Difference between revisions of "Team:Calgary/MedalRequirements"

 
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<h3>Register and Attend</h3>
 
<h3>Register and Attend</h3>
 +
<p>The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!</p>
 
<h3>Deliverables</h3>
 
<h3>Deliverables</h3>
 +
<ul>
 +
<li><a href="https://2017.igem.org/Team:Calgary">Wiki</a></li>
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<li>Poster</li>
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<li>Presentation (come see us on Nov. 12, 11:30 am in Ballroom A)</li>
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<li><a href="https://2017.igem.org/Safety/Final_Safety_Form?team_id=2260">Safety Forms</a> (completed)</li>
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<li><a href="https://igem.org/2017_Judging_Form?id=2260">Judging Form</a> (completed)</li>
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<li>Registry Part Pages
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<br><ul><li><a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K2260001">BBa_K2260001</a></li>
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<li><a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a></li></ul></li>
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<li><a href="http://parts.igem.org/cgi/dna_transfer/one_batch.cgi?batch_id=4492">Sample Submission</a></li>
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</ul>
 
<h3>Attributions</h3>
 
<h3>Attributions</h3>
−
<h3>Characterization/Contribution</h3>
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<p>Check out our <a href="https://2017.igem.org/Team:Calgary/Attributions">Attributions</a> page for detailed information concerning the contributions of team members, mentors, and advisors!</p>
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<p>"Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."</p>
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<h3>Characterization/<br>Contribution</h3>
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<p>Our <a href="https://2017.igem.org/Team:Calgary/InterLab">InterLab</a> page shows how our team participated in the InterLab study this year!</p>
 
</div>
 
</div>
 
 
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<h2>SILVER MEDAL</h2>
 
<h2>SILVER MEDAL</h2>
  
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<h4>Validate a Part</h4>
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<h3>Validate a Part</h3>
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<h4>Collaboration</h4>
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<p><a href="http://parts.igem.org/Part:BBa_K2260001">BBa_K2260001</a></p>
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<h4>Human Practices</h4>
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<p>We submitted a new gene to the registry to assist in the production of PHB: the <i>phaJ</i> gene that codes for PHB enoyl-CoA hydratase. This part contains both the <i>phaJ</i> and <i>phaC</i> genes and was shown to produce PHB on its own in <i>E. coli</i> under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's <a href="http://parts.igem.org/Part:BBa_K2260001">Registry</a> page.</p>
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<p>Woo fun medal requriements!.</p>
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<h3>Collaboration</h3>
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<p>View our Canadian iGEM newsletter and wet-lab work with <a href="https://2017.igem.org/Team:McMasterU">iGEM McMasterU</a> on our <a href="https://2017.igem.org/Team:Calgary/Collaborations">Collaborations</a> page!</p>
 +
<h3>Human Practices Silver</h3>
 +
<p>We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns. See our <a href="https://2017.igem.org/Team:Calgary/HP/Silver">Human Practices Silver</a> page for more info!</p>
 
</div>
 
</div>
  
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</div>
 
</div>
 
<h2>GOLD MEDAL</h2>
 
<h2>GOLD MEDAL</h2>
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<h4>Integrated Human Practices</h4>
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<h3>Integrated Human Practices</h3>
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<h4>Modelling</h4>
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<p>We continuously engaged in conversation with end-users, stakeholders, and experts in the field to inform the design and safety of our project. Find out more about our <a href="https://2017.igem.org/Team:Calgary/HP/Gold_Integrated">Integrated Human Practices</a>!</p>
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<p>Eodem modo typi, qui nunc nobis videntur parum clari, fiant sollemnes in futurum.</p>
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<h3>Modelling</h3>
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<p>Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat.</p>
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<p>Our team worked on two types of mathematical modelling: kinetic and flux balance. You can check out the details of that on our <a href="https://2017.igem.org/Team:Calgary/Model">Modelling</a> page.</p>
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<p>Eodem modo typi, qui nunc nobis videntur parum clari, fiant sollemnes in futurum.</p>
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<h3>Improve a Previous Part</h3>
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<p><a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a> and <a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a></p>
 +
<p>We improved two parts. The first, <a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a>, was an improvement on <a href="http://parts.igem.org/Part:BBa_K1149052">Imperial College's phaCAB operon</a> submitted in 2013. The gene order was changed to improve PHB yield (Hiroe <i>et al.</i>, 2012), codons were optimized for expression in <i>E. coli</i>, and restriction sites were removed to allow part compatibility in all iGEM RFC assembly standards. The second, <a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a>, was an improvement on <a href="http://parts.igem.org/Part:BBa_K2018024">SDU Denmark's phasin-HlyA</a> part submitted in 2016. Codons were optimized for expression in <i>E. coli</i>.</p>
 
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<p>Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184</p>
  
 
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Latest revision as of 02:09, 2 November 2017

Header

Judging Medal Requirements

BRONZE MEDAL

Register and Attend

The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!

Deliverables

Attributions

Check out our Attributions page for detailed information concerning the contributions of team members, mentors, and advisors!

Characterization/
Contribution

Our InterLab page shows how our team participated in the InterLab study this year!

SILVER MEDAL

Validate a Part

BBa_K2260001

We submitted a new gene to the registry to assist in the production of PHB: the phaJ gene that codes for PHB enoyl-CoA hydratase. This part contains both the phaJ and phaC genes and was shown to produce PHB on its own in E. coli under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's Registry page.

Collaboration

View our Canadian iGEM newsletter and wet-lab work with iGEM McMasterU on our Collaborations page!

Human Practices Silver

We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns. See our Human Practices Silver page for more info!


Works Cited

Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184