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| <h4>Module 3- Activates in presence of salicylate</h4> | | <h4>Module 3- Activates in presence of salicylate</h4> |
| </section><br /> | | </section><br /> |
− | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/9/99/T--IISER-Mohali-INDIA--circuit2.png" alt="Figure1. Genetic circuit for detection of salicylate" width="100%" height="100%"></td></tr></table></center> | + | <center><div style="img-data"><img src="https://static.igem.org/mediawiki/2017/9/99/T--IISER-Mohali-INDIA--circuit2.png" alt="Figure1. Genetic circuit for detection of salicylate" width="100%" height="100%"></div></center> |
| <br/> | | <br/> |
| <section><h3>To clone three modules in the <i>E.coli</i>, they should have different copy number plasmids. The module 1 will be active even in the absence of pollutant. So, we decided to put it on low copy number plasmid. The module 2 will get activated only after pollutant is present. So, we decided to put it on high copy number plasmid for immediate detection. The module 3 is already present in the <i>E.coli</i> chromosome.</h3><br/> | | <section><h3>To clone three modules in the <i>E.coli</i>, they should have different copy number plasmids. The module 1 will be active even in the absence of pollutant. So, we decided to put it on low copy number plasmid. The module 2 will get activated only after pollutant is present. So, we decided to put it on high copy number plasmid for immediate detection. The module 3 is already present in the <i>E.coli</i> chromosome.</h3><br/> |