Difference between revisions of "Team:Oxford/Parts"

 
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{{Oxford}}
 
{{Oxford}}
 
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<body>
 
<div class="container">   
 
<div class="container">   
 
     <div style="margin-top: 100px"></div>
 
     <div style="margin-top: 100px"></div>
 
<h1>Parts</h1>
 
<h1>Parts</h1>
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<h2>Parts for the Cell-Free System</h2>
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<img class="img-responsive img-center" width="200px;" src="https://static.igem.org/mediawiki/2017/8/84/Parts_page.png">
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<h2>Parts for the DNA System</h2>
 
<div id="cell-free-parts-table">
 
<div id="cell-free-parts-table">
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<table class="table table-hover">
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<table class="table table-hover" style="margin-left:-40px;">
 
     <thead>
 
     <thead>
 
       <tr>
 
       <tr>
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         <td>mCherry - TEV protease (non-cleaving)</td>
 
         <td>mCherry - TEV protease (non-cleaving)</td>
 
         <td>Doesn’t self-cleave</td>
 
         <td>Doesn’t self-cleave</td>
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         <td>?</td>
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         <td>BBa_J06504; BBa_K1319004</td>
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         <td>?</td>
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         <td>Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/</td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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     <td>BBa_K2450201</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2450201">BBa_K2450201</a></td>
 
     <td>C200</td>
 
     <td>C200</td>
 
     <td>Modified TetR with TEV cleavage site - CFP</td>
 
     <td>Modified TetR with TEV cleavage site - CFP</td>
 
     <td>TEV cleavage site in linker between DNA binding and dimerisation domains</td>
 
     <td>TEV cleavage site in linker between DNA binding and dimerisation domains</td>
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     <td>BBa_K106669; BBa_K???????</td>
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     <td>BBa_K106669</td>
 
     <td>TEV cleavage site from Merck; CFP from AddGene</td>
 
     <td>TEV cleavage site from Merck; CFP from AddGene</td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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      <td>BBa_K2450251</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2450251">BBa_K2450251</a></td>
 
      <td>C250</td>
 
      <td>C250</td>
 
      <td>TetR - CFP</td>
 
      <td>TetR - CFP</td>
 
      <td>Normal TetR Class B</td>
 
      <td>Normal TetR Class B</td>
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      <td>BBa_K106669; BBa_K???????</td>
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      <td>BBa_K106669</td>
 
      <td>CFP from AddGene</td>
 
      <td>CFP from AddGene</td>
 
  </tr>
 
  </tr>
 
  <tr>
 
  <tr>
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      <td>BBa_K2450301</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2450301">BBa_K2450301</a></td>
 
      <td>C300</td>
 
      <td>C300</td>
 
      <td>Tet promoter - RBS - YFP</td>
 
      <td>Tet promoter - RBS - YFP</td>
 
      <td>DNA part, contains promoter and RBS to give signal of YFP</td>
 
      <td>DNA part, contains promoter and RBS to give signal of YFP</td>
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      <td>BBa_R0040</td>
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      <td>BBa_R0040; BBa_B0030</td>
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      <td>?</td>
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      <td>eYFP from AddGene</td>
 
  </tr>
 
  </tr>
 
     </tbody>
 
     </tbody>
 
   </table>
 
   </table>
 
   </div>
 
   </div>
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   <h2>Parts for the OMV System</h2>
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   <h2>Parts for the Protein-Based System</h2>
 
   <div id="omv-parts-table">
 
   <div id="omv-parts-table">
 
   <table class="table table-hover">
 
   <table class="table table-hover">
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     <tbody>
 
     <tbody>
 
       <tr>
 
       <tr>
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         <td>BBa_K2450401</td>
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         <td><a href="http://parts.igem.org/Part:BBa_K2450401">BBa_K2450401</a></td>
 
         <td>V100</td>
 
         <td>V100</td>
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         <td>N-SpyCatcher - OmpA - sfGFP</td>
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         <td>SpyTag - OmpA - sfGFP</td>
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         <td>SpyCatcher is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm</td>
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         <td>SpyTag is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm</td>
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         <td>?</td>
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         <td>n/a</td>
 
