Difference between revisions of "Team:Kyoto/Attributions"

 
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        <li><a href="https://2017.igem.org/Team:Kyoto">Home</a></li>
 
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        <li><a href="https://2017.igem.org/team:Kyoto/Project">Project</a>
 
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            <ul>
 
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              <li><a href="https://2017.igem.org/team:Kyoto/Project">Description</a></li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Results">Results</a></li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Demonstrate">Demonstrate</li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Basic_Part">Basic parts</a></li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Composite_Part">Composite parts</a></li>
 
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            </ul>
 
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        </li><li><a href="https://2017.igem.org/Team:Kyoto/Experiments">Material&amp;Methods</a></li><li><a href="https://2017.igem.org/Team:Kyoto/Achievements">Medal criteria</a>
 
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            <ul>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Achievements">Achievements</a></li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/HP/Silver">HP/Silver</a></li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/HP/Gold_Integrated">HP/Gold</a></li>
 
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            </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li><li><a href="https://2017.igem.org/Team:Kyoto/integrated">Practice</a>
 
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            <ul>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Human_Practices">Human Practice</li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Integrated_Practices">Integrated Human Practice</li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Engagement">Public Engagement</li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Collaborations">Collaboration</li>
 
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            </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a>
 
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            <ul>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Team">Members</li>
 
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              <li><a href="https://2017.igem.org/Team:Kyoto/Attributions">Attributions</li>
 
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            </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li>
 
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      </ul>
 
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   <h1>Attributions</h1>
 
   <h1>Attributions</h1>
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<h4>
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   <h5>■Instructors</h5>
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We succeeded in culturing <i>B. Xyphilus</i> by <i>S. Cerevisiae</i> to establish the system of inducing RNAi by feeding. In addition, we transformed the plasmid coding hairpin-dsRNA to <i>S. Cerevisiae</i>, fed it to <i>B. Xyphilus</i>, and observed it.
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       <p><ul class="attribution">
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</h4>
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<li>Dr. Makoto Kitabatake (Institute for Frontier Life and Medical Sciences, Kyoto University) helped us all through our project, provided us with a laboratory, and gave us professional advice as well as training us on the experiment of <i>S. cerevisiae</i> and RNA. In addition, he helped collect and analysis experiment data, and reform our wiki writing. He also gave us safety education and ethics education.<li>Dr. Knut Woltjen (Center of iPS Cell Research and Application, and Hakubi Center for Advanced Research, Kyoto University) helped us all through our project, and gave us professional advice.</li>
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   <h5>■General support</h5>
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       <p><ul>
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<li>Dr. Makoto Kitabatake (Institute for Virus Research, Kyoto University) helped us all through our project, provided us with a laboratory, and gave us professional advice as well as training us on the experiment of <i>S. Cerevisiae</i> and RNA.</li>　
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−
<br>
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<li>Dr. Knut Woltzen (Center of iPS Cell Research and Application, Kyoto University) helped us all through our project, and gave us professional advice.</li>
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       </ul></p>
 
       </ul></p>
  
 
   <h5>■Project support and advice</h5>
 
   <h5>■Project support and advice</h5>
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       <p><ul>
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       <p><ul class="attribution">
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<li>Dr. Yuko Takeuchi (Department of Agriculture, Kyoto University) provided us with <i>B. Xylophilus</i>, trained us on culturing and observing it ,and also provided us with a laboratory. </li>
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<li>Dr. Yuko Takeuchi (Graduate School of Agriculture, Kyoto University) provided us with <i>B. xylophilus</i>, trained us on culturing and observing it, and also provided us with laboratory facilities. </li>
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<br>
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<li>Mr. Suguru E. Tanaka (Graduate School of Agricultural and Life Sciences, The University of Tokyo) trained us on culturing <I>B. xylophilus</I>, and provided professional advice on the experiments</li>
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<li>Mr. Suguru Tanaka (Department of Agriculture, the University of Tokyo) trained us on culturing <i>B. Xylophilus</i>, and gave us a professional advice on it.</li>
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       </ul></p>
 
       </ul></p>
  
 
   <h5>■Fundraising help and advice</h5>
 
   <h5>■Fundraising help and advice</h5>
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       <p><ul>
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       <p><ul class="attribution">
 
<li>COSMO BIO provided us with reagents and kits, free of charge.</li>
 
<li>COSMO BIO provided us with reagents and kits, free of charge.</li>
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<br>
 
