Difference between revisions of "Team:Shanghaitech/Achievements"

 
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<h1>Achievements</h1>
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<h2>Bronze</h2>
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<p><strong> Register The Team </strong></p>
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<p>We have registered a great team and had a great summer for processing our project. For now we are now looking forward to the Giant Jamboree.</p>
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<p><strong> Deliverables  </strong></p>
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<p>We have filled out all the forms and pages that are required carefully. Also, we prepared our poster and presentation to better show you guys about our project.</p>
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<p><strong> Attribution </strong></p>
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<p>The attributions page on our team wiki page contains the attributions of all the works done for our project, clearly distinguishing the work done by the students of our team and others.</p>
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<p><a href='https://2017.igem.org/Team:Shanghaitech/Attributions' target='_blank' >https://2017.igem.org/Team:Shanghaitech/Attributions</a></p>
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<p><strong> Interlab </strong></p>
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<p>We have participated in the Interlab Measurement Study this tear and got a serious of reliable and restrict data.</p>
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<p><a href='https://2017.igem.org/Team:Shanghaitech/InterLab' target='_blank' >https://2017.igem.org/Team:Shanghaitech/InterLab</a></p>
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<h2>Silver</h2>
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<p><strong> New Part Submission </strong></p>
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<p>This year we have constructed a lot of parts, here we show the three best new parts we did: </p><p>The first one is ‘Rpa-Las converter’ , which can convert Rpa molecules into Las molecules.</p><p>(<a href='http://parts.igem.org/Part:BBa_K2315046' target='_blank' >http://parts.igem.org/Part:BBa_K2315046</a>)<p>The second one is ‘LasR+pLas-GFP HSL inducible fluorescent actuator', which can be induced by several AHLs and test their crosstalk.</p><p>(<a href='http://parts.igem.org/Part:BBa_K2315034' target='_blank' >http://parts.igem.org/Part:BBa_K2315034</a>) </p><p>The last one is ‘Las molecule generator’, which can produce Las 3OC12-HSL without inducer. </p><p>(<a href='http://parts.igem.org/Part:BBa_K2315033' target='_blank' >http://parts.igem.org/Part:BBa_K2315033</a>)</p><p>We have successfully characterized these three new parts and submitted the data to the main page. Finally, we have submitted them to the iGEM Parts Registry on time.</p>
 
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<p><strong> Collaboration </strong></p>
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<p>We have collaborated with other iGEM teams in meaningful ways and made contributions to projects of other teams.</p>
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<p>* We worked as a leader and collaborated with other 10 iGEM teams to take videos about biosafety.</p>
 
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<p>* We provided USTC a kind of reagent – CdS nanoparticles for testing whether CdS nanoparticles can increase the cathode-current as expected. Eventually, we received their feedback of their data.</p>
<div class="bg-primary">
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<p>* We helped UCAS characterize their nitrogen promoter to make their data more solid.</p>
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<p>* We visited Fudan and finally gave them a realistic model for their project.</p>
<section>
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<p>* We have collected parts from other teams to help us for constructing our devices library.</p>
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<p>* We have attended many conferences and have meetings with many iGEM teams. We have participated in CCiC in FAFU, and one of our team member, Mingzhe Chen, has be invited to give a short introduction about quorum sensing system.</p>
<center>
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<p><a href='https://2017.igem.org/Team:Shanghaitech/Collaborations' target='_blank' >https://2017.igem.org/Team:Shanghaitech/Collaborations</a></p>
            <img src="https://static.igem.org/mediawiki/2016/1/1c/T--Imperial_College--medalsbanner.png" height="300"/>
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<p><strong>Human Practice </strong></p>
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<p>The great amount of work we have done for Human Practices has given rise to the increasing interests of Synthetic Biology of at least hundreds of adults from different fields. Firstly,we have done public education: In this part, we brought synthetic biology and MagicBlock to the public. We visited high schools to introduce synthetic biology to the students and to guide them to design bio-product using MagicBlock. We also used Social media to publicize synthetic biology and our project. Secondly, we leaded biosafety videos for 10 iGEM teams: Biosafety is one of the most important issues everyone should be aware of before starting any biology related experiments. However, the biosafety education in China is far behind the fast development of modern biological research. In some places, students are not trained properly because of the outdated education material or the language barrier that prevent them from reading biosafety guidelines written in English.</p>
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<p><a href='https://2017.igem.org/Team:Shanghaitech/HP/Silver' target='_blank' >https://2017.igem.org/Team:Shanghaitech/HP/Silver</a></p>
 
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<h2>Gold</h2>
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<p><strong> Integrated Human Practice </strong></p>
 
