|
|
(64 intermediate revisions by 2 users not shown) |
Line 1: |
Line 1: |
| {{IISER-Mohali-INDIA}} | | {{IISER-Mohali-INDIA}} |
| <html> | | <html> |
− | <head>
| + | <head> |
| + | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> |
| + | </head> |
| <meta name="description" content="chart created using amCharts live editor" /> | | <meta name="description" content="chart created using amCharts live editor" /> |
| | | |
Line 332: |
Line 334: |
| color:#2d2d2d; | | color:#2d2d2d; |
| @include opacity(1.0); | | @include opacity(1.0); |
| + | }.container { |
| + | width:92%; |
| + | margin-left:2%; |
| + | |
| + | padding-top: 2%; |
| + | font-weight:normal; |
| + | |
| } | | } |
− | .main{ | + | .page_logo{float:right; |
− | box-sizing:border-box;
| + | text-align:right; |
− | width:100%;
| + | margin-right:-20%; |
− | padding:20px; | + | width:35%; |
− | border:1px;
| + | height:35%; |
− | background:transparent;
| + | position:static; |
| + | margin-top:8% |
| + | } |
| + | header, section, article { |
| + | display:block;} |
| + | .page_head{ |
| + | position:static; |
| + | margin-top:; |
| + | text-align:center; |
| + | } |
| + | h1, h2, h3,h5,h6{ |
| + | padding-left: 5%; |
| + | padding-right: 5%; |
| + | font-weight: normal; |
| } | | } |
| + | h4{ |
| + | padding-left: 10%; |
| + | padding-right: 5%; |
| + | font-weight:normal; |
| + | } |
| + | |
| + | |
| + | |
| + | |
| </style> | | </style> |
| + | </head> |
| + | |
| <body> | | <body> |
| + | <div class="container"> |
| + | <header> |
| + | <a href="index.html"><img src="https://static.igem.org/mediawiki/2017/7/7b/T--IISER-Mohali-INDIA--logogeco.png" style="float: left; width:8%; padding-top: 1%;" alt="gEco"></a> |
| + | |
| + | <a href="https://www.facebook.com/igemiiserm/"><img src="https://static.igem.org/mediawiki/2017/5/5f/T--IISER-Mohali-INDIA--FB.png" style="float: right; width: 4%; margin-right: 1%; padding-top: 1%;"></a> |
| + | <a href="https://www.instagram.com/igem_iisermohali/"><img src="https://static.igem.org/mediawiki/2017/c/ce/T--IISER-Mohali-INDIA--Insta.png" style="float: right; width: 4%; margin-right: 1%; padding-top: 1%;"></a> |
| + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/d/d7/T--IISER-Mohali-INDIA--G%2B.png" style="float: right; width: 4%; margin-right: 1%; padding-top: 1%;"></a> |
| + | <a href="https://twitter.com/iGEMIISERM"><img src="https://static.igem.org/mediawiki/2017/0/04/T--IISER-Mohali-INDIA--Tw.png" style="float: right; width: 4%; margin-right: 1%; padding-top: 1%;"></a> |
| + | <a href="https://www.youtube.com/channel/UCXhpctjIXTHUpVCwq_ctgDg"><img src="https://static.igem.org/mediawiki/2017/3/3a/T--IISER-Mohali-INDIA--You.png" style="float: right; width: 4%; margin-right: 1%; padding-top: 1%;"></a> |
| + | </header> |
| + | <div class="page_logo"> |
| + | <img src="https://static.igem.org/mediawiki/2017/a/a4/T--IISER-Mohali-INDIA--interlab.png" width="100%" height="100%"></div> |
| + | |
| + | <article> |
| + | <div class="page-title"><h5>Interlab</h5> |
| + | </div> <br /><br /><br /><br /><br /><br /> |
| + | <section> |
| + | <h3>iGEM's interlab study aims to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. This means teams have to use the same exact protocol around the world to produce common, comparable units for measuring GFP with different plate readers. For more information on the protocol, check out iGEM's official Interlab Study webpage. Below is the data that we collected for the Interlab Study: |