 
<li>Promega Corporation provided us with regents and kits, free of charge.</li>
 
<li>Promega Corporation provided us with regents and kits, free of charge.</li>
 
       </ul></p>
 
       </ul></p>
  
 
   <h5>■Lab support</h5>
 
   <h5>■Lab support</h5>
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       <p><ul>
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       <p><ul class="attribution">
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<li>Prof. Teiji Sota (Department of Science, Kyoto University) helped us with the usage of laboratory, provided us with a laboratory ,and let us use -80℃ freezer and Nanodrop of his laboratory.</li>
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<li>Prof. Teiji Sota (Department of Science, Kyoto University) helped us with the usage of laboratory, provided us with a laboratory, and let us use -80℃ freezer, the microscopy and Nanodrop of his laboratory.</li>
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<br>
+
 
<li>Dr. Tomoo Shimada (Department of Science, Kyoto University) provided us with a laboratory.</li>
 
<li>Dr. Tomoo Shimada (Department of Science, Kyoto University) provided us with a laboratory.</li>
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<br>
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<li>Dr. Shuhei Nasuda (Graduate School of Agriculture, Kyoto University) provided us with a laboratory and a meeting room. </li>
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<li>Dr. Shuhei Nasuda (Department of Science, Kyoto University) provided us with a laboratory and a meeting room and provided us with some regents.</li>
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       </ul></p>
 
       </ul></p>
  
 
   <h5>■Difficult technique support</h5>
 
   <h5>■Difficult technique support</h5>
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       <p><ul>
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       <p><ul class="attribution">
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<li>Dr. Ichiro Taniguchi (Institute for Virus Research, Kyoto University) provided us with the background of RNA nuclear export and helped us with the microinjection RI-labeled RNA into xenopus oocytes and the analysis. In addition, he provided us with DNA coding Rev protein.</li> 　
+
<li>Dr. Ichiro Taniguchi (Institute for Frontier Life and Medical Sciences, Kyoto University) provided us with the background of RNA nuclear export and helped us with the microinjection of RI-labeled RNA into Xenopus oocytes and the analysis. In addition, he provided us with DNA coding Rev protein.</li> 　
−
<br>
+
<li>Dr. Ryusuke Tadokoro (Division of Biological Science, Graduate School of Science, Kyoto University) helped us with the usage of a confocal microscopy.
−
<li>Dr. Ryusuke Tadokoro (Department of Science, Kyoto University) helped us with the usage of a confocal microscopy.  
+
 
       </ul></p>
 
       </ul></p>
  
 
   <h5>■Wiki support and presentation coaching</h5>
 
   <h5>■Wiki support and presentation coaching</h5>
−
       <p><ul>
+
       <p><ul class="attribution">
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<li>James-Alan Hejna (Graduated School of Biostudies, Kyoto University) gave us valuable advice about our presentation and wiki.</li>
+
<li>James Hejna(Graduated School of Biostudies, Kyoto University) gave us valuable advice about our presentation and wiki.</li>
 +
      <li>Mr. Hideaki Tsujii (Faculty of medicine, Kyoto University) helped us with HTML codes for our wiki.</li>
 
       </ul></p>
 
       </ul></p>
  
 
   <h5>■Human Practice support</h5>
 
   <h5>■Human Practice support</h5>
−
       <p><ul>
+
       <p><ul class="attribution">
−
<li>Kyoto city （Mr. Takubo ）</li>
+
<li>Kyoto city </li>
−
<br>
+
<li>Kyoto city forestry cooperative（Ms. Takubo ）</li>
−
<li>Kyoto city forestry cooperative</li>
+
−
<br>
+
 
<li>Kyoto-Osaka forestry cooperative (Mr. Onishi, Mr. Enotani)</li>
 
<li>Kyoto-Osaka forestry cooperative (Mr. Onishi, Mr. Enotani)</li>
 
       </ul></p>
 
       </ul></p>
  
 
   <h5>■Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</h5>
 
   <h5>■Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</h5>
−
       <p><ul>
+
       <p><ul class="attribution">
 
<li>Prof. Keiko Kuroda (Department of Agriculture, Kobe University) provided us with  a background on the matter of the pine disease in Japan.</li>
 
<li>Prof. Keiko Kuroda (Department of Agriculture, Kobe University) provided us with  a background on the matter of the pine disease in Japan.</li>
−
<br>
+
<li>Dr. Taisei Kikuchi (Department of Medicine, Miyazaki University) suggested the possibility of culturing <i>B. xylophilus</i> with <i>S. cerevisiae</i>.</li>
−
<li>Dr. Taisei Kikuchi (Department of Medicine, Miyazaki University) suggested the possibility of culturing <i>B. Xylophilus</i> with <i>S. Cerevisiae</i>.</li>
+
<li>Mr. Shuhei Yamada (Undergraduate school of Engineering, Kyoto University) helped us with revising our English in wiki and presentation.</li>
−
<br>
+
−
<li>Mr. Shuhei Yamada (Underraduate school of Engineering, Kyoto University) helped us with revising our English in wiki and presentation.</li>
+
−
<br>
+
 