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<p>Our integrated human practice constitutively guides our project in various ways:</p>
<br><br><br>
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<p>From one hand, inspired by the creativity and enthusiasm of non-biologists through interviews and questionnaires, we have found that many people are very enthusiastic and imaginative about bio-designs, yet have little knowledge in synthetic biology and little lab experience.</p>
<specialh3>Jamboree Results</specialh3><br><br>
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<p>During the invention of MagicBlock, we maintain contact with the public, to test MagicBlock as well as receiving suggestions. we held two workshops, guiding participants to use MagicBlock to design their own bio-products. We received various thought-provoking designs using MagicBlock elements, such as water pollution alarm, synthetic music, and bacteria painting panel .et al, all originated from non-biologists.</p>
<p><i class="fa fa-trophy fa-2x fa-fw"></i> Grand Prize Winner Undergraduate <br>
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<p>During this practice, we received valuable suggestions to improve MagicBlock .</p>
<i class="fa fa-trophy fa-2x fa-fw"></i>Best Foundational Advance Project <br>
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<p>Very importantly, we have received professional insights from professors, which provide direction for follow-up work including human practice, hardware and software design, and modeling.</p>
<i class="fa fa-trophy fa-2x fa-fw"></i>Best Wiki  <i class="fa fa-smile-o fa-fw"></i><br>
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<p><a href='https://2017.igem.org/Team:Shanghaitech/HP/Gold_Integrated' target='_blank' >https://2017.igem.org/Team:Shanghaitech/HP/Gold_Integrated</a></p>
<i class="fa fa-trophy fa-2x fa-fw"></i>Best Poster <br>
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<p><strong> Improved Basic Parts </strong></p>
<i class="fa fa-trophy fa-2x fa-fw"></i>Best Education & Public Engagement <br>
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<p>We have improved two basic parts (LasI/LasR coding sequence) and integrated their function. The data of improved parts and the systems of integrated parts can be found through the following link:</p>
<i class="fa fa-trophy fa-2x fa-fw"></i>Best New Basic Part - STAR <a href="http://parts.igem.org/Part:BBa_K1893010">(BBa_K1893010) </a><br><br>
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<p><a href='http://parts.igem.org/Part:BBa_C0179' target='_blank' >http://parts.igem.org/Part:BBa_C0179</a></p>
 
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<p><a href='http://parts.igem.org/Part:BBa_C0178' target='_blank' >http://parts.igem.org/Part:BBa_C0178</a></p>
<i class="fa fa-hand-lizard-o fa-2x fa-fw"></i>Nominated for Best New Composite Part - Arabinose inducible gp2 <a href="http://parts.igem.org/Part:BBa_K1893016" >(BBa_K1893016) </a> <br>
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<p><strong> Modeling </strong></p>
<i class="fa fa-hand-peace-o fa-2x fa-fw"></i>Nominated for Best Model <br>
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<p>We simplify the actual biology process into basic model that only remains input molecule, promotor, transcription gene, mRNA, goal protein and output molecule from both dynamic perspective and responding ability. In developed model, we consider different conditions including the population growth, diffusion of signal and decay of signal molecules in cells. which will have influence on our block. Finally, we completely construct the model of our block, which will instruct our experiment results and using of our system. Moreover, this model does some basic researches on population and new measurement methods.</p>
<i class="fa fa-coffee fa-2x fa-fw"></i>Nominated for Best Integrated Human Practices <br><br><br>
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<p>The detailed information can be found through the following link:</p>
 
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<p><a href='https://2017.igem.org/Team:Shanghaitech/Model' target='_blank' >https://2017.igem.org/Team:Shanghaitech/Model</a></p>
</div>
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<p><strong> Demonstrate  Our Work </strong></p>
 
