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<h2>Market Analysis</h2>
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<h2>Apply for Best Composite Part</h2>
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<p>This year, the final goal of iGEM_Bit team is to provide the potential versatility, precision, and affordability of JACOB—essentially a representation of modularized biomarker detections—to ordinary communities.  
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<p><h3>This year we design our whole part including strong promoter, Cyclic Amplifier and Dual Reporter fluorescence, We also building 19 new composite parts and we choose the best functional part to apply for this prize.<br>
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Our plan is to commercialize JACOB to provide warnings for potential disease and develop a self-sustaining facility for production and further research.
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The BBa_K2305004 is strong constitutive promoter pcat and plux promoter with GFP, we increase the value of fluorescence above 10 times to the normal plux promoter with GFP. For the results of the characterizations please click<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2305004"> BBa_2305004</a>.</h3></p>
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From market analysis, we concluded the prominent advantages of JACOB and focused on those to provide more competitive products; from direct interaction with commercial industries, we optimized JACOB's operability and compatibility with the market.
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<h2>Wireframe Connects the Underlying Conceptual Structure</h2>
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<h3>Wireframe Connects the Underlying Conceptual Structure</h3>
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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<span>Louie Jie Mahusay</span><br>
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<small>CEO, Founder</small>
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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<h2>Market Analysis</h2>
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<h3>Modeling of Promoter Start Efficiency for Complex Part</h3>
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<p><h3>The expression formula of promoter activity was constructed by Hill equation. β1 is the maximum starting ability of the promoter (generally set to 1), M1 is the amount of leakage of the promoter, SluxR is the amount of regulation factor, kd is the SluxR value when the model change trend is changed:</h3><br>
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<p><span class="label-warning">a. Four Aspects:</span><br>
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  <span class="lead">&nbsp;&nbsp;
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  i. Strength:
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  </span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; 1. Convenience.
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  <br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2. Adaptability.
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; 3. High Cost/Performance Ratio.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;
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  4. Inelastic to change in test conditions.<br>
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  <span class="lead">&nbsp;&nbsp;
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  ii. Weakness:</span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  1. Technology Barrier.
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  2. Branding Barrier.
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  3. Strict Market Admittance Criteria.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  4. Distribution Barrier.<br>
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  <span class="lead">&nbsp;&nbsp;
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  iii. Opportunity:</span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  1. Favorable national economic condition.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  2. Positive national policies.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  3. Uprising Health-consciousness.<br>
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  <span class="lead">&nbsp;&nbsp;
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  iv. Threats:</span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;
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  1. Well-established industries control the high-end markets with related products.
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<br>
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  &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
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  2. Industrial chain is currently complete
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<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;
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  3. Technological applications are matured</p>
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  <p> <span class="label-warning">b. Comments: </span><br>
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&nbsp;&nbsp; Comparing to technology equipped by clinics and hospitals, The use of adjustable aptamer and genetic modified microbes made JACOB more economical, adaptable, and stable. The integration of automated proceeding made JACOB more user-friendly and portable. JACOB does not need professional physicians reading results, behaving like glucose meter but less risky. In China it is likely to be deployed in units of household or local communities. Similarly, for United States the Food and Drug Administration classified the core mechanism of JACOB as Class I medical device (if designed to not detecting cancer biomarkers), means no premarketing control is required. It is worth noting though, Chinese in vitro diagnostic market is relatively new and have few competitors. Those few players can no longer support the concrete increasing demand for medical cares. Chinese government had ratified a great number of acts as well as guidelines and simplified documents examining and approval process to encourage innovation in the field of medical devices, which offers our team a great market environment. </p>
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<h2>Wireframe Connects the Underlying Conceptual Structure</h2><p>In market analysis we had solved many issues regarding our project background, but also generated more questions about operability. To obtain a better answer, we had visited 北京永翰星港生物技术有限公司和博奥生物芯片公司. </p>
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<p>We asked 蓝晶 mainly on how to construct a viable procedure to industrialize JACOB. Our plan for JACOB after the iGEM jamboree is divided into developments, commercialization, and resource transformation. For development we currently divided it into small, medium, large scale trial. Based on our current knowledge, small and medium trial is planned as below  </p>
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<span>Louie Jie Mahusay</span><br>
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<small>CEO, Founder</small>
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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<p><span class="label-warning">1. Small Scale Trial</span><br>
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                    &nbsp;&nbsp;&nbsp; a. Duration: About half year
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                    &nbsp;&nbsp;&nbsp; b. We will choose the production method for JACOB, e.g. as we learned from 永翰星港, the "filtration" of aptamers are still expensive, but aptamers synthesis is more economical. Nine sets of known aptamer (90np, 15 microliter, 100nmol/L) costs only about $135, and every set is sufficient for 250 microfluidic chips.<br>
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                    &nbsp;&nbsp;&nbsp;
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c. During this phase we also need to rule out unknown influences on the device caused by switching to new assembly conditions, such as switching to fermentation flask instead of tightly controlled experiment chamber.
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<br>
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&nbsp;&nbsp;&nbsp;
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d. During this phase preserving microbes will greatly affect the cost of production, and affordability is one of the most important characteristics of JACOB.<br>
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<span class="label-warning">2. Medium Scale Trial</span><br>
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&nbsp;&nbsp;&nbsp;
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a. Duration: 4-5 months<br>
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&nbsp;&nbsp;&nbsp;
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b. When the production of microfluidic chip is resolved, how to prepare it for using will be on the agenda. Currently the chip we used for final packaging needs to be soaked in ethyl alcohol and acetone for about one hour, which pose a question for mass production. </p>
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<p>We asked 博奥 mainly on how to control the cost of microfluidic chip production¬. From their experience we found that chips cost much more in the early development, but its cost diminishes quickly in later stages when mass production is reached, ranging from $0.1 to few dollars. However, there exist a conflict of material. In the iGEM version we picked optical cement instead of PMDS for its advantages of adequate solidification speed, feasibility, bio-inertia, and precision of altitude. However, PMDS production industries are much more matured. There is currently no companies offering mass production of optical cement microfluidic chips. Thus, we must decide the better material.</p>
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Among them, according to the database in the official website of igem , we can know the value of some parameters: the maximum capacity of start-up promoterβ1 = 0.6; the amount of the leakage of promoter M1 = 0; n1 = 2.<br><center>
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So we put these known parameters into the equation and get the value of kd at equal distances: 50,100,150,200, finally draw a rough image:<br>
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Latest revision as of 02:27, 2 November 2017

Dsign - Minimal portfolio Bootstrap template

Apply for Best Composite Part

This year we design our whole part including strong promoter, Cyclic Amplifier and Dual Reporter fluorescence, We also building 19 new composite parts and we choose the best functional part to apply for this prize.
The BBa_K2305004 is strong constitutive promoter pcat and plux promoter with GFP, we increase the value of fluorescence above 10 times to the normal plux promoter with GFP. For the results of the characterizations please click BBa_2305004.

Modeling of Promoter Start Efficiency for Complex Part

The expression formula of promoter activity was constructed by Hill equation. β1 is the maximum starting ability of the promoter (generally set to 1), M1 is the amount of leakage of the promoter, SluxR is the amount of regulation factor, kd is the SluxR value when the model change trend is changed:


Among them, according to the database in the official website of igem , we can know the value of some parameters: the maximum capacity of start-up promoterβ1 = 0.6; the amount of the leakage of promoter M1 = 0; n1 = 2.
So we put these known parameters into the equation and get the value of kd at equal distances: 50,100,150,200, finally draw a rough image:

Contact Us!

Please keep reading if you want to contact us.