Difference between revisions of "Team:BIT/nb"

 
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<h2>Market Analysis</h2>
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<h2>Journal</h2>
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    <p>&nbsp;</p>
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<p><span class="label-warning"><font size=4><font color=black> March: </font> </font></span></p>
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   </span><p>&nbsp;&nbsp;<br>
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  <p>&nbsp;</p>
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i. Week 1-2 (2017.03.04-2017.03.17)
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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<p>&nbsp;</p>
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<p><span class="label-warning">a. Four Aspects:</span><br>
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  <span class="lead">&nbsp;&nbsp;
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  i. Strength:
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   </span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; 1. Convenience.
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   <br>
 
   <br>
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   &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2. Adaptability.
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   &nbsp;&nbsp;&nbsp;  
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  <br>
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After discussion, the main goal of our project was to detect cancer of the liver by using small molecule
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; 3. High Cost/Performance Ratio.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;
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  4. Inelastic to change in test conditions.<br>
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  <span class="lead">&nbsp;&nbsp;
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  ii. Weakness:</span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  1. Technology Barrier.
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<br>
 
<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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ii. Week 3 (2017.03.18-2017.03.24)
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  2. Branding Barrier.
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<br>
 
<br>
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   &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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   &nbsp;&nbsp;&nbsp;  
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  3. Strict Market Admittance Criteria.<br>
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After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria.
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  4. Distribution Barrier.<br>
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  <span class="lead">&nbsp;&nbsp;
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  iii. Opportunity:</span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  1. Favorable national economic condition.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  2. Positive national policies.<br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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  3. Uprising Health-consciousness.<br>
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  <span class="lead">&nbsp;&nbsp;
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  iv. Threats:</span><br>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;
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  1. Well-established industries control the high-end markets with related products.
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<br>
 
<br>
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   &nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
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  2. Industrial chain is currently complete
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iii. week 4 (2017.03.25-2017.03.31)</span><br>
 +
   &nbsp;&nbsp;&nbsp;
 +
We chose our main experimental method.<br>
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 +
</p>
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 +
 
 +
      <p>&nbsp;</p>
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<p><span class="label-warning"><font size=4><font color=black> April: </font> </font></span></p>
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  </span><p>&nbsp;&nbsp;<br>
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i. Week 5 (2017.04.01-2017.04.07)
 
<br>
 
<br>
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   &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;
+
   &nbsp;&nbsp;&nbsp;  
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  3. Technological applications are matured</p>
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We listed the plasmids we might use, designed the gene lines, began to construct our part.
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  <p> <span class="label-warning">b. Comments: </span><br>
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−
&nbsp;&nbsp; Comparing to technology equipped by clinics and hospitals, The use of adjustable aptamer and genetic modified microbes made JACOB more economical, adaptable, and stable. The integration of automated proceeding made JACOB more user-friendly and portable. JACOB does not need professional physicians reading results, behaving like glucose meter but less risky. In China it is likely to be deployed in units of household or local communities. Similarly, for United States the Food and Drug Administration classified the core mechanism of JACOB as Class I medical device (if designed to not detecting cancer biomarkers), means no premarketing control is required. It is worth noting though, Chinese in vitro diagnostic market is relatively new and have few competitors. Those few players can no longer support the concrete increasing demand for medical cares. Chinese government had ratified a great number of acts as well as guidelines and simplified documents examining and approval process to encourage innovation in the field of medical devices, which offers our team a great market environment. </p>
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<br>
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<div id="fh5co-wireframe">
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ii. Week 6 2017.04.08-2017.04.14)
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</div>
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<br>
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</div>
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  &nbsp;&nbsp;&nbsp;  
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</div>
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We constructed our first part BBa_K2305008.
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<div class="第一段">
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<div class="row animate-box">
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<div class="col-md-8 col-md-offset-2 text-center fh5co-heading">
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<h2>Wireframe Connects the Underlying Conceptual Structure</h2><p>In market analysis we had solved many issues regarding our project background, but also generated more questions about operability. To obtain a better answer, we had visited 北京永翰星港生物技术有限公司和博奥生物芯片公司. </p>
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<h3></h3>
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<p>We asked 蓝晶 mainly on how to construct a viable procedure to industrialize JACOB. Our plan for JACOB after the iGEM jamboree is divided into developments, commercialization, and resource transformation. For development we currently divided it into small, medium, large scale trial. Based on our current knowledge, small and medium trial is planned as below  </p>
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−
<span>Louie Jie Mahusay</span><br>
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−
<small>CEO, Founder</small>
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</div>
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</div>
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<div class="col-md-7 animate-box">
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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<blockquote>
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<p>Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts far from the countries Vokalia and Consonantia, there live the blind texts. </p>
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</blockquote>
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−
<p><span class="label-warning">1. Small Scale Trial</span><br>
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−
                    &nbsp;&nbsp;&nbsp; a. Duration: About half year
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−
                      <br>
+
−
                    &nbsp;&nbsp;&nbsp; b. We will choose the production method for JACOB, e.g. as we learned from 永翰星港, the "filtration" of aptamers are still expensive, but aptamers synthesis is more economical. Nine sets of known aptamer (90np, 15 microliter, 100nmol/L) costs only about $135, and every set is sufficient for 250 microfluidic chips.<br>
+
−
                    &nbsp;&nbsp;&nbsp;
+
−
c. During this phase we also need to rule out unknown influences on the device caused by switching to new assembly conditions, such as switching to fermentation flask instead of tightly controlled experiment chamber.  
+
 
