Difference between revisions of "Team:SZU-China/Parts"

(Prototype team page)
 
 
(27 intermediate revisions by 5 users not shown)
Line 1: Line 1:
−
{{SZU-China}}
 
 
<html>
 
<html>
  
 +
<head>
  
 +
    <style>
 +
        #sideMenu, #top_title {
 +
            display: none;
 +
        }
  
 +
        #content {
 +
            width: 100%;
 +
            margin: 0px;
 +
            padding: 0px;
 +
            background: #FFF;
 +
        }
  
 +
        body {
 +
            background-color: #fff;
 +
        }
  
 +
        .table a:hover {
 +
            color: rgb(75, 151, 165) !important;
 +
        }
  
−
<div class="column full_size">
 
  
−
<h1>Parts</h1>
 
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
        .table a {
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
            font-size: 15px !important;
 +
            font-weight: 600 !important;
 +
        }
  
 +
        .font1 {
 +
            font-size: 17px;
 +
        }
 +
        td{
 +
            font-size:15px
 +
        }
 +
    </style>
  
−
</div>
+
    <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
  
 +
    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:SZU-China/sub/CSS?action=raw&amp;ctype=text/css">
 +
    <link href="https://2017.igem.org/Template:SZU-China/HP/CSS?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
 +
    <link href="https://2017.igem.org/Template:SZU-China/table/CSS?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
  
 +
    <script type="text/javascript" src="https://2017.igem.org/Template:SZU-China/jquery/Javascript?action=raw&amp;ctype=text/javascript"></script>
  
 +
    <script type="text/javascript">
 +
        $(document).ready(function () {
 +
            $("#nav-list-ul li").hover(function () {
 +
                $(this).find('ul.sub-nav').show();
 +
            }, function () {
 +
                $(this).find('ul.sub-nav').hide();
 +
            });
  
 +
            $("div.navbar").hover(function () {
 +
                $(this).css("box-shadow", "1px 1px 7px #000")
 +
            }, function () {
 +
                $(this).css("box-shadow", "0px 0px 0px #000")
 +
            });
  
−
<div class="column half_size">
+
            $("#top").click(function () {
−
<div class="highlight">
+
                $('body,html').animate({ scrollTop: 0 }, 200);
−
<h5>Note</h5>
+
                return false;
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
            });
−
</div>
+
        });
−
</div>
+
    </script>
  
 +
</head>
  
 +
<body>
 +
    <div class="navbar" style="top:5px;position:fixed;z-index:5">
 +
        <div class="navbar-nav">
 +
            <ul id="nav-list-ul" class="nav-list">
  
 +
                <li>
 +
                    <a href="#">TEAM</a>
 +
                    <span class="caret"></span>
 +
                    <ul class="sub-nav" style="top:-5px">
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Team">MEMBERS</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Collaborations">COLLABORATIONS</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Attributions">ATTRIBUTION</a></li>
 +
                    </ul>
 +
                </li>
  
−
<div class="column half_size">
 
  
−
<h5>Adding parts to the registry</h5>
+
                <li>
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
                    <a href="#">PRACTICE</a>
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
                    <span class="caret"></span>
−
</div>
+
                    <ul class="sub-nav" style="top:-5px">
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/HP/Gold_Integrated">INTEGRATED HP</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/HP/Silver">SILVER HP</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Engagement">ENGAGEMENT</a></li>
 +
                    </ul>
 +
                </li>
  
  
 +
                <li>
 +
                    <a href="#">EXPERIMENT</a>
 +
                    <span class="caret"></span>
 +
                    <ul class="sub-nav" style="top:-5px">
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Procedure">PROCEDURE</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Results">RESULTS</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Demonstrate">DEMONSTRATE</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Protocol">PROTOCOL</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Notebook">NOTEBOOK</a></li>
  
 +
                    </ul>
 +
                </li>
 +
                <li>
 +
                    <a href="#">PROJECT</a>
 +
                    <span class="caret"></span>
 +
                    <ul class="sub-nav" style="top:-5px">
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Description">DESCRIPTION</a></li>
  
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Design">DESIGN</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Model">MODEL</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:SZU-China/Safety">SAFTY</a></li>
 +
                    </ul>
 +
                </li>
  
−
<div class="column half_size">
 
  
−
<h5>What information do I need to start putting my parts on the Registry?</h5>
+
                <li>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
                    <a href="#">ACHIEVEMENT</a>
−
<ul>
+
                    <span class="caret"></span>
−
<li>Part Name</li>
+
                    <ul class="sub-nav" style="top:-5px">
−
<li>Part type</li>
+
                        <li><a href="https://2017.igem.org/Team:SZU-China/Achievement">MEDAL</a></li>
−
<li>Creator</li>
+
                        <li><a href="https://2017.igem.org/Team:SZU-China/Parts">PARTS</a></li>
−
<li>Sequence</li>
+
                    </ul>
−
<li>Short Description (60 characters on what the DNA does)</li>
+
                </li>
−
<li>Long Description (Longer description of what the DNA does)</li>
+
            </ul>
−
<li>Design considerations</li>
+
        </div>
−
</ul>
+
        <div>
 +
            <a href="https://2017.igem.org/Team:SZU-China"><img src="https://static.igem.org/mediawiki/2017/1/17/T--SZU-China--team-logo.png" style="height: 36px;width: auto;padding-top: 12px;padding-left:10px;"></a>
 +
        </div>
 +
    </div>
  
