"> |
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<h1>Survey</h1> | <h1>Survey</h1> | ||
− | <h5>We completed the investigation of | + | <h5>We completed the investigation of our field of exploration by surveying the potential end-users of our SynORI system. To do this we reached out to various hands-on laboratories around the globe.</h5> |
<p>We conducted a survey which aimed to investigate the issues of cotransformation and plasmid incompatibility and to gather the information necessary to assure that SynORI system would be beneficial and widely used in the laboratories around the globe. The survey consisted of 15 questions and involved local experts as well as international laboratories, which we reached with the help of many teams from the iGEM competition. The results of the survey are summed up in following points:</p> | <p>We conducted a survey which aimed to investigate the issues of cotransformation and plasmid incompatibility and to gather the information necessary to assure that SynORI system would be beneficial and widely used in the laboratories around the globe. The survey consisted of 15 questions and involved local experts as well as international laboratories, which we reached with the help of many teams from the iGEM competition. The results of the survey are summed up in following points:</p> | ||
− | < | + | <ul> |
− | <li><p> | + | <li><p>Respondents named plasmid incompatibility to be the major disadvantage of cotransformation process.</p></li> |
− | <li><p> | + | <br> |
− | <li><p> | + | <div class="img-cont"> |
+ | <img src="https://static.igem.org/mediawiki/parts/c/c8/T--Vilnius-Lithuania--grafikai7.png" alt="img"> | ||
+ | <ul/> | ||
+ | </div> | ||
+ | <br> | ||
+ | <li><p>The majority of respondents agreed that they would transform bacteria with more plasmids than usual if a system enabling plasmid compatibility existed.</p></li> | ||
+ | <br> | ||
+ | <div class="img-cont"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/4/42/T--Vilnius-Lithuania--grafikai2.png" alt="img"> | ||
+ | <ul/> | ||
+ | </div> | ||
+ | <br> | ||
+ | <li><p>75.9% of respondents confirmed that an ability to keep a fixed ratio of different types of plasmids intracellularly would be useful in their experiments. It provided us a critically important feedback on one of our main project goals.</p></li> | ||
+ | <br> | ||
+ | <div class="img-cont"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/f/ff/T--Vilnius-Lithuania--grafikai6.png" alt="img"> | ||
+ | <ul/> | ||
+ | </div> | ||
+ | <br> | ||
<p>As we planned to make a system for multi-plasmid cotransformation, we focused some survey questions on biosafety. We wondered, whether different laboratories tried to avoid the usage of multiple antibiotics.</p> | <p>As we planned to make a system for multi-plasmid cotransformation, we focused some survey questions on biosafety. We wondered, whether different laboratories tried to avoid the usage of multiple antibiotics.</p> | ||
− | <li><p>More than 95% of surveyed scientists | + | <br> |
+ | <ul> | ||
+ | <li><p>More than 95% of surveyed scientists use antibiotics to select transformants. They mostly used a combination of two antibiotics, however 6.9% of respondents used more than 3 antibiotics for selection on regular basis. It corresponded to the number of different plasmids in their experiments.</p></li> | ||
+ | <br> | ||
+ | <div class="img-cont"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/e/ec/T--Vilnius-Lithuania--grafikai4.png" alt="img"> | ||
+ | </div> | ||
+ | <br> | ||
<li><p>Most of respondents did not try to find an alternative to antibiotics by using another selection system.</p></li> | <li><p>Most of respondents did not try to find an alternative to antibiotics by using another selection system.</p></li> | ||
+ | <br> | ||
+ | <div class="img-cont"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/e/ec/T--Vilnius-Lithuania--grafikai4.png" alt="img"> | ||
+ | </div> | ||
+ | <br> | ||
<li><p>All respondents agreed that they would use another cotransformants' selection system, that could decrease the usage of antibiotics. This has prompted our focus on creating an improved selection method for multi-plasmid systems.</p></li> | <li><p>All respondents agreed that they would use another cotransformants' selection system, that could decrease the usage of antibiotics. This has prompted our focus on creating an improved selection method for multi-plasmid systems.</p></li> | ||
− | < | + | <br> |
− | </ | + | <div class="img-cont"> |
+ | <img src="https://static.igem.org/mediawiki/parts/7/7c/T--Vilnius-Lithuania--grafikai1.png" alt="img"> | ||
+ | </div> | ||
+ | <br> | ||
+ | </ul> | ||
