Latest revision as of 03:00, 2 November 2017

Urban Tundra | Intelligent Innovation

Protocols

Streaking/Plating Microbial Cultures on Agar

Inoculating Cultures in Liquid Media

Lysogeny Broth Preparation

Lysogeny Agar Plate Preparation

Chemical Transformations

PCR

Restriction Digestion

Ligation of PCR Product into Plasmid

DNA Electrophoresis

InterLab Study (from iGEM website)

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              <h1>Protocols</h1>
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-              <a href="#">HOME > RESEARCH > SAFETY > PROTOCOLS</a>
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-             <h3>Steaking/Plating Microbial Cultures on Agar</h3>
+             <h3><a href="https://static.igem.org/mediawiki/2017/0/0b/Protocol_1.pdf">Streaking/Plating Microbial Cultures on Agar</a></h3>
-<p><b>Goal:</b> To isolate and grow isolated cultures of <i>E. coli</i> on Agar plates<p>
+            <h3><a href="https://static.igem.org/mediawiki/2017/1/14/Protocol_9.pdf">Inoculating Cultures in Liquid Media</a></h3>
-<p><b>Materials:</b><br>Bunsen burner<br>Disposable loops used to streak plates Lysogeny broth(LB) agar plates (with antibiotics if required)<br>Culture of E. coli (in plate or in shake flask/test tube)</p>
+            <h3><a href="https://static.igem.org/mediawiki/2017/2/21/Protocol_2.pdf">Lysogeny Broth Preparation</a></h3>
-<p><b>Procedure:</b><br>1. Ignite the Bunsen burner to maintain a sterile environment<br>2. If culture is in a petri dish: Use the tip of a disposable loop and pick up an isolated culture<br>3. If culture is in a shake flask/test tube: Dip the circular end of the loop into the liquid medium culture<br>4. Close the petri dish or shake flask/test tube<br>5. Transfer the collected sample to the LB agar plates by gently touching the tip/loop end with bacteria on the agar and brush over the plate. Make sure to streak at an angle<br>6. Two streaking methods are possible: Brushing the loop all over the plate once without crossing previous streaks, or brushing the loop over three different thirds of the plate<br>7. Allow streaks to dry before labelling each plate and incubating them at the appropriate temperature</p>
+            <h3><a href="https://static.igem.org/mediawiki/2017/b/b6/Protocol_3.pdf">Lysogeny Agar Plate Preparation</a></h3>
-           <h3>Inoculating Cultures in Liquid Media</h3>
+            <h3><a href="https://static.igem.org/mediawiki/2017/d/d9/Protocol_4.pdf">Chemical Transformations</a></h3>
-<p><b>Goal:</b> To isolate and grow individual colonies of <i>E. coli</i> in a nutrient-rich media<p>
+            <h3><a href="https://static.igem.org/mediawiki/2017/8/88/Protocol_5.pdf">PCR</a></h3>
-<p><b>Materials:</b><br>Test tubes/Shake flask<br>Media (Lysogeny Broth)<br>Petri dishes containing E. coli culture<br>Pipettes<br>Antibiotics (if required)<br>Bunsen burner</p>
+            <h3><a href="https://static.igem.org/mediawiki/2017/7/7d/Protocol_6.pdf">Restriction Digestion</a></h3>
-<p><b>Procedure:</b><br>1. Ignite the Bunsen burner to maintain a sterile environment<br>2. Obtain a shake flask/test tube and transfer the desired amount of media into the flask/tube. If required, add the appropriate amount of antibiotics<br>3. Open the petri dishes containing the E. coli cultures. Use a pipette tip and carefully pick a single colony from the plate<br>4. Transfer the colony on the pipette tip to the media in the flask/tube<br>5. Label the flask/tube and incubate in a shaker at an appropriate temperature</p>
+            <h3><a href="https://static.igem.org/mediawiki/2017/9/97/Protocol_7.pdf">Ligation of PCR Product into Plasmid</a></h3>
+            <h3><a href="https://static.igem.org/mediawiki/2017/f/fc/Protocol_8.pdf">DNA Electrophoresis</a></h3>
+            <h3><a href="https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf">InterLab Study (from iGEM website)</a></h3>
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Difference between revisions of "Team:UrbanTundra Edmonton/Protocols"

Latest revision as of 03:00, 2 November 2017

Protocols