Difference between revisions of "Team:BIT/nb"
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<div class="col-xs-10 text-right menu-1"> | <div class="col-xs-10 text-right menu-1"> | ||
<ul> | <ul> | ||
| − | <li class=" | + | |
| − | + | ||
| + | <li class="has-dropdown"> | ||
| + | <a>Project</a> | ||
| + | <ul class="dropdown"> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Design">Design</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Demonstrate">Demonstrate</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Model">Model</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
<li class="has-dropdown"> | <li class="has-dropdown"> | ||
| − | <a href="../ | + | <a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a> |
<ul class="dropdown"> | <ul class="dropdown"> | ||
| − | <li><a href=" | + | <li><a href="https://2017.igem.org/Team:BIT/Medal">Medal</a></li> |
| − | <li><a href=" | + | <li><a href="https://2017.igem.org/Team:BIT/Prize">Prize</a></li> |
| − | <li><a href=" | + | <li><a href="https://2017.igem.org/Team:BIT/Parts">Part</a></li> |
| + | <li><a href="https://2017.igem.org/Team:BIT/Safety">Safety Form</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="../ | + | |
| − | <li><a href="../ | + | <li class="has-dropdown"> |
| − | <li class="btn-cta"><a href=" | + | <a href="https://2017.igem.org/Team:BIT/HP">Practice</a> |
| + | <ul class="dropdown"> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/HP/Silver">Silver</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/HP/Gold_Integrated">Gold</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Engagement"> Engagement</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Entrepreneurship">Entreperneurship</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="has-dropdown"> | ||
| + | <a href="https://2017.igem.org/Team:BIT/Notebook"style="color:#1b8fac">Notebook</a> | ||
| + | <ul class="dropdown"> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Gallery">Gallery</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Track">Track</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/nb">Journal</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Protocol">Protocol</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="has-dropdown"> | ||
| + | <a href="https://2017.igem.org/Team:BIT/Team">Team</a> | ||
| + | <ul class="dropdown"> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Collaborations">Collaborations</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Team/Member">Member</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Attributions">Attributions</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="btn-cta"><a href="https://2017.igem.org/Team:BIT"><span>Home</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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</div> | </div> | ||
</nav> | </nav> | ||
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<p> </p> | <p> </p> | ||
<p><span class="label-warning"><font size=4><font color=black> March: </font> </font></span></p> | <p><span class="label-warning"><font size=4><font color=black> March: </font> </font></span></p> | ||
| − | </span><p> | + | </span><p> <br> |
| − | + | i. Week 1-2 (2017.03.04-2017.03.17) | |
| − | i. | + | |
<br> | <br> | ||
| − | After discussion, the main goal of our project was to detect cancer of the liver by using small molecule | + | |
| + | After discussion, the main goal of our project was to detect cancer of the liver by using small molecule | ||
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<br> | <br> | ||
| − | + | ii. Week 3 (2017.03.18-2017.03.24) | |
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<br> | <br> | ||
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| − | + | After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria. | |
<br> | <br> | ||
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| + | iii. week 4 (2017.03.25-2017.03.31)</span><br> | ||
| + | | ||
| + | We chose our main experimental method.<br> | ||
| + | |||
| + | </p> | ||
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| + | <p> </p> | ||
<p><span class="label-warning"><font size=4><font color=black> April: </font> </font></span></p> | <p><span class="label-warning"><font size=4><font color=black> April: </font> </font></span></p> | ||
| − | <span | + | </span><p> <br> |
| − | <p> | + | i. Week 5 (2017.04.01-2017.04.07) |
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<br> | <br> | ||
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| − | + | We listed the plasmids we might use, designed the gene lines, began to construct our part. | |
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<br> | <br> | ||
| − | + | ii. Week 6 2017.04.08-2017.04.14) | |
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| − | + | We constructed our first part BBa_K2305008. | |
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| − | + | iii. Week 7 (2017.04.15-2017.04.21) | |
