Difference between revisions of "Team:XMU-China/Contribution"

 
(3 intermediate revisions by the same user not shown)
Line 106: Line 106:
 
<!--end title-->
 
<!--end title-->
  
−
<!--left guide-->
+
 
−
<ul class="pageScroll left">
+
<!--cebian guide-->
−
<li class="scrollNav scrollNav_2"><a href="#subtitle1"></a></li>
+
<div id="float">
−
<li class="scrollNav scrollNav_2"><a href="#subtitle2"></a></li>
+
<div id="cebianlan">
−
<li class="scrollNav scrollNav_3"><a href="#subtitle3"></a></li>
+
<div class="menu-list"><a href="#subtitle1">Introduction</a></div>
−
</ul>
+
<div class="menu-list"><a href="#subtitle2">BBa_K1357009</a></div>
−
<!--end left guide-->
+
<div class="menu-list"><a href="#subtitle3">BBa_K540001</a></div>
 +
</div></div>
 +
 
 +
<script type="text/javascript">
 +
window.onload=
 +
function(){
 +
    var oDiv = document.getElementById("float"),
 +
    H = 60,
 +
    Y = oDiv
 +
    while (Y) {H += Y.offsetTop; Y = Y.offsetParent}
 +
    window.onscroll = function()
 +
    {
 +
        var s = document.body.scrollTop || document.documentElement.scrollTop
 +
        if(s>H) {
 +
            oDiv.style = "position:fixed;top:20px;"
 +
        } else {
 +
            oDiv.style = ""
 +
        }
 +
    }
 +
}
 +
</script>
 +
<!--end cebian guide-->
 +
 
  
 
<!--column-->
 
<!--column-->
−
<div class="column" id="column">
+
<div class="column" id="column"><span class="blank" id="subtitle1"></span>
−
<span class="subtitle" id="subtitle1">-----* Introduction *-----</span>
+
<span class="subtitle" >-----* Introduction *-----</span>
 
<p>We also improved the characterization of two existing BioBrick Parts although we've participated in <a href="https://2017.igem.org/Team:XMU-China/InterLab">the InterLab Measurement Study</a>. The parts we tested are <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> and <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a>. </p>
 
<p>We also improved the characterization of two existing BioBrick Parts although we've participated in <a href="https://2017.igem.org/Team:XMU-China/InterLab">the InterLab Measurement Study</a>. The parts we tested are <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> and <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a>. </p>
−
<span class="blank"></span>
+
<span class="blank" id="subtitle2"></span>
−
<span class="subtitle" id="subtitle2">-----* <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> *-----</span>
+
<span class="subtitle">-----* <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> *-----</span>
 
<p>The fluorescent protein is used widly as a reporter, such as GFP and RFP. To enrich the kinds of fluorescent proteins, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP. <br />Firstly, we got <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grew on the plate.</p><br />
 
<p>The fluorescent protein is used widly as a reporter, such as GFP and RFP. To enrich the kinds of fluorescent proteins, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP. <br />Firstly, we got <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grew on the plate.</p><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
Line 125: Line 147:
 
<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>
−
<span class="blank"></span>
+
<span class="blank" id="subtitle3"></span>
−
<span class="subtitle" id="subtitle3">-----* <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> *-----</span>
+
<span class="subtitle">-----* <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> *-----</span>
 
<p>Meanwhile, we also tested <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> using the reporter <a href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a>. The Cobalt induced promoter <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> was added before the reporter <a href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a>, then the constructed plasmid was transformed into E.coli DH5α to measure the fluorescence intensity and OD600</p><br />
 
<p>Meanwhile, we also tested <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> using the reporter <a href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a>. The Cobalt induced promoter <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> was added before the reporter <a href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a>, then the constructed plasmid was transformed into E.coli DH5α to measure the fluorescence intensity and OD600</p><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/8/8c/XMU-2017-Cobalt.jpeg"></div><br /><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/8/8c/XMU-2017-Cobalt.jpeg"></div><br /><br />

Latest revision as of 03:27, 2 November 2017

2017.igem.org/Team:XMU-China/Contribution

-----* Introduction *-----

We also improved the characterization of two existing BioBrick Parts although we've participated in the InterLab Measurement Study. The parts we tested are BBa_K1357009 and BBa_K540001.

-----* BBa_K1357009 *-----

The fluorescent protein is used widly as a reporter, such as GFP and RFP. To enrich the kinds of fluorescent proteins, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.
Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grew on the plate.




Secondly, we extracted the plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter BBa_J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.


-----* BBa_K540001 *-----

Meanwhile, we also tested BBa_K540001 using the reporter BBa_I13504. The Cobalt induced promoter BBa_K540001 was added before the reporter BBa_I13504, then the constructed plasmid was transformed into E.coli DH5α to measure the fluorescence intensity and OD600




The result shows that there is not a specific relationship with the concentration of Cobalt. There might be some problems with this promoter.