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<h4><strong>1.1 SDS-PAGE verification for <I>OprF-3*LBT 4</I> & <I>OprF-Sitag</I> expression</strong> </h4> | <h4><strong>1.1 SDS-PAGE verification for <I>OprF-3*LBT 4</I> & <I>OprF-Sitag</I> expression</strong> </h4> | ||
− | <p>Whether the | + | <p>Whether the oprF-3*LBT or oprF-Sitag can be expressed or not needs to be detected by SDS-PAGE.</p> |
<img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b7/2017_HUST_China_demostrate002.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b7/2017_HUST_China_demostrate002.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/c/ce/2017_HUST_China_demostrate003.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/c/ce/2017_HUST_China_demostrate003.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | ||
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<div class="col-xs-12"> | <div class="col-xs-12"> | ||
− | <p>Firstly, we need to identify that <I>OprF-3* LBT4</I> and <I> | + | <p>Firstly, we need to identify that <I>OprF-3* LBT4</I> and <I>OprF-Sitag</I> are expressed on the surface of the E. coli. We centrifugated the bacteria solution and got the whole test BL21 cells induced by IPTG. As control, we also extracted the not induced BL21 cells. Then we separate the protein of the whole cell by SDS-PAGE. </p> |
− | <p>Figure 1 shows an obvious ~33kDa protein bands in test lane, which cannot be found in the control lane. This result proves that the test BL21 can produce | + | <p>Figure 1 shows an obvious ~33kDa protein bands in test lane, which cannot be found in the control lane. This result proves that the test BL21 can produce OprF-3* LBT 4. </p> |
− | <p>Figure 2 shows an obvious ~57kDa protein bands in test lane, which cannot be found in the not induced lane. This result proves that the test BL21 can produce | + | <p>Figure 2 shows an obvious ~57kDa protein bands in test lane, which cannot be found in the not induced lane. This result proves that the test BL21 can produce OprF-Sitag. </p> |
</div> | </div> | ||
Revision as of 03:28, 2 November 2017