| + | </h3></section> |
| <br /> | | <br /> |
− | <br /> | + | <section> |
− | <br /> | + | <center><table width="90%"> |
− | <br /> | + | <thead> |
− | <div class="main">
| + | <th> |
− | <h3> | + | |
| OD600 Reference Point | | OD600 Reference Point |
− | </h3> | + | </th> |
− | <table> | + | <th> |
| + | Plate Pattern |
| + | <br/> |
| + | This diagram depicts how our samples were organized on our plate:</th></thead> |
| + | <tr> |
| + | <td> |
| + | <table height="100%" width="90%"> |
| <thead> | | <thead> |
| <tr> | | <tr> |
Line 388: |
Line 446: |
| <td>Corrected Abs600</td> | | <td>Corrected Abs600</td> |
| <td>0.0102</td> | | <td>0.0102</td> |
| + | <td></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>Reference OD600</td> | | <td>Reference OD600</td> |
− | <td>0.0425</td> | + | <td>0.0425</td><td></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td>OD600/Abs600</td> | | <td>OD600/Abs600</td> |
− | <td>4.166666667</td> | + | <td>4.166666667</td><td></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | <br /> | + | </td> |
− | <div class="table-responsive"> | + | |
− | <table class="table table-condensed table-hover"> | + | <td> |
− | <thead>
| + | <table height="100%" width="100%"> |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | <th> </th>
| + | |
− | </thead>
| + | |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
Line 504: |
Line 554: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </td></tr></table></center> |
| + | </section> |
| + | <br /> |
| | | |
− | </div>
| + | <section> |
− | | + | |
− | <h2>
| + | |
− | Fluorescence Standard Curves
| + | |
− | </h2>
| + | |
− | <br />
| + | |
− | <h3>uM Fluorescein</h3>
| + | |
− | <table>
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>uM Fluorescein</th>
| + | |
− | <th>50.00</th>
| + | |
− | <th>25</th>
| + | |
− | <th>12.5</th>
| + | |
− | <th>6.25</th>
| + | |
− | <th>3.125</th>
| + | |
− | <th>1.5625</th>
| + | |
− | <th>0.78125</th>
| + | |
− | <th>0.390625</th>
| + | |
− | <th>0.1953125</th>
| + | |
− | <th>0.09765625</th>
| + | |
− | <th>0.048828125</th>
| + | |
− | <th>0</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>Replicate 1</td>
| + | |
− | <td>60105</td>
| + | |
− | <td>43268</td>
| + | |
− | <td>29004</td>
| + | |
− | <td>17671</td>
| + | |
− | <td>9656</td>
| + | |
− | <td>4867</td>
| + | |
− | <td>2466</td>
| + | |
− | <td>1328</td>
| + | |
− | <td>658</td>
| + | |
− | <td>324</td>
| + | |
− | <td>160</td>
| + | |
− | <td>6</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 2</td>
| + | |
− | <td>64328</td>
| + | |
− | <td>45105</td>
| + | |
− | <td>30375</td>
| + | |
− | <td>18411</td>
| + | |
− | <td>9892</td>
| + | |
− | <td>4742</td>
| + | |
− | <td>2569</td>
| + | |
− | <td>1361</td>
| + | |
− | <td>698</td>
| + | |
− | <td>339</td>
| + | |
− | <td>172</td>
| + | |
− | <td>6</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 3</td>
| + | |
− | <td>64879</td>
| + | |
− | <td>46230</td>
| + | |
− | <td>30542</td>
| + | |
− | <td>18336</td>
| + | |
− | <td>9472</td>
| + | |
− | <td>4772</td>
| + | |
− | <td>2540</td>
| + | |
− | <td>1353</td>
| + | |
− | <td>703</td>
| + | |
− | <td>349</td>
| + | |
− | <td>179</td>
| + | |
