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− | <img id="background-image" src="https://static.igem.org/mediawiki/2017/6/69/WHU-China-child-bg.jpeg" alt=""/>
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− | </div>
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− | <div id="header">
| + | <h1>Designs</h1> |
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| + | <ul id="cont"> |
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− | <ul class="nv">
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− | <li id="nv0" style="width:200px"><a href="https://2017.igem.org/Team:WHU-China"></a></li>
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− | <li id="nv1">
| + | <h2>1.Increase the production of B12 in <i>B. megaterium</i></h2> |
− | <a>Project</a>
| + | <h3>1.1 Increase flux of glutamyl-tRNA to uroporphyrinogen Ⅲ by regulating feedback inhibition of glutamyl-tRNA reductase (HemA) </h3> |
− | <ul>
| + | <h3>1.2 Reduce flux of uroporphyrinogen Ⅲ to heme by an antisense RNA(as) strategy </h3> |
− | <li id="nv11"><a href="https://2017.igem.org/Team:WHU-China/Overview">Overview</a></li>
| + | <h3>1.3 Overexpress RdhANP to soak up excess B12 production </h3> |
− | <li id="nv12"><a href="https://2017.igem.org/Team:WHU-China/Background">Background</a></li>
| + | <h2>2.Express and verify RdhANP</h2> |
− | <li id="nv13"><a href="https://2017.igem.org/Team:WHU-China/Introduction">Introduction</a></li>
| + | <h3>2.1 Clone <i>rdhANP</i> to suitable vectors </h3> |
− | <li id="nv14"><a href="https://2017.igem.org/Team:WHU-China/Designs">Designs</a></li>
| + | <h3>2.2 Induce to express RdhANP in <i>B. megaterium</i></h3> |
− | <li id="nv15"><a href="https://2017.igem.org/Team:WHU-China/Experiments">Experiments</a></li>
| + | <h3>2.3 Purify and concentrate RdhANP </h3> |
− | <li id="nv16"><a href="https://2017.igem.org/Team:WHU-China/Results">Results</a></li>
| + | <h3>2.4 Construct dehalogenation-testing system <i>in vitro</i> and <i>in vivo</i></h3> |
− | </ul>
| + | <h3>2.5 Detect dehalogenation reaction by HPLC-MS</h3> |
− | </li>
| + | <h3>2.6 Overexpress RdhANP by testing different vectors </h3> |
− | <li id="nv2"><a>Parts</a>
| + | <h3>2.7 Design synthetic RBS by 'Ribosome binding site calculator' and RBSDesigner and test their production level</h3> |
− | <ul>
| + | <h3>2.8 Perform Directed Enzyme& Protein Evolution by certain companies. </h3> |
− | <li id="nv21"><a href="https://2017.igem.org/Team:WHU-China/Description_Part">Description</a></li>
| + | <h3>2.9 Test the dehalogenation of these mutants our constructed wastewater-imitating system </h3> |
− | <li id="nv22"><a href="https://2017.igem.org/Team:WHU-China/Basic_Part">Basic</a></li>
| + | <h3>2.10 Coordinate B12 production and RdhANP expression</h3> |
− | <li id="nv23"> <a href="https://2017.igem.org/Team:WHU-China/Composite_Part">Composite</a></li>
| + | <h2>3.Bind <i>B. megaterium</i> to cellulose membrane</h2> |
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| + | <h3>3.1 Coexpress Cellulose binding domain(CBD) and Membrane anchoring domain(MAD) in <i>B. megaterium</i></h3> |
− | </ul>
| + | <h3>3.2 Test the binding of <i>B. megaterium</i> to cellulose membrane</h3> |
− |
| + | <h2>4.Coexpress CBD, MAD and RdhANP in <i>B. megaterium</i> and test its binding to cellulose membrane and dehalogenation</h2> |
− | </li>
| + | <h2>5.Construct biofilm containing RdhANP-expressing <i>B. megaterium</i></h2> |
− | <li id="nv3"><a>Interlab</a>
| + | <h2>6.Introduce our engineered biofilm and cellulose membrane to HMBR</h2> |
− | <ul>
| + | <h2>7.Cooperate with JLU-iGEM to complete the halogenated phenol's degradation pathway and optimize organohalide-contained wastewater treatment</h2> |
− | <li id="nv31"><a href="https://2017.igem.org/Team:WHU-China/Results">Results</a></li>
| + | <h2>8.Introduce downstream devices to eliminate microorganisms leaked into the effluent</h2> |
− | <li id="nv32"><a href="https://2017.igem.org/Team:WHU-China/Calibration">Calibration</a></li>
| + | <h2></h2><h2></h2> |
− | <li id="nv33"><a href="https://2017.igem.org/Team:WHU-China/Cell_Measuremnet">CellMeasuremnet</a></li>
| + | </ul> |
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| + | |
− | </ul>
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− |
| + | |
− | </li>
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− | <li id="nv4">
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− | <a>Notebook</a>
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− | <ul>
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− | <li id="nv41"><a href="https://2017.igem.org/Team:WHU-China/Dry_Lab">DryLab</a></li>
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− | <li id="nv42"><a href="https://2017.igem.org/Team:WHU-China/Wet_Lab">WetLab</a></li>
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− | </ul>
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− | </li>
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− | <li id="nv5">
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− | <a>Model</a>
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− | <ul>