         <td>Alves et al, 2016 AddGene</td>
 
         <td>Alves et al, 2016 AddGene</td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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     <td>BBa_K2450451</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2450451">BBa_K2450451</a></td>
 
     <td>v150</td>
 
     <td>v150</td>
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     <td>OmpA - C-SpyCatcher - sfGFP</td>
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     <td>OmpA - SpyTag - sfGFP</td>
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     <td>SpyCatcher is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm</td>
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     <td>SpyTag is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm</td>
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     <td>?</td>
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     <td>n/a</td>
 
     <td>Alves et al, 2016</td>
 
     <td>Alves et al, 2016</td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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      <td>BBa_K2450501</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2450501">BBa_K2450501</a></td>
 
      <td>v200</td>
 
      <td>v200</td>
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      <td>SpyTag - sfGFP - Quencher</td>
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      <td>TorA Leader Sequence - SpyCatcher - sfGFP - Quencher</td>
 
      <td>TEV cleavage site between sfGFP and the quenching peptide</td>
 
      <td>TEV cleavage site between sfGFP and the quenching peptide</td>
 
      <td>BBa_K1319014</td>
 
      <td>BBa_K1319014</td>
 
      <td>Alves et al, 2016</td>
 
      <td>Alves et al, 2016</td>
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  </tr>
 
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  <tr>
 
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      <td>BBa_K2450502</td>
 
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      <td>V200noQ</td>
 
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      <td>SpyTag - sfGFP</td>
 
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      <td>Control to check localisation to OMVs</td>
 
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      <td>?</td>
 
−
      <td>Alves et al, 2016</td>
 
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  </tr>
 
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    <tr>
 
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      <td>BBa_K2450701</td>
 
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      <td>V500</td>
 
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      <td>sfGFP - N-terminal split TEV Protease - Inhibited Coiled Coil</td>
 
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      <td>Inhibited coiled coil contains a TEV cleavage site between the two helicies, leaving the acid coiled coil attached to the N-terminal split protease</td>
 
−
      <td>?</td>
 
−
      <td>Alves et al, 2016 Split TEV paper</td>
 
−
  </tr>
 
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    <tr>
 
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      <td>BBa_K2450702</td>
 
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      <td>V600</td>
 
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      <td>mCherry - Inhibited Coiled Coil - C-terminal Split TEV Protease</td>
 
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      <td>Inhibited coiled coil contains a TEV protease cleavage site. This will result in the cleavage of the mCherry - Acid Coil being cleaved from the Base Coil - C Terminal Split TEV Protease.</td>
 
−
      <td>?</td>
 
−
      <td>Alves et al, 2016 Split TEV paper</td>
 
 
  </tr>
 
  </tr>
 
     </tbody>
 
     </tbody>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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</div>
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</html>
 
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{{Oxford/footer}}

Latest revision as of 03:41, 2 November 2017

Parts

Parts for the DNA System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450101 C100 mCherry - TEV protease (non-cleaving) Doesn’t self-cleave BBa_J06504; BBa_K1319004 Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/
BBa_K2450201 C200 Modified TetR with TEV cleavage site - CFP TEV cleavage site in linker between DNA binding and dimerisation domains BBa_K106669 TEV cleavage site from Merck; CFP from AddGene
BBa_K2450251 C250 TetR - CFP Normal TetR Class B BBa_K106669 CFP from AddGene
BBa_K2450301 C300 Tet promoter - RBS - YFP DNA part, contains promoter and RBS to give signal of YFP BBa_R0040; BBa_B0030 eYFP from AddGene

Parts for the Protein-Based System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450401 V100 SpyTag - OmpA - sfGFP SpyTag is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm n/a Alves et al, 2016 AddGene
BBa_K2450451 v150 OmpA - SpyTag - sfGFP SpyTag is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm n/a Alves et al, 2016
BBa_K2450501 v200 TorA Leader Sequence - SpyCatcher - sfGFP - Quencher TEV cleavage site between sfGFP and the quenching peptide BBa_K1319014 Alves et al, 2016