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<br> | <br> | ||
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| − | + | We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.<br> | |
| + | |||
| + | iv. Week 8 (2017.04.22-2017.04.28) | ||
<br> | <br> | ||
| − | + | | |
| − | + | We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.<br> | |
| − | + | </p> | |
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| − | + | ||
| − | + | <p> </p> | |
| − | + | <p><span class="label-warning"><font size=4><font color=black>May: </font> </font></span></p> | |
| − | + | </span><p> <br> | |
| − | + | i. Week 9 (2017.04.29-2017.05.05) | |
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<br> | <br> | ||
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| − | + | We constructed BBa_K2305009、BBa_K2305010. | |
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<br> | <br> | ||
| − | + | ii. Week 10 (2017.05.06-2017.05.12) | |
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<br> | <br> | ||
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| − | + | We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016. | |
<br> | <br> | ||
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| − | + | iii. Week 11 (2017.05.13-2017.05.19) | |
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<br> | <br> | ||
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| − | + | We constructed BBa_K2305007、BBa_K2305006.<br> | |
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| + | iv. Week 12 (2017.05.20-2017.05.26) | ||
<br> | <br> | ||
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| − | + | We constructed BBa_K2305016、BBa_K2305005.<br> | |
| − | + | v. Week 13 (2017.05.27-2017.06.02) | |
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<br> | <br> | ||
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| − | + | We constructed BBa_K2305004、BBa_K2305013.<br> | |
| + | </p> | ||
| + | |||
| + | |||
| + | <p> </p> | ||
| + | <p><span class="label-warning"><font size=4><font color=black>June: </font> </font></span></p> | ||
| + | </span><p> <br> | ||
| + | i. Week 14 (2017.06.03-2017.06.09) | ||
<br> | <br> | ||
| − | + | | |
| − | + | We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids. | |
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| − | + | ii. Week 15-17 (2017.06.10-2017.06.30) | |
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<br> | <br> | ||
| − | + | | |
| − | + | We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids. | |
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| − | + | </p> | |
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| + | <p> </p> | ||
| + | <p><span class="label-warning"><font size=4><font color=black>July: </font> </font></span></p> | ||
| + | </span><p> <br> | ||
| + | i. Week 18 (2017.07.01-2017.07.07) | ||
<br> | <br> | ||
| − | + | | |
| − | + | We tried to knock out the lysa gene in E.coli by λRed recombination system. | |
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| − | + | ii. Week 19 ( 2017.07.08-2017.07.14) | |
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<br> | <br> | ||
| − | + | | |
| − | + | We discussed about the problems we met in the gene-knocked. | |
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| − | + | iii. Week 20-21 (2017.07.15-2017.07.28) | |
<br> | <br> | ||
| − | + | | |
| − | + | We learned how to produce competent cells and produced enough cells..<br> | |
| − | + | </p> | |
| − | + | ||
| − | + | <p> </p> | |
| − | + | <p><span class="label-warning"><font size=4><font color=black>August: </font> </font></span></p> | |
| − | + | </span><p> <br> | |
| − | + | i. Week 22-23 (2017.07.29-2017.08.16) | |
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<br> | <br> | ||
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| − | + | We import some plasmids we constructed to lysine-deficinent E.coli. | |
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<br> | <br> | ||
| − | + | ii. Week 24-25 ( 2017.08.12-2017.08.25) | |
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<br> | <br> | ||
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| − | + | We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids. | |
<br> | <br> | ||
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| − | + | iii. Week 26 (2017.08.26-2017.09.01) | |
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<br> | <br> | ||
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| − | + | To achieve the standard of silver medal, we sorted out 19 parts completed in totally.<br> | |
| + | </p> | ||
| + | |||
| + | <p> </p> | ||
| + | <p><span class="label-warning"><font size=4><font color=black> September: </font> </font></span></p> | ||
| + | </span><p> <br> | ||
| + | i. Week 27-28 (2017.09.02-2017.09.15) | ||
<br> | <br> | ||
| − | + | | |