<p>It is therefore obvious, that our SynORI project benefited much from involving the local scientists on the scale of technical and environmental issues, as well as it was both approved and improved by three dimensions of biotechnology experts: academic staff, biotech industry and both local and international hands-on laboratories.</p> | <p>It is therefore obvious, that our SynORI project benefited much from involving the local scientists on the scale of technical and environmental issues, as well as it was both approved and improved by three dimensions of biotechnology experts: academic staff, biotech industry and both local and international hands-on laboratories.</p> | ||
+ | <br> | ||
<p>Foundational teams need to consider wider implications of their project as well. Fundamental projects require specific knowledge and are extremely tough to demonstrate to the society, not to mention the difficulty of collecting feedback from the lay public. With regard to that, we launched a bioart project "SyNbiosis", which aimed to gather scientists, lay public and artists to discuss and reflect on ethical, biosafety, social, and legal aspects of our project.</p> | <p>Foundational teams need to consider wider implications of their project as well. Fundamental projects require specific knowledge and are extremely tough to demonstrate to the society, not to mention the difficulty of collecting feedback from the lay public. With regard to that, we launched a bioart project "SyNbiosis", which aimed to gather scientists, lay public and artists to discuss and reflect on ethical, biosafety, social, and legal aspects of our project.</p> | ||
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<h1>Bioethics</h1> | <h1>Bioethics</h1> | ||
+ | <h5>Fundamental projects require specific knowledge and are extremely tough to demonstrate to the society, not to mention the difficulty of collecting feedback from the lay public. With regard to that, we launched a bioart project "SyNbiosis", which aimed to gather scientists, lay public and artists to discuss and reflect on ethical, biosafety, social, and legal aspects of our project.</h5> | ||
<p>Synthetic biology is one of the most debatable fields of science which elicits both public's flair for incredible inventions and great fears of the unknown. Today, the advancement of this field of science enabled the synthesis of customized genetic elements and systems for various applications on an unprecedented scale. Contemporaneously, this raised a wave of critical reconsideration of the role and meaning of human creation itself. Synthetic biologists often refer to a famous quote by theoretical physicist Richard Feynman: "What I cannot create, I do not understand", but do we really understand what we create? In addition, synthetic biology raises many more similar questions. Are we satisfied with current definitions of life? Who is in charge to define the future of humanity? What are the boundaries between human and non-human agencies?</p> | <p>Synthetic biology is one of the most debatable fields of science which elicits both public's flair for incredible inventions and great fears of the unknown. Today, the advancement of this field of science enabled the synthesis of customized genetic elements and systems for various applications on an unprecedented scale. Contemporaneously, this raised a wave of critical reconsideration of the role and meaning of human creation itself. Synthetic biologists often refer to a famous quote by theoretical physicist Richard Feynman: "What I cannot create, I do not understand", but do we really understand what we create? In addition, synthetic biology raises many more similar questions. Are we satisfied with current definitions of life? Who is in charge to define the future of humanity? What are the boundaries between human and non-human agencies?</p> | ||
+ | <div class="img-cont"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/ee/T--Vilnius-Lithuania--biosafety2.jpeg | ||
+ | " alt="img"> | ||
+ | </div> | ||
<p>Set along with the challenges of contemporary life, scientists are in a constant need to compare and contrast their definitions of creation, innovation and engineering of life with the public, specialists in the humanities, social sciences and arts. Therefore, we set a discussion which created an opportunity for collective thinking on the progression of synthetic biology and life sciences within the perspective of big themes of ethical responsibility, safety and future of humanity. We presented ethical issues and biosafety concerns relevant to the field of synthetic biology, the construction of synthetic life, and our project, as well as explained the importance of establishing a mutual partnership between scientists, lay public, and specialists of social sciences and arts. In addition to the lecture and discussion, we documented the experience and shift of minds of the participants by asking to express how they felt about our SynORI project before and after the event. We have learnt that on ethical and social grounds people mostly feared the reduction of the value of life itself. Almost equally important were the legal and environmental concerns of the domestication of nature. We have realized that all these issues apply to our project therefore extended the static investigation of ethical aspects of synthetic biology as we took it to the next level by setting up an interactive exhibition to embrace the mutual partnership with the society.