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− | <li id="nv51"><a href="https://2017.igem.org/Team:WHU-China/Model">Modeling</a></li>
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− | <li id="nv52"><a href="https://2017.igem.org/Team:WHU-China/Hardware">Hardware</a></li>
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− | </ul>
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− | </li>
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− |
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− | <li id="nv6">
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− | <a>Human Practice</a>
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− | <ul>
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− | <li id="nv61"><a href="https://2017.igem.org/Team:WHU-China/HP/Silver">Silver</a></li>
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− | <li id="nv62"><a href="https://2017.igem.org/Team:WHU-China/HP/Gold_Integrated">Gold&Integrated</a></li>
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− | <li id="nv63"><a href="https://2017.igem.org/Team:WHU-China/Engagement">Engagement</a></li>
| + | |
− | </ul>
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− | </li>
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− | <li id="nv7"><a>Safety</a>
| + | |
− | <ul>
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− | <li id="nv71"><a href="https://2017.igem.org/Team:WHU-China/Project_Design">ProjectDesign</a></li>
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− | <li id="nv72"><a href="https://2017.igem.org/Team:WHU-China/Lab_Work">LabWork</a></li>
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− |
| + | |
− | </ul>
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− | </li>
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− | <li id="nv8"><a>Team</a>
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− | <ul>
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− | <li id="nv81"><a href="https://2017.igem.org/Team:WHU-China/Member">Member</a></li>
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− | <li id="nv82"><a href="https://2017.igem.org/Team:WHU-China/Attributions">Attributions</a> </li>
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− | <li id="nv83"><a href="https://2017.igem.org/Team:WHU-China/Collaborations">Collaborations</a></li>
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− |
| + | |
− | </ul>
| + | |
− | </li>
| + | |
− | <li id="nv9"><a>Achievement</a>
| + | |
− | <ul>
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− | <li id="nv91"><a href="https://2017.igem.org/Team:WHU-China/Judging_Form">JudgingForm</a></li>
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− | <li id="nv92"><a href="https://2017.igem.org/Team:WHU-China/Applied_Design">AppliedDesign</a> </li>
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− | <li id="nv93"><a href="https://2017.igem.org/Team:WHU-China/Enterpreneurship">Enterpreneurship</a></li>
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− |
| + | |
− | </ul>
| + | |
− | </li>
| + | |
− | </ul>
| + | |
− | </div>
| + | |
| </div> | | </div> |
| </div> | | </div> |
| | | |
− | <h3>1.Increase the production of B12 in B. megaterium</h3>
| |
− | 1.1 Increase flux of glutamyl-tRNA to uroporphyrinogen Ⅲ by regulating feedback inhibition of glutamyl-tRNA reductase (HemA)
| |
− | 1.2 Reduce flux of uroporphyrinogen Ⅲ to heme by an antisense RNA(as) strategy
| |
− | 1.3 Overexpress RdhANP to soak up excess B12 production
| |
− | <h1>2.Express and verify RdhANP</h1>
| |
− | 2.1 Clone rdhANP to suitable vectors
| |
− | 2.2 Induce to express RdhANP in B. megaterium
| |
− | 2.3 Purify and concentrate RdhANP
| |
− | 2.4 Construct dehalogenation-testing system in vitro and in vivo
| |
− | 2.5 Detect dehalogenation reaction by HPLC-MS
| |
− | 2.6 Overexpress RdhANP by testing different vectors
| |
− | 2.7 Design synthetic RBS by 'Ribosome binding site calculator' and RBSDesigner and test their production level
| |
− | 2.8 Perform Directed Enzyme& Protein Evolution by certain companies.
| |
− | 2.9 Test the dehalogenation of these mutants our constructed wastewater-imitating system
| |
− | 2.10 Coordinate B12 production and RdhANP expression
| |
− | <h1>3.Bind B. megaterium to cellulose membrane</h1>
| |
− | 3.1 Coexpress Cellulose binding domain(CBD) and Membrane anchoring domain(MAD) in B. megaterium
| |
− | 3.2 Test the binding of B. megaterium to cellulose membrane
| |
− | <h1>4.Coexpress CBD, MAD and RdhANP in B. megaterium and test its binding to cellulose membrane and dehalogenation</h1>
| |
− | <h1>5.Construct biofilm containing RdhANP-expressing B. megaterium</h1>
| |
− | <h1>6.Introduce our engineered biofilm and cellulose membrane to HMBR</h1>
| |
− | <h3>7.Cooperate with JLU-iGEM to complete the halogenated phenol's degradation pathway and optimize organohalide-contained wastewater treatment</h3>
| |
− | <h1>8.Introduce downstream devices to eliminate microorganisms leaked into the effluent</h1>
| |
| | | |
− | </p>
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| </body> | | </body> |
| </html> | | </html> |