Difference between revisions of "Team:Calgary/MedalRequirements"

 
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<img src="https://static.igem.org/mediawiki/2017/b/b3/Calgary2017_BronzeMadal.png">
 
<img src="https://static.igem.org/mediawiki/2017/b/b3/Calgary2017_BronzeMadal.png">
 
</div>
 
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<p>This page demonstrates a 3 column responsive layout, complete with responsive images and jquery slideshow.</p>
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<h2>BRONZE MEDAL</h2>
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<h3>Register and Attend</h3>
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<p>The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!</p>
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<h3>Deliverables</h3>
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<ul>
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<li><a href="https://2017.igem.org/Team:Calgary">Wiki</a></li>
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<li>Poster</li>
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<li>Presentation (come see us on Nov. 12, 11:30 am in Ballroom A)</li>
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<li><a href="https://2017.igem.org/Safety/Final_Safety_Form?team_id=2260">Safety Forms</a> (completed)</li>
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<li><a href="https://igem.org/2017_Judging_Form?id=2260">Judging Form</a> (completed)</li>
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<li>Registry Part Pages
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<br><ul><li><a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K2260001">BBa_K2260001</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a></li></ul></li>
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<li><a href="http://parts.igem.org/cgi/dna_transfer/one_batch.cgi?batch_id=4492">Sample Submission</a></li>
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</ul>
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<h3>Attributions</h3>
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<p>Check out our <a href="https://2017.igem.org/Team:Calgary/Attributions">Attributions</a> page for detailed information concerning the contributions of team members, mentors, and advisors!</p>
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<h3>Characterization/<br>Contribution</h3>
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<p>Our <a href="https://2017.igem.org/Team:Calgary/InterLab">InterLab</a> page shows how our team participated in the InterLab study this year!</p>
 
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<img src="https://static.igem.org/mediawiki/2017/5/5e/Calgary2017_SilverMedal.png">
 
<img src="https://static.igem.org/mediawiki/2017/5/5e/Calgary2017_SilverMedal.png">
 
</div>
 
</div>
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<h2>2nd Content Area</h2>
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<h2>SILVER MEDAL</h2>
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<p>At full width all three columns will be displayed side by side. As the page is resized the third column will collapse under the first and second. At the smallest screen size all three columns will be stacked on top of one another.</p>
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<p>Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum zzril delenit augue duis dolore te feugait nulla facilisi.</p>
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<h3>Validate a Part</h3>
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<p><a href="http://parts.igem.org/Part:BBa_K2260001">BBa_K2260001</a></p>
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<p>We submitted a new gene to the registry to assist in the production of PHB: the <i>phaJ</i> gene that codes for PHB enoyl-CoA hydratase. This part contains both the <i>phaJ</i> and <i>phaC</i> genes and was shown to produce PHB on its own in <i>E. coli</i> under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's <a href="http://parts.igem.org/Part:BBa_K2260001">Registry</a> page.</p>
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<h3>Collaboration</h3>
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<p>View our Canadian iGEM newsletter and wet-lab work with <a href="https://2017.igem.org/Team:McMasterU">iGEM McMasterU</a> on our <a href="https://2017.igem.org/Team:Calgary/Collaborations">Collaborations</a> page!</p>
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<h3>Human Practices Silver</h3>
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<p>We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns. See our <a href="https://2017.igem.org/Team:Calgary/HP/Silver">Human Practices Silver</a> page for more info!</p>
 
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<img src="https://static.igem.org/mediawiki/2017/5/5b/Calgary2017_GoldMedal.png">
 
<img src="https://static.igem.org/mediawiki/2017/5/5b/Calgary2017_GoldMedal.png">
 
</div>
 
</div>
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<h2>3rd Content Area</h2>
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<h2>GOLD MEDAL</h2>
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<p>Eodem modo typi, qui nunc nobis videntur parum clari, fiant sollemnes in futurum.</p>
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<h3>Integrated Human Practices</h3>
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<p>Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat.</p>
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<p>We continuously engaged in conversation with end-users, stakeholders, and experts in the field to inform the design and safety of our project. Find out more about our <a href="https://2017.igem.org/Team:Calgary/HP/Gold_Integrated">Integrated Human Practices</a>!</p>
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<p>Eodem modo typi, qui nunc nobis videntur parum clari, fiant sollemnes in futurum.</p>
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<h3>Modelling</h3>
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<p>Our team worked on two types of mathematical modelling: kinetic and flux balance. You can check out the details of that on our <a href="https://2017.igem.org/Team:Calgary/Model">Modelling</a> page.</p>
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<h3>Improve a Previous Part</h3>
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<p><a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a> and <a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a></p>
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<p>We improved two parts. The first, <a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a>, was an improvement on <a href="http://parts.igem.org/Part:BBa_K1149052">Imperial College's phaCAB operon</a> submitted in 2013. The gene order was changed to improve PHB yield (Hiroe <i>et al.</i>, 2012), codons were optimized for expression in <i>E. coli</i>, and restriction sites were removed to allow part compatibility in all iGEM RFC assembly standards. The second, <a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a>, was an improvement on <a href="http://parts.igem.org/Part:BBa_K2018024">SDU Denmark's phasin-HlyA</a> part submitted in 2016. Codons were optimized for expression in <i>E. coli</i>.</p>
 
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<h2>Works Cited</h2>
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<p>Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184</p>
  
 
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Latest revision as of 02:09, 2 November 2017

Header

Judging Medal Requirements

BRONZE MEDAL

Register and Attend

The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!

Deliverables

Attributions

Check out our Attributions page for detailed information concerning the contributions of team members, mentors, and advisors!

Characterization/
Contribution

Our InterLab page shows how our team participated in the InterLab study this year!

SILVER MEDAL

Validate a Part

BBa_K2260001

We submitted a new gene to the registry to assist in the production of PHB: the phaJ gene that codes for PHB enoyl-CoA hydratase. This part contains both the phaJ and phaC genes and was shown to produce PHB on its own in E. coli under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's Registry page.

Collaboration

View our Canadian iGEM newsletter and wet-lab work with iGEM McMasterU on our Collaborations page!

Human Practices Silver

We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns. See our Human Practices Silver page for more info!


Works Cited

Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184