Difference between revisions of "Team:Oxford/Parts"

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<h1>Parts</h1>
 
<h1>Parts</h1>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<img class="img-responsive img-center" width="200px;" src="https://static.igem.org/mediawiki/2017/8/84/Parts_page.png">
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h2>Parts for the DNA System</h2>
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<div id="cell-free-parts-table">
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<table class="table table-hover" style="margin-left:-40px;">
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    <thead>
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      <tr>
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        <th>Registry Number</th>
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        <th>Code used in Lab</th>
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        <th>Brief Description</th>
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        <th>Key Details</th>
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        <th>Biobricks used</th>
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        <th>Other sequence sources</th>
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      </tr>
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    </thead>
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    <tbody>
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      <tr>
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        <td><a href="http://parts.igem.org/Part:BBa_K2450101">BBa_K2450101</a></td>
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        <td>C100</td>
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        <td>mCherry - TEV protease (non-cleaving)</td>
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        <td>Doesn’t self-cleave</td>
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        <td>BBa_J06504; BBa_K1319004</td>
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        <td>Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/</td>
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      </tr>
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      <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2450201">BBa_K2450201</a></td>
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    <td>C200</td>
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    <td>Modified TetR with TEV cleavage site - CFP</td>
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    <td>TEV cleavage site in linker between DNA binding and dimerisation domains</td>
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    <td>BBa_K106669</td>
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    <td>TEV cleavage site from Merck; CFP from AddGene</td>
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      </tr>
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      <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2450251">BBa_K2450251</a></td>
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      <td>C250</td>
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      <td>TetR - CFP</td>
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      <td>Normal TetR Class B</td>
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      <td>BBa_K106669</td>
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      <td>CFP from AddGene</td>
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  </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2450301">BBa_K2450301</a></td>
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      <td>C300</td>
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      <td>Tet promoter - RBS - YFP</td>
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      <td>DNA part, contains promoter and RBS to give signal of YFP</td>
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      <td>BBa_R0040; BBa_B0030</td>
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      <td>eYFP from AddGene</td>
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  </tr>
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    </tbody>
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  </table>
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  </div>
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  <h2>Parts for the Protein-Based System</h2>
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  <div id="omv-parts-table">
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  <table class="table table-hover">
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    <thead>
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      <tr>
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        <th>Registry Number</th>
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        <th>Code used in Lab</th>
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        <th>Brief Description</th>
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        <th>Key Details</th>
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        <th>Biobricks used</th>
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        <th>Other sequence sources</th>
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      </tr>
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    </thead>
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    <tbody>
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      <tr>
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        <td><a href="http://parts.igem.org/Part:BBa_K2450401">BBa_K2450401</a></td>
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        <td>V100</td>
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        <td>SpyTag - OmpA - sfGFP</td>
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        <td>SpyTag is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm</td>
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        <td>n/a</td>
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        <td>Alves et al, 2016 AddGene</td>
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      </tr>
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      <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2450451">BBa_K2450451</a></td>
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    <td>v150</td>
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    <td>OmpA - SpyTag - sfGFP</td>
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    <td>SpyTag is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm</td>
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    <td>n/a</td>
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    <td>Alves et al, 2016</td>
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      </tr>
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      <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2450501">BBa_K2450501</a></td>
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      <td>v200</td>
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      <td>TorA Leader Sequence - SpyCatcher - sfGFP - Quencher</td>
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      <td>TEV cleavage site between sfGFP and the quenching peptide</td>
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      <td>BBa_K1319014</td>
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      <td>Alves et al, 2016</td>
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  </tr>
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    </tbody>
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  </table>
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  </div>
 
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 Oxford</groupparts>
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{{Oxford/footer}}

Latest revision as of 03:41, 2 November 2017

Parts

Parts for the DNA System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450101 C100 mCherry - TEV protease (non-cleaving) Doesn’t self-cleave BBa_J06504; BBa_K1319004 Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/
BBa_K2450201 C200 Modified TetR with TEV cleavage site - CFP TEV cleavage site in linker between DNA binding and dimerisation domains BBa_K106669 TEV cleavage site from Merck; CFP from AddGene
BBa_K2450251 C250 TetR - CFP Normal TetR Class B BBa_K106669 CFP from AddGene
BBa_K2450301 C300 Tet promoter - RBS - YFP DNA part, contains promoter and RBS to give signal of YFP BBa_R0040; BBa_B0030 eYFP from AddGene

Parts for the Protein-Based System

Registry Number Code used in Lab Brief Description Key Details Biobricks used Other sequence sources
BBa_K2450401 V100 SpyTag - OmpA - sfGFP SpyTag is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm n/a Alves et al, 2016 AddGene
BBa_K2450451 v150 OmpA - SpyTag - sfGFP SpyTag is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm n/a Alves et al, 2016
BBa_K2450501 v200 TorA Leader Sequence - SpyCatcher - sfGFP - Quencher TEV cleavage site between sfGFP and the quenching peptide BBa_K1319014 Alves et al, 2016