Difference between revisions of "Team:Worldshaper-Nanjing/Composite Part"

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<div class="column full_size judges-will-not-evaluate">
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    <section class="nj-box001 animated fadeInLeft" data-wow-duration="700ms" data-wow-delay="100ms">
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<h3>★  ALERT! </h3>
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        <img src="https://static.igem.org/mediawiki/2017/a/ad/T-Worldshaper-Nanjing-27001.png" alt="" class="bg-stretch" />
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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    </section>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="clear"></div>
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    <div class="gradient_bg">
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        <article class="nj-box002 animated fadeInLeft" data-wow-duration="700ms" data-wow-delay="500ms">
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                                <h2>INP-FBPA</h2>
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                            </div>
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                        </div>
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                                <p>
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                                    BBa_K2513034
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                                </p>
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                                <p>
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                                    INP-FBPA
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                                </p>
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                                <div class="nj-box3">
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                                    <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/d/db/T-Worldshaper-Nanjing-j2002.png" />
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                                </div>
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                                <p>This part encodes a fusion protein that composed of INP and FBPA and designed to be expressed at the surface of E.coli or Synechococcus sp. PCC 7002. We replaced the 6His tag of INP-6His (BBa_K2509019) with a ferric iron binding protein FBPA (BBa_K2513032)to build this part.</p>
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                                <p>BBa_K2509019 and BBa_K2513032</p>
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                                <p>We directly replaced the 6His tag with FBPA without being deleted its stop codon to make a C-fusion protein. So that the whole part can only be transferred as a fusion protein with N part of INP and FBPA.</p>
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                                <p>Results:</p>
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                                <p>Constructions and expression results</p>
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                                <div class="nj-box3">
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                                    <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/08/T-Worldshaper-Nanjing-j2003.png" />
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                                </div>
  
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                                <p>Metal binding result 1</p>
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                                <div class="nj-box3">
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                                    <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/01/T-Worldshaper-Nanjing-j2004.png" />
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                                </div>
  
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                                <p>Metal binding result 2</p>
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                                <div class="nj-box3">
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                                    <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/b/bc/T-Worldshaper-Nanjing-j2005.png" />
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                                </div>
  
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<div class="column full_size">
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<h1>Composite Parts</h1>
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<p>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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</p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<br>
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<h3>Best Composite Part Special Prize</h3>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<br><br>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<br>
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<div class="highlight">
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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Latest revision as of 03:22, 2 November 2017

INP-FBPA

BBa_K2513034

INP-FBPA

This part encodes a fusion protein that composed of INP and FBPA and designed to be expressed at the surface of E.coli or Synechococcus sp. PCC 7002. We replaced the 6His tag of INP-6His (BBa_K2509019) with a ferric iron binding protein FBPA (BBa_K2513032)to build this part.

BBa_K2509019 and BBa_K2513032

We directly replaced the 6His tag with FBPA without being deleted its stop codon to make a C-fusion protein. So that the whole part can only be transferred as a fusion protein with N part of INP and FBPA.

Results:

Constructions and expression results

Metal binding result 1

Metal binding result 2

Copyright © 2017 Team: worldshaper-nanjing All Rights Reserved.