Difference between revisions of "Team:BGIC-Union/InterLab"

 
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                     <li><a href="https://2017.igem.org/Team:BGIC-Union/Team">members</a></li>
 
                     <li><a href="https://2017.igem.org/Team:BGIC-Union/Team">members</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Collaborations">collaborations</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Collaborations">collaborations</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Sponsors">sponsors</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Attributions">attributions</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Attributions">attributions</a></li>
 
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                             </ul>
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                             <ul class="dropdown-menu" >
 
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                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Description">description</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Description">description</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Design”></a>design</li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Design">design</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Results">results</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Results">results</a></li>
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                                <li><a href="https://2017.igem.org/Team:BGIC-Union/Model">model</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Demonstrate">demonstrate</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Demonstrate">demonstrate</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Improve">improve</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Improve">improve</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/References">references</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
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                         <li class="dropdown ">    <a href="https://2017.igem.org/Team:BGIC-Union/Lab" class="dropdown-toggle" data-toggle="dropdown"> <b class="caret"></b></a>
 
                         <li class="dropdown ">    <a href="https://2017.igem.org/Team:BGIC-Union/Lab" class="dropdown-toggle" data-toggle="dropdown"> <b class="caret"></b></a>
 
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                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Experiments">experiments</a></li>
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                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Experiments">protocols</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Notebook">notebook</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/Notebook">notebook</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/InterLab">InterLab</a></li></ul>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/InterLab">InterLab</a></li></ul>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Composite_Parts">composite parts</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Composite_Parts">composite parts</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Parts_Collection">parts collection</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Parts_Collection">parts collection</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Measurement">measurement</a></li>
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                             </ul>
 
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                           <li class="dropdown ">  <a href="https://2017.igem.org/Team:BGIC-Union/Practices-Design" class="dropdown-toggle" data-toggle="dropdown"> <b class="caret"></b></a>
 
                           <li class="dropdown ">  <a href="https://2017.igem.org/Team:BGIC-Union/Practices-Design" class="dropdown-toggle" data-toggle="dropdown"> <b class="caret"></b></a>
 
                             <ul class="dropdown-menu" role="menu">
 
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                                <li><a href="https://2017.igem.org/Team:BGIC-Union/Practices-Design">timeline</a></li>
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                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/HP/Silver">silver</a></li>
 
                                 <li><a href="https://2017.igem.org/Team:BGIC-Union/HP/Silver">silver</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/HP/Gold_Integrated">gold</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/HP/Gold_Integrated">gold</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Gold_Integrated">integrated</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Engagement">public engagement</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Engagement">public engagement</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Applied_Design">applied design</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Applied_Design">applied design</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Entrepreneurship">entrepreneurship</a></li>
 
<li><a href="https://2017.igem.org/Team:BGIC-Union/Entrepreneurship">entrepreneurship</a></li>
 
                             </ul>
 
                             </ul>
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                        </li>
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<li class="dropdown ">
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                            <a href="https://2017.igem.org/Team:BGIC-Union/Model">Model </a>
 
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<h2>Team Gathering</h2>
 
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<p>1+1+1+1+1+1+1+1+1+1+1+1>12</br>
 
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Having been through a month of number theory online learning and intense brain storming, the team settled the basic scheme for the project and met up at Shenzhen BGI-college for the first time.
 
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</p>
 
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<span></span>
 
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<div class="read_more" data-id="22/03/2012">Read more</div>
 
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<img src="images/thumbnails/2.jpg" alt="" />
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<h2>JUNE, 12</h2>
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<span></span><div class="read_more" data-id="12/06/2012">Read more</div>
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<div class="item" data-id="21/06/2017" data-description="Lorem ipsum dolor sit amet, consectetur adipiscing elit...">
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<!-- +++++ Projects Section +++++ -->
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<div class="container pt" id="white">
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<div class="row mt">
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<h2>JUNE, 21</h2>
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<div>
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<span></span><div class="read_more" data-id="21/06/2012">Read more</div>
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</br></br></br>We are excited to have a chance to participate in the Fourth International Interlaboratory Study as a team in IGEM, 2017 and contribute our data as part of the GFP measurement. Interlab study requires us to transform eight plasmids into DH5α E.coli cell in order to measure OD 600 and Fluorescence of them as the time passes by. </br></br>
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</div>
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<h4>Calibration:</h4> </br>
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<div class="item_open" data-id="21/06/2017">
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1.Mea sure the Abs600 of both the LUDOX-S40 and water to obtain the correction factor.
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<div class="timeline_open_content">
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</br>
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<h2></h2>
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2.Measure the fluorescence of a series of diluted fluorescein to draw the fluorescein standard curve.
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</div>
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</br>
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</div>
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<h4>Cell Measurement Procedure:</h4>
 +
</br></br>
 +
1. At first, we transform eight plasmids into DH5α E. Coli cell which include Positive control, Negative Control, D1-D6. After 12 hours, we pick one colony from each plate and inoculate each into a 50ml Falcon tube. </br></br>
 +
2. After a 12 hours incubation, we measure the primary OD600 and dilute bacteria solution into an OD600 of 0.02. </br></br>
 +
3. Finally, we measure the OD600 and Fluorescein every 2 hours and record the data into table.
  
