Difference between revisions of "Team:XMU-China/Safety"

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{{XMU-China}}
 
 
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<title>2017.igem.org/Team:XMU-China/Safety</title>
  
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<h1> Safety </h1>
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<body style="background-color:#FFFFFF;" background="https://static.igem.org/mediawiki/2017/c/cb/T--XMU-China--homebackground.png";>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<a href="https://2017.igem.org/Team:XMU-China"><img src="https://static.igem.org/mediawiki/2017/9/9a/T--XMU-China--iconhome.png"></a></li>
  
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<li class="dropdown" id="Project">
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<a>PROJECT</a>
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<ul style="dropdown-menu">
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<li><a href="https://2017.igem.org/Team:XMU-China/Description">Description</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Design">Design</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Results">Results</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Applied_Design">Applied&nbsp;Design</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Parts">Parts</a></li>
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</ul>
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<h5>Safe Project Design</h5>
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<a>NOTEBOOK</a>
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<ul style="dropdown-menu">
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<li><a href="https://2017.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Engineering">Engineering</a></li>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li class="dropdown" id="Hardware">
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<a>HARDWARE</a>
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<ul style="dropdown-menu">
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<li><a href="https://2017.igem.org/Team:XMU-China/Hardware">Overview</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Freeze-Dry">Freeze-Dry</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/East-Wind">East-Wind</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Accessories">Accessories</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/More_Chips">More&nbsp;Chips</a></li>
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</ul>
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</li>
  
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<ul>
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<li class="dropdown" id="Model">
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<li>Choosing a non-pathogenic chassis</li>
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<a>MODEL</a>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<ul style="dropdown-menu">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Model">Overview</a></li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Modeling">Modeling</a></li>
 
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</li>
  
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<li class="dropdown" id="HP">
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<a>HUMAN&nbsp;PRACTICES</a>
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<ul style="dropdown-menu">
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<li><a href="https://2017.igem.org/Team:XMU-China/Human_Practices">Overview</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Engagement">Engagement</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
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</ul>
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<li class="dropdown" id="other">
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<a>OTHER&nbsp;WORK</a>
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<ul style="dropdown-menu">
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<li><a href="https://2017.igem.org/Team:XMU-China/InterLab">Interlab</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Contribution">Contribution</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Improve">Improve</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Measurement">Measurement</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Safety">Safety</a></li>
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</ul>
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<li class="dropdown" id="Team">
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<a>TEAM</a>
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<ul style="dropdown-menu">
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<li><a href="https://2017.igem.org/Team:XMU-China/Team">Team</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
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<li><a href="https://2017.igem.org/Team:XMU-China/Judging">Judging</a></li>
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</ul>
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</li> 
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<h5>Safe Lab Work</h5>
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<img class="titleimg"  src="https://static.igem.org/mediawiki/2017/e/ed/T--XMU-China--safetybg.png" />
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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<div class="menu-list"><a href="#subtitle1">Safe Project Design</a></div>
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<div class="menu-list"><a href="#subtitle2">Safe Lab Work</a></div>
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<div class="menu-list"><a href="#subtitle3">Safe Shipment</a></div>
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<div class="subtitle">-----------* Safe Project Design *-----------</div>
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<p>Firstly, <strong>E.coli BL21</strong> and <strong>E.coli DH5α</strong> were the only chassis when we performed the molecular cloning and expression. Our bacteria will be engineered to detect metal ions, e.g., iron and mercury. We propose to construct an intermediate to normalize the response level of various ions. An induced expression system will be used to amplify the signal response to those ions.For toxic substances, we have strict operational requirements. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. Besides, the main part of our product is reusable. </p><br />
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<span class="safetyimg"><img class="safetyimgone" src="https://static.igem.org/mediawiki/2017/8/83/T--XMU-China--Safety-picture1.jpeg"></span>
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<span class="blank"  id="subtitle2"></span>
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<div class="subtitle" >---------------* Safe Lab Work *---------------</div>
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<p>The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment, first aid kit and so on. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. When using <strong>arsenic-containing reagents</strong> for experiments, must wear double gloves, goggles, masks, and must operate on special test benches. We handled all the biological materials, wastes and equipments strictly following the <strong>BSL-1 requirements</strong>. Turn off the water and electricity, close window and door when we leave the lab.</p><br />
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<span class="safetyimg"><img class="safetyimgone" style="border:none;" src="https://static.igem.org/mediawiki/2017/5/57/T--XMU-China--Safety-picture2.jpeg"></span>
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<span  class="blank" id="subtitle3"></span>
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<div class="subtitle" >---------------* Safe Shipment *---------------</div>
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<p>We sent our BioBricks through the standard shipping process required by iGEM.</p>
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<span class="blank"></span>
 
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<h5>Safe Shipment</h5>
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<p>Xiamen University, Fujian, China<br />
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No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005</p>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 03:30, 2 November 2017

2017.igem.org/Team:XMU-China/Safety

-----------* Safe Project Design *-----------

Firstly, E.coli BL21 and E.coli DH5α were the only chassis when we performed the molecular cloning and expression. Our bacteria will be engineered to detect metal ions, e.g., iron and mercury. We propose to construct an intermediate to normalize the response level of various ions. An induced expression system will be used to amplify the signal response to those ions.For toxic substances, we have strict operational requirements. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. Besides, the main part of our product is reusable.


---------------* Safe Lab Work *---------------

The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment, first aid kit and so on. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. When using arsenic-containing reagents for experiments, must wear double gloves, goggles, masks, and must operate on special test benches. We handled all the biological materials, wastes and equipments strictly following the BSL-1 requirements. Turn off the water and electricity, close window and door when we leave the lab.


---------------* Safe Shipment *---------------

We sent our BioBricks through the standard shipping process required by iGEM.