Difference between revisions of "Team:XMU-China/Contribution"

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{{XMU-China}}
 
 
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<title>2017.igem.org/Team:XMU-China/Contribution</title>
  
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Contribution</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<ul class="menu">
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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<a href="https://2017.igem.org/Team:XMU-China"><img src="https://static.igem.org/mediawiki/2017/9/9a/T--XMU-China--iconhome.png"></a></li>
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<a>PROJECT</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/Description">Description</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Design">Design</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Results">Results</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Applied_Design">Applied&nbsp;Design</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Parts">Parts</a></li>
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<li class="dropdown" id="Notebook">
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<a>NOTEBOOK</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Engineering">Engineering</a></li>
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<a>HARDWARE</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/Hardware">Overview</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Freeze-Dry">Freeze-Dry</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/East-Wind">East-Wind</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Accessories">Accessories</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/More_Chips">More&nbsp;Chips</a></li>
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<a>MODEL</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/Model">Overview</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Modeling">Modeling</a></li>
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<a>HUMAN&nbsp;PRACTICES</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/Human_Practices">Overview</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Engagement">Engagement</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
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<a>OTHER&nbsp;WORK</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/InterLab">Interlab</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Contribution">Contribution</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Improve">Improve</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Measurement">Measurement</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Safety">Safety</a></li>
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<li class="dropdown" id="Team">
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<a>TEAM</a>
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<li><a href="https://2017.igem.org/Team:XMU-China/Team">Team</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Judging">Judging</a></li>
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<div class="menu-list"><a href="#subtitle1">Introduction</a></div>
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<div class="menu-list"><a href="#subtitle2">BBa_K1357009</a></div>
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<div class="menu-list"><a href="#subtitle3">BBa_K540001</a></div>
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<span class="subtitle" >-----* Introduction *-----</span>
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<p>We also improved the characterization of two existing BioBrick Parts although we've participated in <a href="https://2017.igem.org/Team:XMU-China/InterLab">the InterLab Measurement Study</a>. The parts we tested are <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> and <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a>. </p>
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<span  class="blank" id="subtitle2"></span>
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<span class="subtitle">-----* <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> *-----</span>
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<p>The fluorescent protein is used widly as a reporter, such as GFP and RFP. To enrich the kinds of fluorescent proteins, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the <strong>coral Acropora millepora, amilCP</strong>. In this year, we want to verify the characteristic of BFP. <br />Firstly, we got <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grew on the plate.</p><br />
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<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
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<p>Secondly, we extracted the plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a> with the promoter <a href="http://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>, then transformed into E.coli DH5α again. We observed the <strong>strong blue just one day later</strong>. The result shows that <a href="http://parts.igem.org/Part:BBa_K1357009">BBa_K1357009</a> works well. </p><br />
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<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
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<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>
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<span  class="blank" id="subtitle3"></span>
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<span class="subtitle">-----* <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> *-----</span>
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<p>Meanwhile, we also tested <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> using the reporter <a href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a>. The Cobalt induced promoter <a href="http://parts.igem.org/Part:BBa_K540001">BBa_K540001</a> was added before the reporter <a href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a>, then the constructed plasmid was transformed into E.coli DH5α to measure the fluorescence intensity and OD600</p><br />
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<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/8/8c/XMU-2017-Cobalt.jpeg"></div><br /><br />
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<p>The result shows that there is not a specific relationship with the concentration of Cobalt. There might be some problems with this promoter.</p>
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<p>Xiamen University, Fujian, China<br />
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No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005</p>
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Latest revision as of 03:27, 2 November 2017

2017.igem.org/Team:XMU-China/Contribution

-----* Introduction *-----

We also improved the characterization of two existing BioBrick Parts although we've participated in the InterLab Measurement Study. The parts we tested are BBa_K1357009 and BBa_K540001.

-----* BBa_K1357009 *-----

The fluorescent protein is used widly as a reporter, such as GFP and RFP. To enrich the kinds of fluorescent proteins, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.
Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grew on the plate.




Secondly, we extracted the plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter BBa_J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.


-----* BBa_K540001 *-----

Meanwhile, we also tested BBa_K540001 using the reporter BBa_I13504. The Cobalt induced promoter BBa_K540001 was added before the reporter BBa_I13504, then the constructed plasmid was transformed into E.coli DH5α to measure the fluorescence intensity and OD600




The result shows that there is not a specific relationship with the concentration of Cobalt. There might be some problems with this promoter.