− | <td>5</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 4</td>
| + | |
− | <td>64574</td>
| + | |
− | <td>46956</td>
| + | |
− | <td>30906</td>
| + | |
− | <td>18065</td>
| + | |
− | <td>9491</td>
| + | |
− | <td>5071</td>
| + | |
− | <td>2587</td>
| + | |
− | <td>1374</td>
| + | |
− | <td>709</td>
| + | |
− | <td>359</td>
| + | |
− | <td>190</td>
| + | |
− | <td>6</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Arith. Mean</td>
| + | |
− | <td>63471.5</td>
| + | |
− | <td>45389.75</td>
| + | |
− | <td>30206.75</td>
| + | |
− | <td>18120.75</td>
| + | |
− | <td>9627.75</td>
| + | |
− | <td>4863</td>
| + | |
− | <td>2540.5</td>
| + | |
− | <td>1354</td>
| + | |
− | <td>692</td>
| + | |
− | <td>342.75</td>
| + | |
− | <td>175.25</td>
| + | |
− | <td>5.75</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Arith. Std.Dev.</td>
| + | |
− | <td>2255.620905</td>
| + | |
− | <td>1606.452069</td>
| + | |
− | <td>831.9172134</td>
| + | |
− | <td>334.6444631</td>
| + | |
− | <td>194.5805317</td>
| + | |
− | <td>148.550777</td>
| + | |
− | <td>53.30728531</td>
| + | |
− | <td>19.37352145</td>
| + | |
− | <td>23.10844002</td>
| + | |
− | <td>14.93039406</td>
| + | |
− | <td>12.57974563</td>
| + | |
− | <td>0.5</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </table>
| + | |
− | <br />
| + | |
| <h3>uM Fluorescein/a.u.</h3> | | <h3>uM Fluorescein/a.u.</h3> |
− | <br /> | + | <center><table width="90%"><tr><td> |
− | <table class="table table-condensed table-hover"> | + | <table width="100%" height="100%"> |
| <thead> | | <thead> |
| <tr> | | <tr> |
Line 675: |
Line 609: |
| </tbody> | | </tbody> |
| </table> | | </table> |
| + | </td></tr></table></center> |
| + | </section> |
| + | <br/> |
| | | |
| + | <section> |
| + | <h6 class="page-title"> Curves<br /><br /><br /> |
| + | <center><div id="chart1div" style="width: 80%; height: 500px; background-color: #282828;"></div><br /> |
| + | <div id="chart2div" style="width: 80%; height: 500px; background-color: #282828;"></div> |
| + | </center> |
| | | |
− | </div> | + | </section><br /> |
− | <div id="chart1div" style="width: 50%; height: 400px; background-color: #282828;" ></div>
| + | <section><h3> Abs600 Raw Readings </h3> |
− | <br /> | + | <h4> These tables summarize our finalized sets of Absorbance reading data over an incubation period of 6 hours with samples measured every 2 hours for a total of 4 time points: </h4> |
− | <div id="chart2div" style="width: 50%; height: 400px; background-color: #282828;" ></div> | + | </section> |
− | <div class="col-md-12 col-xs-12 text-center">
| + | |
− | <h2> Abs600 Raw Readings </h2>
| + | |
− | <h5> These tables summarize our finalized sets of Absorbance reading data over an incubation period of 6 hours with samples measured every 2 hours for a total of 4 time points: </h5> | + | |
| | | |
− | </div>
| + | <section> |
− | <div class="col-md-6 col-xs-12 text-center">
| + | <h4>Hour 0</h4> |
− | <h3>Hour 0</h3> | + | |
| | | |
− | <div> | + | <center><div style="width:80%;"> |
| <table> | | <table> |
| <thead> | | <thead> |
| <tr> | | <tr> |
− | <th>Hour 0:(C-colony, R-replicate)</th> | + | <th>Hour 0:</th> |
| <th>Neg. Control</th> | | <th>Neg. Control</th> |
| <th>Pos. Control</th> | | <th>Pos. Control</th> |
Line 780: |
Line 718: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center> |
− | <div>
| + | </section> |
