Difference between revisions of "Team:SZU-China/Basic Part"

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{{SZU-China}}
 
 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Basic Parts</h1>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
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<br>
 
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<h3>Best Basic Part Special Prize</h3>
 
  
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:SZU-China/sub/CSS?action=raw&amp;ctype=text/css">
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<h4>Note</h4>
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    <link href="https://2017.igem.org/Template:SZU-China/HP/CSS?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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    <link href="https://2017.igem.org/Template:SZU-China/table/CSS?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
  
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<body>
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    <div class="navbar" style="top:5px;position:fixed;z-index:5">
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        <div class="navbar-nav">
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            <ul id="nav-list-ul" class="nav-list">
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                <li>
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                    <a href="#">TEAM</a>
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                    <span class="caret"></span>
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                    <ul class="sub-nav" style="top:-5px">
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Team">MEMBERS</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Collaborations">COLLABORATIONS</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Attributions">ATTRIBUTION</a></li>
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                    </ul>
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                </li>
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                <li>
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                    <a href="#">PRACTICE</a>
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                    <span class="caret"></span>
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                    <ul class="sub-nav" style="top:-5px">
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                        <li><a href="https://2017.igem.org/Team:SZU-China/HP/Gold_Integrated">INTEGRATED HP</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/HP/Silver">SILVER HP</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Engagement">ENGAGEMENT</a></li>
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                    </ul>
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                </li>
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                <li>
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                    <a href="#">EXPERIMENT</a>
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                    <span class="caret"></span>
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                    <ul class="sub-nav" style="top:-5px">
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Procedure">PROCEDURE</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Results">RESULTS</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Demonstrate">DEMONSTRATE</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Protocol">PROTOCOL</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Notebook">NOTEBOOK</a></li>
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                    </ul>
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                </li>
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                <li>
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                    <a href="#">PROJECT</a>
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                    <span class="caret"></span>
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                    <ul class="sub-nav" style="top:-5px">
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Description">DESCRIPTION</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Design">DESIGN</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Model">MODEL</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Safety">SAFTY</a></li>
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                    </ul>
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                </li>
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                <li>
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                    <a href="#">ACHIEVEMENT</a>
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                    <span class="caret"></span>
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                    <ul class="sub-nav" style="top:-5px">
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Achievement">MEDAL</a></li>
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                        <li><a href="https://2017.igem.org/Team:SZU-China/Parts">PARTS</a></li>
 +
                    </ul>
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                </li>
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            </ul>
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        </div>
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        <div>
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            <a href="https://2017.igem.org/Team:SZU-China"><img src="https://static.igem.org/mediawiki/2017/1/17/T--SZU-China--team-logo.png" style="height: 36px;width: auto;padding-top: 12px;padding-left:10px;"></a>
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        </div>
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    </div>
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 +
    <div style="position: relative;top: 40px;">
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        <section id="overview" style="padding:106px 0 80px 0;background-color:white;">
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            <div class="container">
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                <div class="row">
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                    <div class="col-sm-12">
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                        <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:50px"><center>PARTS</center> </h3>
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                    </div>
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                </div>
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            </div>
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        </section>
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        <p></p><br /><br />
 +
        <section id="overview" class="cd-section" style="padding: 66px 0 50px 0; background-color: rgba(245,245,245,0.45);">
 +
            <div style="position: relative;margin: 30px 14%;">
 +
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                <p></p>
 +
                <div class="col-sm-12">
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                    <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Basic Parts</center> </h3>
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                    <span class="font1">This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na<sup>+</sup>-H<sup>+</sup> antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.</span><br /><br />
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                    <br/>
 +
                </div>
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                <p></p>
 +
                <table class="table" style="width:75%;margin:0 auto">
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                    <tbody>
 +
                        <tr>
 +
                            <th style="width:120px">Part Number</th>
 +
                            <th style="width:150px">Name</th>
 +
                            <th style="width:80px">Type</th>
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 +
                            <th style="width:300px">Part Description</th>
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 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232000">BBa_K2232000</a></td>
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                            <td>TSLV1-CA-&#945;</td>
 +
                            <td>Basic</td>
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 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Carbonic Anhydrase α from<i> Bacillus Subtilus TSLV1 </i></td>
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 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232005">BBa_K2232005</a></td>
 +
                            <td>OF4-CA-&#945;</td>
 +
                            <td>Basic</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Carbonic Anhydrase α from<i> Bacillus Subtilus OF4 </i></td>
 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232001">BBa_K2232001</a></td>
 +
                            <td>OF4-nhaC</td>
 +
                            <td>Basic</td>
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 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus OF4 </i></td>
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 +
                        </tr>
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 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232002">BBa_K2232002</a></td>
 +
                            <td>c125-nhaC</td>
 +
                            <td>Basic</td>
 +
 +
                            <td>Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus c125</i></td>
 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232007">BBa_K2232007</a></td>
 +
                            <td>BS168-nhaC</td>
 +
                            <td>Basic</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus 168 </i></td>
 +
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232003">BBa_K2232003</a></td>
 +
                            <td>c125-TupA</td>
 +
                            <td>Basic</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for another Na<sup>+</sup> - H<sup>+</sup> antiporter TupA from<i> Bacillus Subtilus c125 </i></td>
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 +
                        </tr>
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 +
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                    </tbody>
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                </table>
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                <br /><br />
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                <p></p><p></p>
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                <span class="font1"></span>
 +
                <span></span><br />
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                <span></span><br />
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                <span></span><br />
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            </div>
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 +
            <div style="position: relative;margin: 30px 14%;">
 +
 +
                <p></p>
 +
                <div class="col-sm-12">
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                    <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Composite Parts</center> </h3>
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                    <span class="font1">The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.</span><br /><br />
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 +
                </div>
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                <p></p>
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                <br />
 +
                <table class="table" style="width:75%;margin:0 auto">
 +
                    <tbody>
 +
                        <tr>
 +
                            <th style="width:120px">Part Number</th>
 +
                            <th style="width:150px">Name</th>
 +
                            <th style="width:80px">Type</th>
 +
 +
                            <th style="width:300px;padding-left:5px">Part Description</th>
 +
 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232011">BBa_K2232011</a></td>
 +
                            <td>RBS-c125-TupA-T1</td>
 +
                            <td>Composite</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Native sequence from <i>  Mycobacterium NBB4 </i>, contained within the ethylene metabolism operon</td>
 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232012">BBa_K2232012</a></td>
 +
                            <td>P43-OF4-nhaC-T1</td>
 +
                            <td>Composite</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding sequence of EtnR1 from <i>Mycobacterium NBB4 </i>, codon-optimised for expression in <i> E. coli</i> K12</td>
 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232013">BBa_K2232013</a></td>
 +
                            <td>p43-nhaC-T1</td>
 +
                            <td>Composite</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding sequence of EtnR1 from <i>Mycobacterium </i> NBB4, codon-optimised for expression in<i> E. coli </i>K12</td>
 +
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232014">BBa_K2232014</a></td>
 +
                            <td>p43-TSLV1-CA-&#945;</td>
 +
                            <td>Composite</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding region of the pink mutant version of amilCP</td>
 +
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232015">BBa_K2232015</a></td>
 +
                            <td>P43-OF4-CA-&#945;</td>
 +
                            <td>Composite</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding region of the purple mutant version of amilCP</td>
 +
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232026">BBa_K2232026</a></td>
 +
                            <td>p43-CA-β</td>
 +
                            <td>Composite</td>
 +
 +
                            <td style="width:300px;padding-left:5px">Coding region of the green mutant version of amilCP</td>
 +
 +
                        </tr>
 +
                    </tbody>
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                <div class="col-sm-12">
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                    <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Improved Parts</center> </h3>
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                </div>
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                <span class="font1">This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (<span style="color:#808080">in grey</span>) are presented below. You can click on the part number to see the details.</span><br /><br />
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                <p></p><br/>
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                <table class="table" style="width:75%;margin:0 auto">
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                    <tbody>
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                        <tr>
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                            <th style="width:120px;">Part Number</th>
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                            <th style="width:120px">Name</th>
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                            <th style="width:85px">Type</th>
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                            <th style="width:200px">Part Description</th>
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                            <th style="width:150px">Improvement</th>
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                        </tr>
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                        <tr>
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                            <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232020">BBa_K2232020</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K911008" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K911008</a></td>
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                            <td>PsspB-gerAa</td>
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                            <td>Composite</td>
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 +
                            <td style="padding-left:5px">Germination-promoting  receptor (gerA) expression system</td>
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                            <td>Optimize its sequence</td>
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                        </tr>
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                        <tr>
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                            <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232022">BBa_K2232022</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1465205" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K2465205</a></td>
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                            <td>p43-csos3</td>
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                            <td>Composite</td>
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                            <td style="padding-left:5px">Carbonic anhydrase (csoS3) expression system</td>
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                            <td>Optimize its sequence</td>
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                        </tr>
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                        <tr>
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                            <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232025">BBa_K2232025</a><br /><a href="http://parts.igem.org/Part:BBa_K302035" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K302035</a></td>
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                            <td>mazEF</td>
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                            <td>Composite</td>
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                            <td style="padding-left:5px">Coding region of Sucrose-limitation induced kill switch</td>
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                            <td>Characterize in <i>Bacillus Subtilus</i></td>
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                        </tr>
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                    </tbody>
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                </table>
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            </div>
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        </section>
  
