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             <h1>Model</h1>
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             <h2>Development of A Novel Blood-MicroRNA Handy Detection System with CRISPR</h2>
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      <li id="abstract" class="liList">
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            <div class="myheader"id="main"><h1>MiRNA &nbsp;&nbsp;Locker</h1><h3>A Modularized DNA Assembly As miRNA Inhibitors</h3><button id="btn1" class="button-next">How it works?</button></div>
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            <h2 class="content-subhead">Abstract</h2>
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            <p>
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                This model is created to evaluate the effectiveness of initial design, and offers guidelines on how the system can (or must) be improved. (You can go to <a href="https://2016.igem.org/Team:NUDT_CHINA/Design">PROJECT. page</a> to see more
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            </p>
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            <h2 class="content-subhead">Introduction</h2>
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                We create mathematical models of two aspects of our project, a RCA model and a signal detection model.
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            <h2 class="content-subhead">Assumption and Justification</h2>
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            <h2 class="content-subsub">About model </h2>
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            <p>1. MiRNA is not degraded throughout the reaction process.</p>
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<p>2. The two fusion proteins of dCas9 and split-HRP fragments have the same ability to combine with the stem-loop structure, and only when two different proteins next to each other, can they have the ability to catalyze substrate and produce signal.</p>
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<p>3. The number of stem-loop structures in each RCA product is equal under a certain reaction time.</p>
+
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<p>4. The enzymatic activity remains unchanged with time under the premise of excessive amount of enzymes or a short-time reaction. </p>
+
−
            <h2 class="content-subsub">About the data </h2>
+
−
            <p>1. The data we obtain from wet-lab experiment are reliable.</p>
+
−
<p>2. All the results are trustworthy in the process of statistical processing and data calculation.</p>
+
−
 
+
−
            <h2 class="content-subhead">Model</h2>
+
−
            <h2 class="content-subsub">Notations</h2>
+
−
           
+
−
            <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" style="border:none;">
+
−
<tbody>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">Symbol </span>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">Definition </span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">x</span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK7"></a><a name="OLE_LINK6"></a><span style="font-family:&quot;">The
+
−
  concentration of</span><span style="font-family:&quot;"> miRNA (pM)</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">C<sub>1</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The
+
−
  concentration of initiated probe (Abbreviated to iprobe)</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">k<sub>1</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK8"></a><span style="font-family:&quot;">A constant representing the scale factor</span><span style="font-family:&quot;"></span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">K<sub>m </sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">One
+
−
  of the characteristic constants of phi29 DNA polymerase</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">V<sub>max</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">One
+
−
  of the characteristic constants of phi29 DNA polymerase</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">k<sub>2</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">A
+
−
  constant representing the scale factor</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">V</span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The initial
+
−
  speed of RCA </span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK9"></a><i><span style="font-family:&quot;">n<sub>1</sub></span></i><i><sub><span style="font-family:&quot;"></span></sub></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The
+
−
  moles of RCA product</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">n<sub>2</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK10"></a><span style="font-family:&quot;">The number of stem-loop structures in each RCA product</span><span style="font-family:&quot;"></span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">n</span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The total
+
−
  amount of <a name="OLE_LINK23"></a><a name="OLE_LINK22"></a>stem-loop structures</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">N</span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK41"></a><a name="OLE_LINK16"></a><span style="font-family:&quot;">The
+
−
  molecule number of the fusion protein of dCas9 and split-HRP fragments</span><span style="font-family:&quot;"></span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">k<sub>3</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">A
+
−
  constant representing the scale factor</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">y<sub>1</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The fluorescence
+
−
  intensity of DNA-dye-complex (RFU)</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">N<sub>1</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The
+
−
  molecule number of the fusion protein of dCas9 and split-HRP fragments in the
+
−
  solution</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">N<sub>2</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK45"></a><span style="font-family:&quot;">The molecule number of the fusion protein of dCas9 and
+
−
  split-HRP fragments binding with stem-loop structure</span><span style="font-family:&quot;"></span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">k<sub>4</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">A
+
−
  constant representing the scale factor</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">k<sub>5</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">A
+
−
  constant representing the scale factor</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">ρ</span></i><i><span style="font-family:&quot;"></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">Signal
+
−
  to noise ratio(Abbreviated to SNR)</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">I</span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The
+
−
  molecule number of formed intact HRP proteins </span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">I<sub>1</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The
+
−
  molecule number of formed intact HRP proteins in the solution</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">I<sub>2</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<a name="OLE_LINK43"></a><span style="font-family:&quot;">The molecule number of formed intact HRP proteins through
+
−
  binding with stem-loop structure</span><span style="font-family:&quot;"></span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">t</span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">Reaction
+
−
  time</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
<tr>
+
−
<td width="66" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<i><span style="font-family:&quot;">y<sub>2</sub></span></i>
+
−
</p>
+
−
</td>
+
−
<td width="487" valign="top" style="border:solid windowtext 1.0pt;">
+
−
<p class="MsoNormal">
+
−
<span style="font-family:&quot;">The
+
−
  signal intensity (OD<sub>450</sub>)</span>
+
−
</p>
+
−
</td>
+
−
</tr>
+
−
</tbody>
+
−
</table>
+
−
<div class="pure-g">
+
−
                  <div class="pure-u-1-1">
+
−
                    <img class="pure-img-responsive" src="show_test.jpg" alt="Peyto Lake">
+
−
                </div>
+
−
                </div>
+
−
                <p><br>
+
−
<span style="line-height:2;font-family:Perpetua;font-
+
−
 
+
−
size:18px;"><b>Figure 1. Schematic diagram</b></span>
+
−
</p>
+
−
 
+
−
     
+
−
        </div>
+
−
    </div>
+
−
 
+
−
</div>
+
−
 
+
 
</div>
 
</div>
 
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Latest revision as of 02:17, 2 November 2017

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