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<p>In the result section, we have showed that each of our parts (hardware, software, wet lab, model and human practice) work beautifully as expected! Therefore, we claim optimistically that our prototype MagicBlock platform has proved the concept in engaging more people to practice synthetic biology!</p>
<div class="col-lg-6">
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<p><a href="https://2017.igem.org/Team:Shanghaitech/Demonstrate">https://2017.igem.org/Team:Shanghaitech/Demonstrate</a></p>
<br><br><br>
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<img src="https://static.igem.org/mediawiki/2016/5/5e/T--Imperial_College--JamboreePosterPic.png" width="320"/>
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<img src="https://static.igem.org/mediawiki/2016/1/10/T--Imperial_College--JamboreePresPic.png" width="320"/><br>
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<img src="https://static.igem.org/mediawiki/2016/3/3b/T--Imperial_College--JamboreePrizePic.png"width="310"/>
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<img src="https://static.igem.org/mediawiki/2016/0/06/T--Imperial_College--JamboreeEngPic.png" width="330"/><br>
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<p> Jamboree Pictures (Photo Credit to iGEM Foundation and Justin Knight)</p>
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<center><a href="https://2016.igem.org/Team:Imperial_College/Team"><img src="https://static.igem.org/mediawiki/2016/3/38/T--Imperial_College--JamboreePic.png" height="400"/></a></center>
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<specialh3>Bronze</specialh3>
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<p><i class="fa fa-check fa-2x fa-fw" ></i>Registered for iGEM, had a great summer, and plan to attend the Giant Jamboree.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Documented our project on our Team Wiki page.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Plan to present a poster of our project and talk at the Giant Jamboree.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Attributed all of the work done for our project correctly. <a href="https://2016.igem.org/Team:Imperial_College/Attributions"> <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Imperial_College--ReadMore.jpg" width="100"/></a> <br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Created and documented Part pages on the Registry for the Parts we have made.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Submitted DNA samples of your new Parts to the Registry.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Completed safety forms for safety check in, about our lab, about our project and our final safety form.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Completed the judging form. <br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Documented at least one new standard BioBrick Part or Device central to our project and submit this part to the iGEM Registry. <a href="http://parts.igem.org/Part:BBa_K1893010">BBa_K1893010 </a>(STAR) <a href="http://parts.igem.org/Part:BBa_K1893009">BBa_K1893009 </a> (STAR Reporter)  <a href="http://parts.igem.org/Part:BBa_K1893016" >BBa_K1893016 </a>(AraBAD + gp2)).<br> </p>
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<specialh3>Silver</specialh3>
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<p><i class="fa fa-check fa-2x fa-fw" ></i>Validated Part: (<a href="http://parts.igem.org/Part:BBa_K1893010">BBa_K1893010 </a>(STAR) <a href="http://parts.igem.org/Part:BBa_K1893009">BBa_K1893009 </a> (STAR Reporter)  <a href="http://parts.igem.org/Part:BBa_K1893016" >BBa_K1893016 </a>(AraBAD + gp2)).<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Collaboration: This year we have collaborated with 12 iGEM teams, details of which can be found on our collaborations page <a href="https://2016.igem.org/Team:Imperial_College/Collaborations"> <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Imperial_College--ReadMore.jpg" width="100"/></a><br> Some of our contributions include filling in surveys for LMU, TU Munich, Paris Saclay and the UCL iGEM teams and providing technical support and equipment for the City of London high school team.<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Human Practices: <a href="https://2016.igem.org/Team:Imperial_College/HP/Silver"> <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Imperial_College--ReadMore.jpg" width="100"/></a><br>
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<specialh3>Gold</specialh3>
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<p><i class="fa fa-check fa-2x fa-fw" ></i>Integrated Human Practices <a href="https://2016.igem.org/Team:Imperial_College/HP/Gold"> <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Imperial_College--ReadMore.jpg" width="100"/></a><br>
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<i class="fa fa-check fa-2x fa-fw" ></i>Improve a previous part or project: We have improved 5 parts in our project. All of our parts can be found here: </p><ul style="font-size:16px;">
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<li><a href="http://parts.igem.org/Part:BBa_C0062">BBa_C0062</a> - luxR repressor/activator </li>
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<li><a href="http://parts.igem.org/Part:BBa_C0071">BBa_C0071</a> - rhlR repressor/activator from P. aeruginosa PA3477 </li>
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<li><a href="http://parts.igem.org/Part:BBa_C0079">BBa_C0079</a> - lasR activator from P. aeruginosa PAO1 </li>
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<li><a href="http://parts.igem.org/Part:BBa_C0077">BBa_C0077</a> - cinR activator from Rhizobium leguminosarum </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1321333">BBa_K1321333</a> - pSB-AraC-pBAD </li>
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</ul>
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<br>
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<i class="fa fa-check fa-2x fa-fw" ></i>In our proof of concept, we transformed two cell populations with different chromoproteins. The cells expressing the chromoproteins were mixed to form new colours from various co-cultures. We also found that different chromoproteins placed varied loads on the the cell populations, resulting in different growth rates, so that, if grown together, one coloured population would likely outcompete the other. Our Genetically Engineered Artificial Ratio (G.E.A.R.) system could work to maintain the colour of the co-culture by slowing the growth of the faster growing population.<a href="https://2016.igem.org/Team:Imperial_College/Proof"> <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Imperial_College--ReadMore.jpg" width="100"/></a><br>
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Latest revision as of 03:37, 2 November 2017

Bronze

Register The Team

We have registered a great team and had a great summer for processing our project. For now we are now looking forward to the Giant Jamboree.

Deliverables

We have filled out all the forms and pages that are required carefully. Also, we prepared our poster and presentation to better show you guys about our project.

