|
|
| (5 intermediate revisions by the same user not shown) |
| Line 34: |
Line 34: |
| | </div> | | </div> |
| | </div> | | </div> |
| − | <nav class="collapse navbar-collapse navbar-right" role="navigation">
| + | <nav class="collapse navbar-collapse navbar-right" role="navigation"> |
| | <div class="main-menu"> | | <div class="main-menu"> |
| | <ul class="nav navbar-nav navbar-right"> | | <ul class="nav navbar-nav navbar-right"> |
| Line 74: |
Line 74: |
| | <li class="dropdown"> | | <li class="dropdown"> |
| | <a class="dropdown-toggle" data-toggle="dropdown"> | | <a class="dropdown-toggle" data-toggle="dropdown"> |
| − | RESULTS | + | Experiments |
| | <span class="caret"></span> | | <span class="caret"></span> |
| | </a> | | </a> |
| Line 80: |
Line 80: |
| | <ul> | | <ul> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Experiments">Experiments</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/NOTEBOOK.html">NOTEBOOK</a> |
| | </li> | | </li> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/PROOF.html">PROOF</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/PROTOCOLS.html">PROTOCOLS</a> |
| | </li> | | </li> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Demonstrate">Demonstrate</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Results">RESULTS</a> |
| | </li> | | </li> |
| | <li> | | <li> |
| Line 101: |
Line 101: |
| | <div class="dropdown-menu"> | | <div class="dropdown-menu"> |
| | <ul> | | <ul> |
| − | <li>
| |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Parts">Parts</a>
| |
| − | </li>
| |
| | <li> | | <li> |
| | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Basic_Part">Basic Parts</a> | | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Basic_Part">Basic Parts</a> |
| Line 115: |
Line 112: |
| | </ul> | | </ul> |
| | </div> | | </div> |
| − | </li>
| |
| − | <li class="dropdown">
| |
| − | <a class="dropdown-toggle" data-toggle="dropdown">
| |
| − | NOTEBOOK
| |
| − | <span class="caret"></span>
| |
| − | </a>
| |
| − | <div class="dropdown-menu">
| |
| − | <ul>
| |
| − | <li>
| |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/NOTEBOOK.html">NOTEBOOK</a>
| |
| − | </li>
| |
| − | <li>
| |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/PROTOCOLS.html">PROTOCOLS</a>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </li>
| |
| − | <li>
| |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Safety">Safety</a>
| |
| | </li> | | </li> |
| | <li class="dropdown"> | | <li class="dropdown"> |
| Line 143: |
Line 121: |
| | <ul> | | <ul> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/HP/Silver">Silver Hp</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/HP/Silver">SILVER PRACTICES</a> |
| | </li> | | </li> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/HP/Gold_Integrated">Integrated and Gold</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/HP/Gold_Integrated">INTEGRATED PRACTICES</a> |
| | </li> | | </li> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Engagement">PUBLIC ENGAGEMENT</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Engagement">EDUCATION & PUBLIC ENGAGEMENT</a> |
| | </li> | | </li> |
| | <li> | | <li> |
| Line 161: |
Line 139: |
| | </li> | | </li> |
| | <li> | | <li> |
| − | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Applied_Design">Applied Design</a> | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Hardware">Hardware</a> |
| | </li> | | </li> |
| | </ul> | | </ul> |
| Line 180: |
Line 158: |
| | <div class="col-md-12"> | | <div class="col-md-12"> |
| | <div class="block"> | | <div class="block"> |
| − | <h2>COMMON Page</h2> | + | <h2>INP-FBPA</h2> |
| | </div> | | </div> |
| | </div> | | </div> |
| Line 190: |
Line 168: |
| | <div class="row"> | | <div class="row"> |
| | <div class="col-md-12"> | | <div class="col-md-12"> |
| − | <div class="nj-box3">
| |
| − | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/03/T-worldshaper-XSHS-00004.jpeg" />
| |
| − | </div>
| |
| | <div class="nj-box4"> | | <div class="nj-box4"> |
| | <p> | | <p> |
| − | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quas sit expedita, iusto repellendus cumque, officia architecto consequatur illo fuga eum sed ut autem eos voluptas. Nemo, a, rem! Atque quisquam aperiam eaque tenetur autem, soluta itaque omnis. Minus nesciunt, sint, animi illum quo ab voluptate esse delectus unde maiores iure, quasi a suscipit ipsam aliquid voluptatem. Perspiciatis eveniet, pariatur illum aut cum dolor neque consequatur error aliquid facilis in quasi temporibus assumenda tempore, doloremque autem saepe enim nihil. Voluptates asperiores ullam voluptate quas similique ratione quia hic, eum distinctio laboriosam, consectetur tempora voluptatibus optio natus cumque est necessitatibus dolore alias. | + | BBa_K2513034 |
| | </p> | | </p> |
| | <p> | | <p> |
| − | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis expedita repellendus laboriosam aliquid. Neque doloribus ea, id reprehenderit alias saepe debitis eligendi molestias odit, nesciunt rem. Dolorem saepe, provident dolore nesciunt laudantium nostrum enim natus veritatis harum maxime et iure ratione, nulla. Minus excepturi commodi tempore voluptate. Blanditiis similique dolor asperiores ex excepturi perspiciatis, dolores id esse. Voluptate beatae nesciunt cum esse ratione officiis necessitatibus blanditiis ea, laboriosam fugit vero maxime? Voluptatum illo dolorum autem pariatur quisquam. Voluptates soluta culpa necessitatibus veritatis tempora incidunt doloribus placeat repellat et facilis eum sapiente fugit numquam aut, laboriosam aspernatur, esse, magnam excepturi repudiandae amet voluptas nulla quidem. Veritatis nisi consequuntur saepe qui quisquam dignissimos assumenda, iusto odio. Dignissimos reprehenderit esse iusto cupiditate nisi enim, animi similique itaque, perspiciatis error qui. Aperiam, architecto provident. | + | INP-FBPA |
| | </p> | | </p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/d/db/T-Worldshaper-Nanjing-j2002.png" /> |
| | + | </div> |
| | + | <p>This part encodes a fusion protein that composed of INP and FBPA and designed to be expressed at the surface of E.coli or Synechococcus sp. PCC 7002. We replaced the 6His tag of INP-6His (BBa_K2509019) with a ferric iron binding protein FBPA (BBa_K2513032)to build this part.</p> |
| | + | <p>BBa_K2509019 and BBa_K2513032</p> |
| | + | <p>We directly replaced the 6His tag with FBPA without being deleted its stop codon to make a C-fusion protein. So that the whole part can only be transferred as a fusion protein with N part of INP and FBPA.</p> |
| | + | <p>Results:</p> |
| | + | <p>Constructions and expression results</p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/08/T-Worldshaper-Nanjing-j2003.png" /> |
| | + | </div> |
| | + | |
| | + | <p>Metal binding result 1</p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/01/T-Worldshaper-Nanjing-j2004.png" /> |
| | + | </div> |
| | + | |
| | + | <p>Metal binding result 2</p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/b/bc/T-Worldshaper-Nanjing-j2005.png" /> |
| | + | </div> |
| | + | |
| | + | |
| | + | |
| | </div> | | </div> |
| | </div> | | </div> |