<br>
 
<br>
−
&nbsp;&nbsp;&nbsp;
 
−
d. During this phase preserving microbes will greatly affect the cost of production, and affordability is one of the most important characteristics of JACOB.<br>
 
−
<span class="label-warning">2. Medium Scale Trial</span><br>
 
−
&nbsp;&nbsp;&nbsp;
 
−
a. Duration: 4-5 months<br>
 
−
&nbsp;&nbsp;&nbsp;
 
−
b. When the production of microfluidic chip is resolved, how to prepare it for using will be on the agenda. Currently the chip we used for final packaging needs to be soaked in ethyl alcohol and acetone for about one hour, which pose a question for mass production. </p>
 
−
</div>
 
−
<p>We asked 博奥 mainly on how to control the cost of microfluidic chip production¬. From their experience we found that chips cost much more in the early development, but its cost diminishes quickly in later stages when mass production is reached, ranging from $0.1 to few dollars. However, there exist a conflict of material. In the iGEM version we picked optical cement instead of PMDS for its advantages of adequate solidification speed, feasibility, bio-inertia, and precision of altitude. However, PMDS production industries are much more matured. There is currently no companies offering mass production of optical cement microfluidic chips. Thus, we must decide the better material.</p>
 
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</div>
 
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</div>
 
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</div>
 
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</div>
 
  
 +
iii. Week 7 (2017.04.15-2017.04.21)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.<br>
 +
 
 +
iv.    Week 8 (2017.04.22-2017.04.28)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.<br>
 +
</p>
  
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<h2>Hire Us!</h2>
 
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<p>Facilis ipsum reprehenderit nemo molestias. Aut cum mollitia reprehenderit. Eos cumque dicta adipisci architecto culpa amet.</p>
 
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<p><a href="#" class="btn btn-default btn-lg">Contact Us</a></p>
 
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      <p>&nbsp;</p>
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<p><span class="label-warning"><font size=4><font color=black>May: </font> </font></span></p>
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<div class="row row-pb-md">
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  </span><p>&nbsp;&nbsp;<br>
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<div class="col-md-4 fh5co-widget">
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i. Week 9 (2017.04.29-2017.05.05)
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<h4>Air</h4>
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<br>
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<p>Facilis ipsum reprehenderit nemo molestias. Aut cum mollitia reprehenderit. Eos cumque dicta adipisci architecto culpa amet.</p>
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  &nbsp;&nbsp;&nbsp;
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</div>
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We constructed BBa_K2305009、BBa_K2305010.
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<div class="col-md-2 col-md-push-1 fh5co-widget">
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<h4>Links</h4>
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<ul class="fh5co-footer-links">
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<li><a href="home.html">Home</a></li>
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<li><a href="#">Team</a></li>
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<li><a href="Achievement.html">Achievement</a></li>
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<li><a href="notebook.html">Notebook</a></li>
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</ul>
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</div>
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<div class="col-md-2 col-md-push-1 fh5co-widget">
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<br>
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<h4>Categories</h4>
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ii. Week 10 (2017.05.06-2017.05.12)
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<ul class="fh5co-footer-links">
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<br>
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<li><a href="#">Landing Page</a></li>
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  &nbsp;&nbsp;&nbsp;
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<li><a href="#">Real Estate</a></li>
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We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016.
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<li><a href="#">Personal</a></li>
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<br>
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<li><a href="#">Business</a></li>
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<li><a href="#">e-Commerce</a></li>
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</ul>
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</div>
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<div class="col-md-4 col-md-push-1 fh5co-widget">
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iii. Week 11 (2017.05.13-2017.05.19)
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<h4>Contact Information</h4>
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<br>
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<ul class="fh5co-footer-links">
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  &nbsp;&nbsp;&nbsp;
−
<li>198 West 21th Street, <br> Suite 721 New York NY 10016</li>
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We constructed BBa_K2305007、BBa_K2305006.<br>
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<li><a href="tel://1234567920">+ 1235 2355 98</a></li>
+
 
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<li><a href="mailto:info@yoursite.com">info@yoursite.com</a></li>
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iv.    Week 12 (2017.05.20-2017.05.26)
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<li><a href="http://#">freehtml5.co</a></li>
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<br>
−
</ul>
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  &nbsp;&nbsp;&nbsp;
−
</div>
+
We constructed BBa_K2305016、BBa_K2305005.<br>
 +
v.     Week 13 (2017.05.27-2017.06.02)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed BBa_K2305004、BBa_K2305013.<br>
 +
</p>
 +
  
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</div>
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      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>June: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 14 (2017.06.03-2017.06.09)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids.
  