 +
<div class="row center">
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<div class="col-md-6">
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<img src="https://static.igem.org/mediawiki/2017/c/c6/BGICINTERLAB1.jpg"width="500px"/>
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</div>
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<div class="col-md-6">
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<img src="https://static.igem.org/mediawiki/2017/c/c6/BGICINTERLAB1.jpg"width="500px"/>
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</div>
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</div>
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<div class="row center">
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<div class="col-md-6">
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<img src="https://static.igem.org/mediawiki/2017/1/17/Interlab3bgic.jpg"width="500px"/>
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</div>
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<div class="col-md-6">
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<img src="https://static.igem.org/mediawiki/2017/a/a4/Bgicinterlab4.jpg"width="500px"/>
 +
</div><div class="row center">
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<div class="col-md-6">
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<img src="https://static.igem.org/mediawiki/2017/0/0a/Bgicinterlab5.jpg"width="500px"/>
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</div>
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<div class="col-md-6">
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<img src="https://static.igem.org/mediawiki/2017/b/bf/Bgicinterlab6.jpg"width="500px"/>
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</div>
  
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</div>
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 +
<h4>Observations</h4> </br></br>
 +
1. It takes us much time to dilute the bacteria solution to the OD600 of 0.02. So the exact value of the concentration of bacteria solution may be incorrect. </br>
 +
2. As a team composed of high school students, sometimes we feel confused in understanding the procedures and the theory behind the instructions. </br>
 +
3. Since our data is very different compared to other teams’, maybe there are some improper operations or other mistakes in our study. </br>
 +
</br></br>
 +
Finally, we sincerely appreciate BGI for permitting us to use the plate reader.
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/ad/Port01.png" width="400px" height="300px"></img></a>
 
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<p>EXTRACT </p>
 
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</div>
 
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<div class="col-lg-4">
 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/29/Port02.png" width="400px" height="300px"></img></a>
 
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<p>CENTRIFUGE</p>
 
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</div>
 
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<div class="col-lg-4">
 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2017/6/6e/Port03.png" width="400px" height="300px"></img></a>
 
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<p>ctDNA AMPLIFICATION</p>
 
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<div class="col-lg-4">
 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2017/c/ce/Port04.png" width="400px" height="300px"></img></a>
 
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<p>DETECTION</p>
 
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</div>
 
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<div class="col-lg-4">
 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/2a/Port05.png" width="400px" height="300px"></img></a>
 
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<p>SIGNALING</p>
 
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</div>
 
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<div class="col-lg-4">
 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2017/6/67/Port06.png" width="400px" height="300px"></img></a>
 
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<p>TEST CHIP</p>
 
 
</div>
 
</div>
 
</div><!-- /row -->
 
</div><!-- /row -->
 
</div><!-- /container -->
 
</div><!-- /container -->
 
 
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<!-- +++++ Footer Section +++++ -->
 
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<div id="footer">
 
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<div class="container">
 
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<div class="row">
 
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<div class="col-lg-4">
 
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    <p>F12 BGI-College，146 Beishan Road，Yantian District 518083
 
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                                    </br> Shenzhen，Guangdong Province, China，Asia
 
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                                </br>Phone:+86-755-36307888
 
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                                </br>Email: info@genomics.cn
 
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</br>Fax:+86-755-36307273</p>
 
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</p>
 
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<div class="col-lg-4">
 
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<h4>Links</h4>
 
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<p>
 
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<a href="#">SAFETY</a><br/>
 
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<a href="#">PARTS</a><br/>
 
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<a href="#">EVALUATION</a>
 
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</p>
 
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</div><!-- /col-lg-4 -->
 
 
 
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<div class="col-lg-4">
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<h4>BGIC-COLLEGE 2017</h4>
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<img src="https://static.igem.org/mediawiki/2017/d/de/Repute-logo-light.png" width="60px" height="72px"></img>
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<p></br><i class="fa-twitter">TWITTER:@BGIC-Union</i> </p>
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Latest revision as of 03:27, 2 November 2017

Team：BGIC-Union




We are excited to have a chance to participate in the Fourth International Interlaboratory Study as a team in IGEM, 2017 and contribute our data as part of the GFP measurement. Interlab study requires us to transform eight plasmids into DH5α E.coli cell in order to measure OD 600 and Fluorescence of them as the time passes by.

Calibration:


1.Mea sure the Abs600 of both the LUDOX-S40 and water to obtain the correction factor.
2.Measure the fluorescence of a series of diluted fluorescein to draw the fluorescein standard curve.

Cell Measurement Procedure:



1. At first, we transform eight plasmids into DH5α E. Coli cell which include Positive control, Negative Control, D1-D6. After 12 hours, we pick one colony from each plate and inoculate each into a 50ml Falcon tube.

2. After a 12 hours incubation, we measure the primary OD600 and dilute bacteria solution into an OD600 of 0.02.

3. Finally, we measure the OD600 and Fluorescein every 2 hours and record the data into table.

Observations



1. It takes us much time to dilute the bacteria solution to the OD600 of 0.02. So the exact value of the concentration of bacteria solution may be incorrect.
2. As a team composed of high school students, sometimes we feel confused in understanding the procedures and the theory behind the instructions.
3. Since our data is very different compared to other teams’, maybe there are some improper operations or other mistakes in our study.


Finally, we sincerely appreciate BGI for permitting us to use the plate reader.