Difference between revisions of "Team:Aix-Marseille/Parts"

 
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{{Aix-Marseille|title=Parts}}
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{{Aix-Marseille|title=Parts|toc=__TOC__}}
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During the summer, several basic parts were constructed.
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Some of these were not designed using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard but, rather, with the [http://parts.igem.org/Assembly_standard_25 Rfc25] in order to construct fusion proteins.
  
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'''We designed 3 parts for the quorum sensing approach:'''
  
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*Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
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*Acyl-CoA isomerase  (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
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*Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]
  
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'''We designed 2 parts to disrupt biofilms:'''
  
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*Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]
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*EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]
  
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<div class="column full_size">
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'''Finally, we designed some basic parts for the phage-like particle approach:'''
  
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<h1>Parts</h1>
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*''Xylella fastidiosa'' constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
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*Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
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*Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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Then a [[Team:Aix-Marseille/Part Collection|collection of parts]] to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a [[Team:Aix-Marseille/Part Collection|collection of targeting parts]]:
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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*p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
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*p3_N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
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*p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
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*p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
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*p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
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*p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
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*p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
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*p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
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<div class="highlight">
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*p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
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<h5>Note</h5>
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*p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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*p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]
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<div class="column half_size">
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 Aix-Marseille</groupparts>
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Latest revision as of 02:48, 2 November 2017

Parts

During the summer, several basic parts were constructed. Some of these were not designed using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard but, rather, with the [http://parts.igem.org/Assembly_standard_25 Rfc25] in order to construct fusion proteins.

We designed 3 parts for the quorum sensing approach:

  • Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
  • Acyl-CoA isomerase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
  • Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]

We designed 2 parts to disrupt biofilms:

  • Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]
  • EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]

Finally, we designed some basic parts for the phage-like particle approach:

  • Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
  • Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
  • Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]

Then a collection of parts to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a collection of targeting parts:

  • p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
  • p3_N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
  • p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
  • p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
  • p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
  • p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
  • p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
  • p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
  • p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
  • p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
  • p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]