Difference between revisions of "Team:Amazonas Brazil/Project"

Revision as of 02:41, 2 November 2017 (view source)

Isaacguerrreiro (Talk | contribs)

← Older edit

Latest revision as of 03:52, 2 November 2017 (view source)

Isaacguerrreiro (Talk | contribs)

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<~~p>Cas9 (BBa_K2457001)~~:

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~~The standardized Cas9 device under control of AraC_pBAD regulatory module~~. ~~In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to double-strand breaks~~. ~~Through cell repair pathways, it will lay the road to genomic editing in living cells.~~

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</p>

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<~~p>guide RNA (BBa_K2457002):~~

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~~This biobrick is constitutively expressed by J23100 promoter and codify an optimized sgRNA chimera composed of a protospacer crRNA with a GG motif at the 3’ end of its target~~-~~specific sequence, a tracrRNA with extended duplex length and a mutation at the thymine 4~~. It was rationally engineered to improve the editing efficiency of CRISPR-Cas9 machinery. When expressed, it guides the Cas9 nuclease activity through Rosalind-Watson-Crick base pairing complementarity with the target sequence..

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</p>

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</div>

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<~~p>RecA (BBa_K2457003)~~

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~~RecA is an essential building block part which leads the homologous recombination AND DNA insertion into the genome. After the target sequence cleavage catalyzed by the Cas9~~-~~sgRNA complex, the SOS response machinery is activated~~. As an output of the repair pathway, RecA builds a DNA-protein filament which investigates for a homologous template on undamaged DNA sequences, assisting in the invasion process and setting-up the Holliday Junction. ~~This generates a crossover with the strands, leading to the recombination among the undamaged strand and the broken strand.~~

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</p>

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</div>

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<~~p>Donor DNA (Bba_K2457004)~~

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~~This BioBrick contains homologous arms (300bp upstream and downstream from the Operon Lac, to increase the efficiency) as a template to insert information into genome on the repair pathway of cells~~. ~~Allows the recombination through the RecA activity by Homology Directed Repair (HDR) pathway~~. ~~It has in its coding sequence the Green Fluorescent Protein (GFP) gene which will operate as a reporter to recombinant cells that will become fluorescents.~~

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</p>

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</div>

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Difference between revisions of "Team:Amazonas Brazil/Project"

Latest revision as of 03:52, 2 November 2017

PROJECT

pCRISPeasy

Navigate into CRISPeasy roadmap

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               <div class="col-lg-3" >
               <a data-toggle="tab" data-target="#component1" >
-              <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+              <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
               src="https://static.igem.org/mediawiki/2017/b/b2/Amazonas_Brazil_Component1.png"
               onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
@@ Line 222: / Line 222: @@
               <div class="col-lg-3" >
               <a data-toggle="tab" data-target="#component2">
-              <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+              <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
               src="https://static.igem.org/mediawiki/2017/4/4b/Amazonas_Brazil_Component2.png"
               onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
@@ Line 231: / Line 231: @@
               <div class="col-lg-3" >
               <a data-toggle="tab" data-target="#component3">
-              <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+              <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
               src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_Components_3.png"
               onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
@@ Line 240: / Line 240: @@
               <div class="col-lg-3" >
               <a data-toggle="tab" data-target="#component4">
-              <img alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
+              <img class="img-responsive" alt="Erro" style="width: 100%;height: 100%;margin-left: 10%;opacity: 1"
               src="https://static.igem.org/mediawiki/2017/c/ca/Amazonas_Brazil_Components_4.png"
               onmouseover="this.style.opacity=0.5;this.filters.alpha.opacity=100"
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              <div class="tab-content" align="center">
                <div id="component1" class="team-content tab-pane fade" >
-               <p>Cas9 (BBa_K2457001):
+               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/a6/Amazonas_Brazil_Cas9.png">
-                The standardized Cas9 device under control of AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair pathways, it will lay the road to genomic editing in living cells.
-              </p>
                </div>
                <div id="component2" class="team-content tab-pane fade" >
-               <p>guide RNA (BBa_K2457002):
+               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/6/63/Amazonas_Brazil_sgRNA.png">
-                This biobrick is constitutively expressed by J23100 promoter and codify an optimized sgRNA chimera composed of a protospacer crRNA with a GG motif at the 3’ end of its target-specific sequence, a tracrRNA with extended duplex length and a mutation at the thymine 4. It was rationally engineered to improve the editing efficiency of CRISPR-Cas9 machinery. When expressed, it guides the Cas9 nuclease activity through Rosalind-Watson-Crick base pairing complementarity with the target sequence..
+             <img class="img-responsive" src="https://static.igem.org/mediawiki/parts/b/b8/SgRNA_ESTRUTURA.png">
-              </p>
                </div>
                <div id="component3" class="team-content tab-pane fade" >
-               <p>RecA (BBa_K2457003)
+               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/24/RECA.png">
-               RecA is an essential building block part which leads the homologous recombination AND DNA insertion into the genome. After the target sequence cleavage catalyzed by the Cas9-sgRNA complex, the SOS response machinery is activated. As an output of the repair pathway, RecA builds a DNA-protein filament which investigates for a homologous template on undamaged DNA sequences, assisting in the invasion process and setting-up the Holliday Junction. This generates a crossover with the strands, leading to the recombination among the undamaged strand and the broken strand.
-              </p>
                </div>
                <div id="component4" class="team-content tab-pane fade" >
-               <p>Donor DNA (Bba_K2457004)
+               <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/23/Amazonas_Brazil_DonorDNA.png">
-                This BioBrick contains homologous arms (300bp upstream and downstream from the Operon Lac, to increase the efficiency) as a template to insert information into genome on the repair pathway of cells. Allows the recombination through the RecA activity by Homology Directed Repair (HDR) pathway. It has in its coding sequence the Green Fluorescent Protein (GFP) gene which will operate as a reporter to recombinant cells that will become fluorescents.
-              </p>
                </div>
              </div>
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                <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
                src="https://static.igem.org/mediawiki/2017/5/5a/Amazonas_Brazil_Project_8_2.png">
+             <img class="img-responsive" alt="Erro" style="width: 70%;height: 80%;margin-left: 10%"
+              src="https://static.igem.org/mediawiki/2017/d/db/CROUT.png">
   <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"