 
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Latest revision as of 03:10, 2 November 2017

PARTS



Basic Parts

This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na+-H+ antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.


Part Number Name Type Part Description
BBa_K2232000 TSLV1-CA-α Basic Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus TSLV1
BBa_K2232005 OF4-CA-α Basic Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus OF4
BBa_K2232001 OF4-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus OF4
BBa_K2232002 c125-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus c125
BBa_K2232007 BS168-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus 168
BBa_K2232003 c125-TupA Basic Coding sequence for another Na+ - H+ antiporter TupA from Bacillus Subtilus c125





Composite Parts

The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.


Part Number Name Type Part Description
BBa_K2232011 RBS-c125-TupA-T1 Composite Native sequence from Mycobacterium NBB4 , contained within the ethylene metabolism operon
BBa_K2232012 P43-OF4-nhaC-T1 Composite Coding sequence of EtnR1 from Mycobacterium NBB4 , codon-optimised for expression in E. coli K12
BBa_K2232013 p43-nhaC-T1 Composite Coding sequence of EtnR1 from Mycobacterium NBB4, codon-optimised for expression in E. coli K12
BBa_K2232014 p43-TSLV1-CA-α Composite Coding region of the pink mutant version of amilCP
BBa_K2232015 P43-OF4-CA-α Composite Coding region of the purple mutant version of amilCP
BBa_K2232026 p43-CA-β Composite Coding region of the green mutant version of amilCP





Improved Parts

This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (in grey) are presented below. You can click on the part number to see the details.


Part Number Name Type Part Description Improvement
BBa_K2232020
BBa_K911008		 PsspB-gerAa Composite Germination-promoting receptor (gerA) expression system Optimize its sequence
BBa_K2232022
BBa_K2465205		 p43-csos3 Composite Carbonic anhydrase (csoS3) expression system Optimize its sequence
BBa_K2232025
BBa_K302035		 mazEF Composite Coding region of Sucrose-limitation induced kill switch Characterize in Bacillus Subtilus