| − | + | To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032) | |
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| + | <br> | ||
| + | ii. Week 29-30 ( 2017.09.16-2017.09.29) | ||
| + | <br> | ||
| + | | ||
| + | To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070 | ||
| + | <br></p> | ||
| − | < | + | <p> </p> |
| − | + | <p><span class="label-warning"><font size=4><font color=black>October: </font> </font></span></p> | |
| − | + | </span><p> <br> | |
| − | + | i. Week 31-32 (2017.09.30-2017.10.13) | |
| − | + | <br> | |
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| − | + | We sorted out our results and steps of the experiment. | |
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| − | + | <br> | |
| − | + | ii. Week 33-34 (2017.10.14-2017.10.31) | |
| − | + | <br> | |
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| − | + | We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki. | |
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| − | + | </p> | |
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| + | </div> | ||
</div> | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| − | |||
| − | + | ||
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| + | <div class="row animate-box"> | ||
| + | <div class="col-md-8 col-md-offset-2 text-center fh5co-heading"> | ||
| + | <h2>Contact Us!</h2> | ||
| + | <p><center>Please keep reading if you want to contact us.</center></p> | ||
| + | |||
| + | </div> | ||
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| + | <h4>BIT</h4> | ||
| + | <p>It's an excellent Team with thoughtful persons full of funs to the academic</p> | ||
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| + | <h4>Links</h4> | ||
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| + | <li><a href="https://2017.igem.org/Team:BIT">Home</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Team">Team</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:BIT/Notebook">Notebook</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-md-4 col-md-push-1 fh5co-widget"> | ||
| + | <h4>Contact Information</h4> | ||
| + | <ul class="fh5co-footer-links"> | ||
| + | <li><a href="">Beijing Institute of Technology No.5 Yard,Zhong Guan Cun South Street Haidian District, Beijing, CHINA</li> | ||
| + | <li><a href="">Wechat:iGEM_BIT</a></li> | ||
| + | <li><a href="">igem_bit@outlook.com</a></li> | ||
| + | <li><a href="">Facebook:iGEM_BIT</li> | ||
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Latest revision as of 03:04, 2 November 2017
Journal
March:
i. Week 1-2 (2017.03.04-2017.03.17)
After discussion, the main goal of our project was to detect cancer of the liver by using small molecule
ii. Week 3 (2017.03.18-2017.03.24)
After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria.
iii. week 4 (2017.03.25-2017.03.31)
We chose our main experimental method.
April:
i. Week 5 (2017.04.01-2017.04.07)
We listed the plasmids we might use, designed the gene lines, began to construct our part.
ii. Week 6 2017.04.08-2017.04.14)
We constructed our first part BBa_K2305008.
iii. Week 7 (2017.04.15-2017.04.21)
We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.
iv. Week 8 (2017.04.22-2017.04.28)
We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.
May:
i. Week 9 (2017.04.29-2017.05.05)
We constructed BBa_K2305009、BBa_K2305010.
ii. Week 10 (2017.05.06-2017.05.12)
We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016.
iii. Week 11 (2017.05.13-2017.05.19)
We constructed BBa_K2305007、BBa_K2305006.
iv. Week 12 (2017.05.20-2017.05.26)
We constructed BBa_K2305016、BBa_K2305005.
v. Week 13 (2017.05.27-2017.06.02)
We constructed BBa_K2305004、BBa_K2305013.
June:
i. Week 14 (2017.06.03-2017.06.09)
We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids.
ii. Week 15-17 (2017.06.10-2017.06.30)
We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids.
July:
i. Week 18 (2017.07.01-2017.07.07)
We tried to knock out the lysa gene in E.coli by λRed recombination system.
ii. Week 19 ( 2017.07.08-2017.07.14)
We discussed about the problems we met in the gene-knocked.
iii. Week 20-21 (2017.07.15-2017.07.28)
We learned how to produce competent cells and produced enough cells..
August:
i. Week 22-23 (2017.07.29-2017.08.16)
We import some plasmids we constructed to lysine-deficinent E.coli.
ii. Week 24-25 ( 2017.08.12-2017.08.25)
We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids.
iii. Week 26 (2017.08.26-2017.09.01)
To achieve the standard of silver medal, we sorted out 19 parts completed in totally.
September:
i. Week 27-28 (2017.09.02-2017.09.15)
To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032)
ii. Week 29-30 ( 2017.09.16-2017.09.29)
To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070
October:
i. Week 31-32 (2017.09.30-2017.10.13)
We sorted out our results and steps of the experiment.
ii. Week 33-34 (2017.10.14-2017.10.31)
We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki.
