Difference between revisions of "Team:Amazonas Brazil/Project"

 
(25 intermediate revisions by 6 users not shown)
Line 63: Line 63:
  
 
   <header>
 
   <header>
−
     <nav class="navbar navbar-fixed-top navbar-default" style="background-color: rgba(255, 255, 255, 0);">
+
     <nav class="navbar navbar-fixed-top navbar-default" id="#" style="background-color: rgba(255, 255, 255, 0);">
 
       <ul class="Links_bar" style="background: linear-gradient(to left, #263D9E, #3F2759);opacity:0.75;">
 
       <ul class="Links_bar" style="background: linear-gradient(to left, #263D9E, #3F2759);opacity:0.75;">
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
Line 69: Line 69:
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
−
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">Design</a></li>
+
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">Applied Design</a></li>
 
       </ul>
 
       </ul>
  
Line 204: Line 204:
  
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
−
               src="https://static.igem.org/mediawiki/2017/4/4a/Amazonas_Brazil_Project_5_1.png">
+
               src="https://static.igem.org/mediawiki/2017/b/b6/Amazonas_Brazil_Substitui%C3%A7%C3%A3o_Design.png">
  
 
             <!--  <p class="p">Adicionar aqui</p> -->
 
             <!--  <p class="p">Adicionar aqui</p> -->
  
−
             <div class="col-lg-12">
+
             <div style="margin-bottom:80px;" class="col-lg-12">
 
             <div class="row">
 
             <div class="row">
  
 
             <div class="col-lg-3" >
 
             <div class="col-lg-3" >
 
             <a data-toggle="tab" data-target="#component1" >
 
             <a data-toggle="tab" data-target="#component1" >
−
             <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+
             <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
 
             src="https://static.igem.org/mediawiki/2017/b/b2/Amazonas_Brazil_Component1.png"
 
             src="https://static.igem.org/mediawiki/2017/b/b2/Amazonas_Brazil_Component1.png"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
Line 222: Line 222:
 
             <div class="col-lg-3" >
 
             <div class="col-lg-3" >
 
             <a data-toggle="tab" data-target="#component2">
 
             <a data-toggle="tab" data-target="#component2">
−
             <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+
             <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
 
             src="https://static.igem.org/mediawiki/2017/4/4b/Amazonas_Brazil_Component2.png"
 
             src="https://static.igem.org/mediawiki/2017/4/4b/Amazonas_Brazil_Component2.png"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
Line 231: Line 231:
 
             <div class="col-lg-3" >
 
             <div class="col-lg-3" >
 
             <a data-toggle="tab" data-target="#component3">
 
             <a data-toggle="tab" data-target="#component3">
−
             <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+
             <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
 
             src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_Components_3.png"
 
             src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_Components_3.png"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
Line 240: Line 240:
 
             <div class="col-lg-3" >
 
             <div class="col-lg-3" >
 
             <a data-toggle="tab" data-target="#component4">
 
             <a data-toggle="tab" data-target="#component4">
−
             <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+
             <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
 
             src="https://static.igem.org/mediawiki/2017/c/ca/Amazonas_Brazil_Components_4.png"
 
             src="https://static.igem.org/mediawiki/2017/c/ca/Amazonas_Brazil_Components_4.png"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
 
             onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
Line 251: Line 251:
 
             <div class="tab-content" align="center">
 
             <div class="tab-content" align="center">
 
               <div id="component1" class="team-content tab-pane fade" >
 
               <div id="component1" class="team-content tab-pane fade" >
−
               <p>Cas9 (BBa_K2457001):
+
               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/a6/Amazonas_Brazil_Cas9.png">
−
                The standardized Cas9 device under control of AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair pathways, it will lay the road to genomic editing in living cells.
+
−
              </p>
+
 
               </div>
 
               </div>
 
               <div id="component2" class="team-content tab-pane fade" >
 
               <div id="component2" class="team-content tab-pane fade" >
−
               <p>guide RNA (BBa_K2457002):
+
               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/6/63/Amazonas_Brazil_sgRNA.png">
−
                This biobrick is constitutively expressed by J23100 promoter and codify an optimized sgRNA chimera composed of a protospacer crRNA with a GG motif at the 3’ end of its target-specific sequence, a tracrRNA with extended duplex length and a mutation at the thymine 4. It was rationally engineered to improve the editing efficiency of CRISPR-Cas9 machinery. When expressed, it guides the Cas9 nuclease activity through Rosalind-Watson-Crick base pairing complementarity with the target sequence..
+
            <img class="img-responsive" src="https://static.igem.org/mediawiki/parts/b/b8/SgRNA_ESTRUTURA.png">
−
              </p>
+
 