<li>Dr. Makoto Kashima (Department of Agriculture, Ryukoku University) helped us all through our projects.</li>
 
<li>Dr. Makoto Kashima (Department of Agriculture, Ryukoku University) helped us all through our projects.</li>
−
<br>
+
<li>Mr. Fumitaka Hashiya (Graduate School of Science, Kyoto University) helped us with the registration and storage of reagents.</li>
−
<li>Mr. Fumitaka Hashiya (Undergaduate School of Science, Kyoto University) helped us with the registration and storage of reagents.</li>
+
−
<br>
+
 
<li>Mr. Tomoki Uchino (Undergraduate School of Agriculture, Kyoto University) trained us on the cloning experiment.</li>
 
<li>Mr. Tomoki Uchino (Undergraduate School of Agriculture, Kyoto University) trained us on the cloning experiment.</li>
 
     </ul></p>
 
     </ul></p>
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 +
<br>
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Latest revision as of 02:31, 2 November 2017

Attributions

■Instructors

  • Dr. Makoto Kitabatake (Institute for Frontier Life and Medical Sciences, Kyoto University) helped us all through our project, provided us with a laboratory, and gave us professional advice as well as training us on the experiment of S. cerevisiae and RNA. In addition, he helped collect and analysis experiment data, and reform our wiki writing. He also gave us safety education and ethics education.
  • Dr. Knut Woltjen (Center of iPS Cell Research and Application, and Hakubi Center for Advanced Research, Kyoto University) helped us all through our project, and gave us professional advice.

■Project support and advice

  • Dr. Yuko Takeuchi (Graduate School of Agriculture, Kyoto University) provided us with B. xylophilus, trained us on culturing and observing it, and also provided us with laboratory facilities.
  • Mr. Suguru E. Tanaka (Graduate School of Agricultural and Life Sciences, The University of Tokyo) trained us on culturing B. xylophilus, and provided professional advice on the experiments

■Fundraising help and advice

  • COSMO BIO provided us with reagents and kits, free of charge.
  • Promega Corporation provided us with regents and kits, free of charge.

■Lab support

  • Prof. Teiji Sota (Department of Science, Kyoto University) helped us with the usage of laboratory, provided us with a laboratory, and let us use -80℃ freezer, the microscopy and Nanodrop of his laboratory.
  • Dr. Tomoo Shimada (Department of Science, Kyoto University) provided us with a laboratory.
  • Dr. Shuhei Nasuda (Graduate School of Agriculture, Kyoto University) provided us with a laboratory and a meeting room.

■Difficult technique support

  • Dr. Ichiro Taniguchi (Institute for Frontier Life and Medical Sciences, Kyoto University) provided us with the background of RNA nuclear export and helped us with the microinjection of RI-labeled RNA into Xenopus oocytes and the analysis. In addition, he provided us with DNA coding Rev protein.
    • 　
  • Dr. Ryusuke Tadokoro (Division of Biological Science, Graduate School of Science, Kyoto University) helped us with the usage of a confocal microscopy.

■Wiki support and presentation coaching

  • James Hejna(Graduated School of Biostudies, Kyoto University) gave us valuable advice about our presentation and wiki.
  • Mr. Hideaki Tsujii (Faculty of medicine, Kyoto University) helped us with HTML codes for our wiki.

■Human Practice support

  • Kyoto city
  • Kyoto city forestry cooperative（Ms. Takubo ）
  • Kyoto-Osaka forestry cooperative (Mr. Onishi, Mr. Enotani)

■Thanks and acknowledgements for all other people involved in helping make a successful iGEM team

  • Prof. Keiko Kuroda (Department of Agriculture, Kobe University) provided us with a background on the matter of the pine disease in Japan.
  • Dr. Taisei Kikuchi (Department of Medicine, Miyazaki University) suggested the possibility of culturing B. xylophilus with S. cerevisiae.
  • Mr. Shuhei Yamada (Undergraduate school of Engineering, Kyoto University) helped us with revising our English in wiki and presentation.
  • Dr. Makoto Kashima (Department of Agriculture, Ryukoku University) helped us all through our projects.
  • Mr. Fumitaka Hashiya (Graduate School of Science, Kyoto University) helped us with the registration and storage of reagents.
  • Mr. Tomoki Uchino (Undergraduate School of Agriculture, Kyoto University) trained us on the cloning experiment.