Attribution

The attributions page on our team wiki page contains the attributions of all the works done for our project, clearly distinguishing the work done by the students of our team and others.

https://2017.igem.org/Team:Shanghaitech/Attributions

Interlab

We have participated in the Interlab Measurement Study this tear and got a serious of reliable and restrict data.

https://2017.igem.org/Team:Shanghaitech/InterLab

Silver

New Part Submission

This year we have constructed a lot of parts, here we show the three best new parts we did:

The first one is ‘Rpa-Las converter’ , which can convert Rpa molecules into Las molecules.

(http://parts.igem.org/Part:BBa_K2315046)

The second one is ‘LasR+pLas-GFP HSL inducible fluorescent actuator', which can be induced by several AHLs and test their crosstalk.

(http://parts.igem.org/Part:BBa_K2315034)

The last one is ‘Las molecule generator’, which can produce Las 3OC12-HSL without inducer.

(http://parts.igem.org/Part:BBa_K2315033)

We have successfully characterized these three new parts and submitted the data to the main page. Finally, we have submitted them to the iGEM Parts Registry on time.

Collaboration

We have collaborated with other iGEM teams in meaningful ways and made contributions to projects of other teams.

* We worked as a leader and collaborated with other 10 iGEM teams to take videos about biosafety.

* We provided USTC a kind of reagent – CdS nanoparticles for testing whether CdS nanoparticles can increase the cathode-current as expected. Eventually, we received their feedback of their data.

* We helped UCAS characterize their nitrogen promoter to make their data more solid.

* We visited Fudan and finally gave them a realistic model for their project.

* We have collected parts from other teams to help us for constructing our devices library.

* We have attended many conferences and have meetings with many iGEM teams. We have participated in CCiC in FAFU, and one of our team member, Mingzhe Chen, has be invited to give a short introduction about quorum sensing system.

https://2017.igem.org/Team:Shanghaitech/Collaborations

Human Practice

The great amount of work we have done for Human Practices has given rise to the increasing interests of Synthetic Biology of at least hundreds of adults from different fields. Firstly,we have done public education: In this part, we brought synthetic biology and MagicBlock to the public. We visited high schools to introduce synthetic biology to the students and to guide them to design bio-product using MagicBlock. We also used Social media to publicize synthetic biology and our project. Secondly, we leaded biosafety videos for 10 iGEM teams: Biosafety is one of the most important issues everyone should be aware of before starting any biology related experiments. However, the biosafety education in China is far behind the fast development of modern biological research. In some places, students are not trained properly because of the outdated education material or the language barrier that prevent them from reading biosafety guidelines written in English.

https://2017.igem.org/Team:Shanghaitech/HP/Silver

Gold

Integrated Human Practice

Our integrated human practice constitutively guides our project in various ways:

From one hand, inspired by the creativity and enthusiasm of non-biologists through interviews and questionnaires, we have found that many people are very enthusiastic and imaginative about bio-designs, yet have little knowledge in synthetic biology and little lab experience.

During the invention of MagicBlock, we maintain contact with the public, to test MagicBlock as well as receiving suggestions. we held two workshops, guiding participants to use MagicBlock to design their own bio-products. We received various thought-provoking designs using MagicBlock elements, such as water pollution alarm, synthetic music, and bacteria painting panel .et al, all originated from non-biologists.

During this practice, we received valuable suggestions to improve MagicBlock .

Very importantly, we have received professional insights from professors, which provide direction for follow-up work including human practice, hardware and software design, and modeling.

https://2017.igem.org/Team:Shanghaitech/HP/Gold_Integrated

Improved Basic Parts

We have improved two basic parts (LasI/LasR coding sequence) and integrated their function. The data of improved parts and the systems of integrated parts can be found through the following link:

http://parts.igem.org/Part:BBa_C0179

http://parts.igem.org/Part:BBa_C0178

Modeling

We simplify the actual biology process into basic model that only remains input molecule, promotor, transcription gene, mRNA, goal protein and output molecule from both dynamic perspective and responding ability. In developed model, we consider different conditions including the population growth, diffusion of signal and decay of signal molecules in cells. which will have influence on our block. Finally, we completely construct the model of our block, which will instruct our experiment results and using of our system. Moreover, this model does some basic researches on population and new measurement methods.

The detailed information can be found through the following link:

https://2017.igem.org/Team:Shanghaitech/Model

Demonstrate Our Work

In the result section, we have showed that each of our parts (hardware, software, wet lab, model and human practice) work beautifully as expected! Therefore, we claim optimistically that our prototype MagicBlock platform has proved the concept in engaging more people to practice synthetic biology!

https://2017.igem.org/Team:Shanghaitech/Demonstrate