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<div class="row copyright"> </div>
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<br>
 +
ii. Week 15-17 (2017.06.10-2017.06.30)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids.
 +
<br>
 +
</p>
  
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</div>
 
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</footer>
 
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</div>
 
  
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<div class="gototop js-top">
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      <p>&nbsp;</p>
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<a href="#" class="js-gotop"><i class="icon-arrow-up22"></i></a>
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<p><span class="label-warning"><font size=4><font color=black>July: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 18 (2017.07.01-2017.07.07)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We tried to knock out the lysa gene in E.coli by λRed recombination system.
 +
 
 +
<br>
 +
ii. Week 19 ( 2017.07.08-2017.07.14)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We discussed about the problems we met in the gene-knocked.
 +
<br>
 +
 
 +
iii. Week 20-21 (2017.07.15-2017.07.28)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We learned how to produce competent cells and produced enough cells..<br>
 +
</p>
 +
 
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>August: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 22-23 (2017.07.29-2017.08.16)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We import some plasmids we constructed to lysine-deficinent E.coli.
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 +
<br>
 +
ii. Week 24-25 (  2017.08.12-2017.08.25)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids.
 +
<br>
 +
 
 +
iii. Week 26 (2017.08.26-2017.09.01)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
To achieve the standard of silver medal, we sorted out 19 parts completed in totally.<br>
 +
</p>
 +
 
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black> September: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 27-28 (2017.09.02-2017.09.15)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032)
 +
 
 +
<br>
 +
ii. Week 29-30 (  2017.09.16-2017.09.29)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070
 +
<br></p>
 +
 
 +
 
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>October: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 31-32 (2017.09.30-2017.10.13)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We sorted out our results and steps of the experiment.
 +
 
 +
<br>
 +
ii. Week 33-34 (2017.10.14-2017.10.31)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki.
 +
<br>
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</p>
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</div>
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          <h2>Contact Us!</h2>
 +
          <p><center>Please keep reading if you want to contact us.</center></p>
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Latest revision as of 03:04, 2 November 2017

Journal

 

March:

  
i. Week 1-2 (2017.03.04-2017.03.17)
    After discussion, the main goal of our project was to detect cancer of the liver by using small molecule
ii. Week 3 (2017.03.18-2017.03.24)
    After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria.
iii. week 4 (2017.03.25-2017.03.31)
    We chose our main experimental method.

 

April:

  
i. Week 5 (2017.04.01-2017.04.07)
    We listed the plasmids we might use, designed the gene lines, began to construct our part.
ii. Week 6 2017.04.08-2017.04.14)
    We constructed our first part BBa_K2305008.
iii. Week 7 (2017.04.15-2017.04.21)
    We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.
iv. Week 8 (2017.04.22-2017.04.28)
    We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.

 

May:

  
i. Week 9 (2017.04.29-2017.05.05)
    We constructed BBa_K2305009、BBa_K2305010.
ii. Week 10 (2017.05.06-2017.05.12)
    We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016.
iii. Week 11 (2017.05.13-2017.05.19)
    We constructed BBa_K2305007、BBa_K2305006.
iv. Week 12 (2017.05.20-2017.05.26)
    We constructed BBa_K2305016、BBa_K2305005.
v. Week 13 (2017.05.27-2017.06.02)
    We constructed BBa_K2305004、BBa_K2305013.

 

June:

  
i. Week 14 (2017.06.03-2017.06.09)
    We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids.
ii. Week 15-17 (2017.06.10-2017.06.30)
    We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids.

 

July:

  
i. Week 18 (2017.07.01-2017.07.07)
    We tried to knock out the lysa gene in E.coli by λRed recombination system.
ii. Week 19 ( 2017.07.08-2017.07.14)
    We discussed about the problems we met in the gene-knocked.
iii. Week 20-21 (2017.07.15-2017.07.28)
    We learned how to produce competent cells and produced enough cells..

 

August:

  
i. Week 22-23 (2017.07.29-2017.08.16)
    We import some plasmids we constructed to lysine-deficinent E.coli.
ii. Week 24-25 ( 2017.08.12-2017.08.25)
    We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids.
iii. Week 26 (2017.08.26-2017.09.01)
    To achieve the standard of silver medal, we sorted out 19 parts completed in totally.

 

September:

  
i. Week 27-28 (2017.09.02-2017.09.15)
    To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032)
ii. Week 29-30 ( 2017.09.16-2017.09.29)
    To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070

 

October:

  
i. Week 31-32 (2017.09.30-2017.10.13)
    We sorted out our results and steps of the experiment.
ii. Week 33-34 (2017.10.14-2017.10.31)
    We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki.

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