− | <h3>Hour 2</h3> | + | <h4>Hour 2</h4> |
| + | <center><div style="width:80%;"> |
| + | |
| | | |
− | <div>
| + | |
| <table> | | <table> |
| <thead> | | <thead> |
Line 802: |
Line 742: |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
− | <td]>C1, R1</td> | + | <td>C1, R1</td> |
| | | |
| <td>0.1289</td> <td>0.1165</td> <td>0.0932</td> <td>0.1002</td> <td>0.1056</td> <td>0.0952</td> | | <td>0.1289</td> <td>0.1165</td> <td>0.0932</td> <td>0.1002</td> <td>0.1056</td> <td>0.0952</td> |
Line 808: |
Line 748: |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <td\>C1, R2</td> | + | <td>C1, R2</td> |
| | | |
| <td>0.1294</td> <td>0.1158</td> <td>0.0975</td> <td>0.1031</td> <td>0.1087</td> | | <td>0.1294</td> <td>0.1158</td> <td>0.0975</td> <td>0.1031</td> <td>0.1087</td> |
Line 850: |
Line 790: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center> |
− | <div> | + | </section> |
− | <h3>Hour 4</h3>
| + | |
| | | |
− | <div> | + | <section> |
| + | <h4>Hour 4</h4> |
| + | <center><div style="width:80%;"> |
| + | |
| <table> | | <table> |
| <thead> | | <thead> |
Line 921: |
Line 864: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center> |
− | | + | </section> |
− | </div>
| + | <section> |
− | <div>
| + | <h4>Hour 6</h4> |
− | <h3>Hour 6</h3>
| + | <center> <div style="width:80%;"> |
| + | |
| | | |
− | <div>
| |
| <table> | | <table> |
| <thead> | | <thead> |
Line 994: |
Line 937: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center></section> |
− | </div>
| + | <section> |
− | <div class="col-md-12 col-xs-12 text-center"> | + | <div> |
− | <h2> Fluorescence Raw Readings </h2> | + | <h3> Fluorescence Raw Readings </h3> |
− | <h5> These tables summarize our finalized sets of Fluorescent reading data over an incubation period of 6 hours: </h5> | + | <h4> These tables summarize our finalized sets of Fluorescent reading data over an incubation period of 6 hours: </h5> |
| | | |
| | | |
| </div> | | </div> |
− | <div>
| + | </section> |
− | <h3>Hour 0</h3>
| + | <section> |
| + | <h4>Hour 0</h4> |
| + | <center> <div align="center" style="width:80%;"> |
| | | |
− | <div>
| + | |
| + | |
| <table> | | <table> |
| <thead> | | <thead> |
Line 1,072: |
Line 1,018: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center></section> |
− | </div>
| + | <section> |
− | <div> | + | <h4>Hour 2</h4> |
− | <h3>Hour 2</h3>
| + | <center><div style="width:80%;"> |
| | | |
− | <div>
| + | |
| + | |
| <table> | | <table> |
| <thead> | | <thead> |
Line 1,144: |
Line 1,091: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center> |
− | </div>
| + | </section> |
− | <div> | + | <section><h4>Hour 4</h4> |
− | <h3>Hour 4</h3>
| + | <center><div style="width:80%;"> |
| | | |
− | <div>
| + | |
| + | |
| <table> | | <table> |
| <thead> | | <thead> |
Line 1,216: |
Line 1,164: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center> |
− | </div> | + | </section> |
− | <div>
| + | <section><h4>Hour 6</h4> |
− | <h3>Hour 6</h3>
| + | <center><div style="width:80%;"> |
| | | |
− | <div>
| + | |
| + | |
| <table> | | <table> |
| <thead> | | <thead> |
Line 1,288: |
Line 1,237: |
| </tbody> | | </tbody> |
| </table> | | </table> |
− | </div> | + | </div></center> |
| + | </section><br /><br /> |
| + | </article> |
| | | |
− | </div>
| + | |
| + | </div> |
| </body> | | </body> |
| </html> | | </html> |