Difference between revisions of "Team:Calgary/MedalRequirements"

 
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<h3>Register and Attend</h3>
 
<h3>Register and Attend</h3>
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The team will be attending Boston in November!
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<p>The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!</p>
 
<h3>Deliverables</h3>
 
<h3>Deliverables</h3>
 
<ul>
 
<ul>
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<li>Wiki</li>
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<li><a href="https://2017.igem.org/Team:Calgary">Wiki</a></li>
 
<li>Poster</li>
 
<li>Poster</li>
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<li>Presentation</li>
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<li>Presentation (come see us on Nov. 12, 11:30 am in Ballroom A)</li>
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<li>Safety Forms</li>
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<li><a href="https://2017.igem.org/Safety/Final_Safety_Form?team_id=2260">Safety Forms</a> (completed)</li>
−
<li>Judging Form</li>
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<li><a href="https://igem.org/2017_Judging_Form?id=2260">Judging Form</a> (completed)</li>
−
<li>Registry Part Pages</li>
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<li>Registry Part Pages
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<li>Project Attributions</li>
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<br><ul><li><a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a></li>
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<li>Sample Submissions</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2260001">BBa_K2260001</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a></li></ul></li>
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<li><a href="http://parts.igem.org/cgi/dna_transfer/one_batch.cgi?batch_id=4492">Sample Submission</a></li>
 
</ul>
 
</ul>
 
<h3>Attributions</h3>
 
<h3>Attributions</h3>
−
<p>Check out our <a href="https://2017.igem.org/Team:Calgary/Attributions">Attributions</a> page for detailed info about who worked on which parts of our project!</p>
+
<p>Check out our <a href="https://2017.igem.org/Team:Calgary/Attributions">Attributions</a> page for detailed information concerning the contributions of team members, mentors, and advisors!</p>
−
<h3>Characterization/Contribution</h3>
+
<h3>Characterization/<br>Contribution</h3>
−
<p>Check out our <a href="https://2017.igem.org/Team:Calgary/InterLab">InterLab</a> page to see the way our team participated in the InterLab study this year!</p>
+
<p>Our <a href="https://2017.igem.org/Team:Calgary/InterLab">InterLab</a> page shows how our team participated in the InterLab study this year!</p>
 
</div>
 
</div>
 
 
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<h3>Validate a Part</h3>
 
<h3>Validate a Part</h3>
 +
<p><a href="http://parts.igem.org/Part:BBa_K2260001">BBa_K2260001</a></p>
 +
<p>We submitted a new gene to the registry to assist in the production of PHB: the <i>phaJ</i> gene that codes for PHB enoyl-CoA hydratase. This part contains both the <i>phaJ</i> and <i>phaC</i> genes and was shown to produce PHB on its own in <i>E. coli</i> under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's <a href="http://parts.igem.org/Part:BBa_K2260001">Registry</a> page.</p>
 +
 
<h3>Collaboration</h3>
 
<h3>Collaboration</h3>
−
<p>More info about our collaborations can be found <a href="https://2017.igem.org/Team:Calgary/Collaborations">here!</a></p>
+
<p>View our Canadian iGEM newsletter and wet-lab work with <a href="https://2017.igem.org/Team:McMasterU">iGEM McMasterU</a> on our <a href="https://2017.igem.org/Team:Calgary/Collaborations">Collaborations</a> page!</p>
 
<h3>Human Practices Silver</h3>
 
<h3>Human Practices Silver</h3>
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Info about Human Practices Here
+
<p>We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns. See our <a href="https://2017.igem.org/Team:Calgary/HP/Silver">Human Practices Silver</a> page for more info!</p>
 
</div>
 
</div>
  
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<h2>GOLD MEDAL</h2>
 
<h2>GOLD MEDAL</h2>
 
<h3>Integrated Human Practices</h3>
 
<h3>Integrated Human Practices</h3>
 +
<p>We continuously engaged in conversation with end-users, stakeholders, and experts in the field to inform the design and safety of our project. Find out more about our <a href="https://2017.igem.org/Team:Calgary/HP/Gold_Integrated">Integrated Human Practices</a>!</p>
 
<h3>Modelling</h3>
 
<h3>Modelling</h3>
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<p>Our team worked on two types of mathematical modelling: kinetic and flux balance. You can check out the details of that on our <a href="https://2017.igem.org/Team:Calgary/Modelling">Modelling</a> page.</p>
+
<p>Our team worked on two types of mathematical modelling: kinetic and flux balance. You can check out the details of that on our <a href="https://2017.igem.org/Team:Calgary/Model">Modelling</a> page.</p>
 +
<h3>Improve a Previous Part</h3>
 +
<p><a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a> and <a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a></p>
 +
<p>We improved two parts. The first, <a href="http://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a>, was an improvement on <a href="http://parts.igem.org/Part:BBa_K1149052">Imperial College's phaCAB operon</a> submitted in 2013. The gene order was changed to improve PHB yield (Hiroe <i>et al.</i>, 2012), codons were optimized for expression in <i>E. coli</i>, and restriction sites were removed to allow part compatibility in all iGEM RFC assembly standards. The second, <a href="http://parts.igem.org/Part:BBa_K2260002">BBa_K2260002</a>, was an improvement on <a href="http://parts.igem.org/Part:BBa_K2018024">SDU Denmark's phasin-HlyA</a> part submitted in 2016. Codons were optimized for expression in <i>E. coli</i>.</p>
 
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|REFERENCES=
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<h2>Works Cited</h2>
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<p>Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184</p>
  
 
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Latest revision as of 02:09, 2 November 2017

Header

Judging Medal Requirements

BRONZE MEDAL

Register and Attend

The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!

Deliverables

Attributions

Check out our Attributions page for detailed information concerning the contributions of team members, mentors, and advisors!

Characterization/
Contribution

Our InterLab page shows how our team participated in the InterLab study this year!

SILVER MEDAL

Validate a Part

BBa_K2260001

We submitted a new gene to the registry to assist in the production of PHB: the phaJ gene that codes for PHB enoyl-CoA hydratase. This part contains both the phaJ and phaC genes and was shown to produce PHB on its own in E. coli under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's Registry page.

Collaboration

View our Canadian iGEM newsletter and wet-lab work with iGEM McMasterU on our Collaborations page!

Human Practices Silver

We carefully weighed the effects our project could have in a variety of applications, and considered possible benefits, challenges, and safety concerns. See our Human Practices Silver page for more info!


Works Cited

Hiroe, A., Tsuge, K., Nomura, C.T., Itaya, M. & Tsuge, T. (2012). Rearrangement of Gene Order in the phaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli. Appl. Environ. Microbiol., 78(9): 3177-3184