               </div>
 
               </div>
 
               <div id="component3" class="team-content tab-pane fade" >
 
               <div id="component3" class="team-content tab-pane fade" >
−
               <p>RecA (BBa_K2457003)
+
               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/24/RECA.png">
−
              RecA is an essential building block part which leads the homologous recombination AND DNA insertion into the genome. After the target sequence cleavage catalyzed by the Cas9-sgRNA complex, the SOS response machinery is activated. As an output of the repair pathway, RecA builds a DNA-protein filament which investigates for a homologous template on undamaged DNA sequences, assisting in the invasion process and setting-up the Holliday Junction. This generates a crossover with the strands, leading to the recombination among the undamaged strand and the broken strand.
+
−
              </p>
+
 
               </div>
 
               </div>
 
               <div id="component4" class="team-content tab-pane fade" >
 
               <div id="component4" class="team-content tab-pane fade" >
−
               <p>Donor DNA (Bba_K2457004)
+
               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_DonorDNA.png">
−
                This BioBrick contains homologous arms (300bp upstream and downstream from the Operon Lac, to increase the efficiency) as a template to insert information into genome on the repair pathway of cells. Allows the recombination through the RecA activity by Homology Directed Repair (HDR) pathway. It has in its coding sequence the Green Fluorescent Protein (GFP) gene which will operate as a reporter to recombinant cells that will become fluorescents.
+
−
              </p>
+
 
               </div>
 
               </div>
 
             </div>
 
             </div>
Line 283: Line 276:
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
               src="https://static.igem.org/mediawiki/2017/5/5a/Amazonas_Brazil_Project_8_2.png">
 
               src="https://static.igem.org/mediawiki/2017/5/5a/Amazonas_Brazil_Project_8_2.png">
 +
 +
            <img class="img-responsive" alt="Erro" style="width: 70%;height: 80%;margin-left: 10%"
 +
              src="https://static.igem.org/mediawiki/2017/d/db/CROUT.png">
  
 
  <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
 
  <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
Line 300: Line 296:
  
 
             </div>
 
             </div>
−
              <h2 style="margin-top: 1.4em;text-align: center;">Design</h2>
+
           
−
              <h3 class="SubTitle">Check all the parts involved and our approaches with closer details!</h3>
+
−
              <p class="p">
+
−
                <h3 class="SubTitle">Cas9 (BBa_K2457001)</h3>
+
−
                <p class="p">The standardized Cas9 device under control of AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to
+
−
                  double-strand breaks. Through cell repair pathways, it will lay the road to genomic editing in living cells. </p>
+
−
 
+
−
                <h3 class="SubTitle">Optimized sgRNA (BBa_K2457002)</h3>
+
−
                <p class="p">This biobrick is constitutively expressed by J23100 promoter and codify an optimized sgRNA chimera composed of a protospacer crRNA with a GG motif at the 3’ end of its target-specific sequence, a tracrRNA with extended duplex length and
+
−
                  a mutation at the thymine 4. It was rationally engineered to improve the editing efficiency of CRISPR-Cas9 machinery. When expressed, it guides the Cas9 nuclease activity through Rosalind-Watson-Crick base pairing complementarity with
+
−
                  the target sequence. </p>
+
−
 
+
−
                <h3 class="SubTitle">RecA (Bba_K2457003)</h3>
+
−
                <p class="p">RecA is an essential building block part which leads the homologous recombination AND DNA insertion into the genome. After the target sequence cleavage catalyzed by the Cas9-sgRNA complex, the SOS response machinery is activated. As an
+
−
                  output of the repair pathway, RecA builds a DNA-protein filament which investigates for a homologous template on undamaged DNA sequences, assisting in the invasion process and setting-up the Holliday Junction. This generates a crossover
+
−
                  with the strands, leading to the recombination among the undamaged strand and the broken strand. </p>
+
−
 
+
−
                DONOR DNA
+
  
 
               </p>
 
               </p>
Line 339: Line 318:
  
 
<footer style="position:fixed;">
 
<footer style="position:fixed;">
−
<div class="footer-container" >
+
<div class="subcontainer">
−
<a href="#TOPPER"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="witdh:100%"></a>
+
 
 +
<a href="#"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="width: 4%;position: absolute;bottom: 0.5em;right: 0.5em;opacity: 0.5;"></a>
 
</div>
 
</div>
 
</footer>
 
</footer>
Line 356: Line 336:
 
         <i class="fa fa-facebook" aria-hidden="true"></i>
 
         <i class="fa fa-facebook" aria-hidden="true"></i>
 
     </a>
 
     </a>
 +
    <a href="#"><img src="https://static.igem.org/mediawiki/2017/5/55/T--Amazonas_Brazil--IconTop.png" style="width: 4%;position: absolute;bottom: 5.5em;right: 0.5em;opacity: 0.5;"></a>
 
     </p>
 
     </p>
 
   </div>
 
   </div>

Latest revision as of 03:52, 2 November 2017

Wiki_iGEM_Amazonas

PROJECT

pCRISPeasy

Navigate into CRISPeasy roadmap

Erro Erro Erro Erro