Difference between revisions of "Team:Aix-Marseille/Parts"

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{{Aix-Marseille|title=Parts|toc=__TOC__}}
 
{{Aix-Marseille|title=Parts|toc=__TOC__}}
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<groupparts>iGEM17 Aix-Marseille</groupparts>
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During the summer, several basic parts were constructed.
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Some of these were not designed using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard but, rather, with the [http://parts.igem.org/Assembly_standard_25 Rfc25] in order to construct fusion proteins.
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'''We designed 3 parts for the quorum sensing approach:'''
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*Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
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*Acyl-CoA isomerase  (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
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*Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]
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'''We designed 2 parts to disrupt biofilms:'''
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*Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]
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*EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]
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'''Finally, we designed some basic parts for the phage-like particle approach:'''
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*''Xylella fastidiosa'' constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
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*Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
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*Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]
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Then a [[Team:Aix-Marseille/Part Collection|collection of parts]] to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a [[Team:Aix-Marseille/Part Collection|collection of targeting parts]]:
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*p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
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*p3_N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
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*p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
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*p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
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*p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
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*p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
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*p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
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*p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
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*p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
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*p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
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*p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]

Latest revision as of 02:48, 2 November 2017

Parts

During the summer, several basic parts were constructed. Some of these were not designed using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard but, rather, with the [http://parts.igem.org/Assembly_standard_25 Rfc25] in order to construct fusion proteins.

We designed 3 parts for the quorum sensing approach:

  • Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
  • Acyl-CoA isomerase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
  • Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]

We designed 2 parts to disrupt biofilms:

  • Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]
  • EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]

Finally, we designed some basic parts for the phage-like particle approach:

  • Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
  • Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
  • Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]

Then a collection of parts to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a collection of targeting parts:

  • p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
  • p3_N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
  • p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
  • p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
  • p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
  • p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
  • p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
  • p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
  • p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
  • p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
  • p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]