</p> | <p>Set along with the challenges of contemporary life, scientists are in a constant need to compare and contrast their definitions of creation, innovation and engineering of life with the public, specialists in the humanities, social sciences and arts. Therefore, we set a discussion which created an opportunity for collective thinking on the progression of synthetic biology and life sciences within the perspective of big themes of ethical responsibility, safety and future of humanity. We presented ethical issues and biosafety concerns relevant to the field of synthetic biology, the construction of synthetic life, and our project, as well as explained the importance of establishing a mutual partnership between scientists, lay public, and specialists of social sciences and arts. In addition to the lecture and discussion, we documented the experience and shift of minds of the participants by asking to express how they felt about our SynORI project before and after the event. We have learnt that on ethical and social grounds people mostly feared the reduction of the value of life itself. Almost equally important were the legal and environmental concerns of the domestication of nature. We have realized that all these issues apply to our project therefore extended the static investigation of ethical aspects of synthetic biology as we took it to the next level by setting up an interactive exhibition to embrace the mutual partnership with the society.</p> | ||
<div class="img-cont"> | <div class="img-cont"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/1/ | + | <img src="https://static.igem.org/mediawiki/2017/1/1d/Bioeth.png" alt="img"> |
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<div class="modal-close"></div> | <div class="modal-close"></div> | ||
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− | <h1>Bioart | + | <h1>Bioart Exhibition</h1> |
− | < | + | <p>We thought of many ideas to involve the public and integrate their concerns into our project but finally decided that an interactive bioart exhibition is something that has never been done before in the iGEM competition. Art is an universal mean of communication by which our team could simply explain the project to the public. People felt comfortable enough to participate as our artistic activities required no strict biological education. Participants expressed their feelings and ideas, which we integrated into the development of our project.</p> |
<div class="img-cont"> | <div class="img-cont"> | ||
<img src="https://static.igem.org/mediawiki/2017/b/b6/T--Vilnius-Lithuania--paroda6.jpeg" alt="img"> | <img src="https://static.igem.org/mediawiki/2017/b/b6/T--Vilnius-Lithuania--paroda6.jpeg" alt="img"> | ||
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</div> | </div> | ||
<p>Our exhibition consisted of three parts:</p> | <p>Our exhibition consisted of three parts:</p> | ||
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<div class="img-cont"> | <div class="img-cont"> | ||
<img src="https://static.igem.org/mediawiki/2017/f/f3/T--Vilnius-Lithuania--paroda7.jpeg" alt="img"> | <img src="https://static.igem.org/mediawiki/2017/f/f3/T--Vilnius-Lithuania--paroda7.jpeg" alt="img"> | ||
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</div> | </div> | ||
<p>iGEM projects are not simply scientific works, but are also keen to involve and be relevant to the society. Our ethics and bioart exhibition activities provided insights, how fundamental projects, that deal with improving methods of construction of GMMs, should be presented and what concerns they elicit. Our human practices made us more aware and socially sensitive, and we integrated this approach to our project making it more public-friendly and open for discussion to debunk the fears and myths of synthetic biology.</p> | <p>iGEM projects are not simply scientific works, but are also keen to involve and be relevant to the society. Our ethics and bioart exhibition activities provided insights, how fundamental projects, that deal with improving methods of construction of GMMs, should be presented and what concerns they elicit. Our human practices made us more aware and socially sensitive, and we integrated this approach to our project making it more public-friendly and open for discussion to debunk the fears and myths of synthetic biology.</p> |
Latest revision as of 01:46, 2 November 2017
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