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{{Aix-Marseille|title=Parts|toc=__TOC__}} | {{Aix-Marseille|title=Parts|toc=__TOC__}} | ||
− | + | During the summer, several basic parts were constructed. | |
+ | Some of these were not designed using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard but, rather, with the [http://parts.igem.org/Assembly_standard_25 Rfc25] in order to construct fusion proteins. | ||
− | + | '''We designed 3 parts for the quorum sensing approach:''' | |
− | + | ||
− | '''We | + | |
− | * | + | *Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000] |
− | * | + | *Acyl-CoA isomerase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001] |
+ | *Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002] | ||
− | '''We | + | '''We designed 2 parts to disrupt biofilms:''' |
− | * | + | *Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003] |
− | * | + | *EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006] |
− | + | ||
− | ''' | + | '''Finally, we designed some basic parts for the phage-like particle approach:''' |
− | * | + | *''Xylella fastidiosa'' constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004] |
+ | *Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005] | ||
+ | *Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007] | ||
− | + | Then a [[Team:Aix-Marseille/Part Collection|collection of parts]] to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a [[Team:Aix-Marseille/Part Collection|collection of targeting parts]]: | |
− | + | *p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008] | |
− | + | *p3_N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009] | |
− | + | *p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010] | |
− | + | *p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011] | |
− | + | *p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012] | |
− | *p3_E.coli (Rfc25) - BBa_K2255008 | + | *p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013] |
− | * | + | *p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014] |
− | *p3_P.aeruginosa (Rfc25) - BBa_K2255010 | + | *p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015] |
− | *p3_R.solanacearum_RSM1 (Rfc25) - BBa_K2255011 | + | *p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016] |
− | *p3_R.solanacearum_RSS1 (Rfc25) - BBa_K2255012 | + | *p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017] |
− | *p3_V.Cholerae_CTXΦ (Rfc25) - BBa_K2255013 | + | *p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018] |
− | *p3_V.Cholerae_fs2 (Rfc25) - BBa_K2255014 | + | |
− | *p3_V.Cholerea_VGJΦ (Rfc25) - BBa_K2255015 | + | |
− | *p3_X.campestris (Rfc25) - BBa_K2255016 | + | |
− | *p3_X.fastidiosa (Rfc25) - BBa_K2255017 | + | |
− | *p3_X.fuscans (Rfc25) - BBa_K2255018 | + |
Latest revision as of 02:48, 2 November 2017
Parts
During the summer, several basic parts were constructed. Some of these were not designed using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard but, rather, with the [http://parts.igem.org/Assembly_standard_25 Rfc25] in order to construct fusion proteins.
We designed 3 parts for the quorum sensing approach:
- Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
- Acyl-CoA isomerase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
- Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]
We designed 2 parts to disrupt biofilms:
- Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]
- EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]
Finally, we designed some basic parts for the phage-like particle approach:
- Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
- Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
- Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]
Then a collection of parts to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a collection of targeting parts:
- p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
- p3_N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
- p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
- p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
- p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
- p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
- p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
- p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
- p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
- p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
- p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]