−
<p>
 
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
−
</div>
+
    <div style="position: relative;top: 40px;">
  
 +
        <section id="overview" style="padding:106px 0 80px 0;background-color:white;">
 +
            <div class="container">
 +
                <div class="row">
 +
                    <div class="col-sm-12">
 +
                        <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:50px"><center>PARTS</center> </h3>
  
−
<div class="column half_size">
 
  
−
<h5>Inspiration</h5>
+
                    </div>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
                </div>
 +
            </div>
 +
        </section>
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
−
</div>
 
  
−
<div class="column full_size">
+
        <p></p><br /><br />
−
<h5>Part Table </h5>
+
        <section id="overview" class="cd-section" style="padding: 66px 0 50px 0; background-color: rgba(245,245,245,0.45);">
 +
            <div style="position: relative;margin: 30px 14%;">
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
                <p></p>
 +
                <div class="col-sm-12">
 +
                    <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Basic Parts</center> </h3>
 +
                    <span class="font1">This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na<sup>+</sup>-H<sup>+</sup> antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.</span><br /><br />
 +
                    <br/>
 +
                </div>
 +
                <p></p>
 +
                <table class="table" style="width:75%;margin:0 auto">
 +
                    <tbody>
 +
                        <tr>
 +
                            <th style="width:120px">Part Number</th>
 +
                            <th style="width:150px">Name</th>
 +
                            <th style="width:80px">Type</th>
  
−
<div class="highlight">
+
                            <th style="width:300px">Part Description</th>
  
  
−
</html>
+
                        </tr>
−
<groupparts>iGEM17 SZU-China</groupparts>
+
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232000">BBa_K2232000</a></td>
 +
                            <td>TSLV1-CA</td>
 +
                            <td>Basic</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Carbonic Anhydrase α from<i> Bacillus Subtilus TSLV1 </i></td>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232005">BBa_K2232005</a></td>
 +
                            <td>OF4-CA</td>
 +
                            <td>Basic</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Carbonic Anhydrase α from<i> Bacillus Subtilus OF4 </i></td>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232001">BBa_K2232001</a></td>
 +
                            <td>OF4-nhaC</td>
 +
                            <td>Basic</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus OF4 </i></td>
 +
 
 +
                        </tr>
 +
 
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232002">BBa_K2232002</a></td>
 +
                            <td>C125-nhaC</td>
 +
                            <td>Basic</td>
 +
 
 +
                            <td>Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus C125</i></td>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232007">BBa_K2232007</a></td>
 +
                            <td>BS168-nhaC</td>
 +
                            <td>Basic</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus 168 </i></td>
 +
 
 +
                        </tr>
 +
 
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232003">BBa_K2232003</a></td>
 +
                            <td>C125-tupA</td>
 +
                            <td>Basic</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding sequence for another Na<sup>+</sup> - H<sup>+</sup> antiporter TupA from<i> Bacillus Subtilus C125 </i></td>
 +
 
 +
                        </tr>
 +
                       
 +
 
 +
 
 +
                    </tbody>
 +
                </table>
 +
 
 +
                <br /><br />
 +
                <p></p><p></p>
 +
                <span class="font1"></span>
 +
                <span></span><br />
 +
                <span></span><br />
 +
                <span></span><br />
 +
            </div>
 +
 
 +
            <div style="position: relative;margin: 30px 14%;">
 +
 
 +
                <p></p>
 +
                <div class="col-sm-12">
 +
                    <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Composite Parts</center> </h3>
 +
                    <span class="font1">The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.</span><br /><br />
 +
 
 +
                </div>
 +
                <p></p>
 +
                <br />
 +
                <table class="table" style="width:75%;margin:0 auto">
 +
                    <tbody>
 +
                        <tr>
 +
                            <th style="width:120px">Part Number</th>
 +
                            <th style="width:150px">Name</th>
 +
                            <th style="width:80px">Type</th>
 +
 
 +
                            <th style="width:300px;padding-left:5px">Part Description</th>
 +
 
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232011">BBa_K2232011</a></td>
 +
                            <td>RBS-C125-tupA-T1</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125</td>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232012">BBa_K2232012</a></td>
 +
                            <td>P43-OF4-nhaC-T1</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">The gene nhaC from the Bacillus pseudofirmus OF4 encodes the Na+/H+ antiporter</td>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232013">BBa_K2232013</a></td>
 +
                            <td>P43-nhaC-T1</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">This part is the Na+/H+ antiporter coding sequence (CDS) from the B. subtilis strain 168</td>
 +
 
 +
                        </tr>
 +
 
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232014">BBa_K2232014</a></td>
 +
                            <td>P43-TSLV1-CA</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding region of the pink mutant version of amilCP</td>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232015">BBa_K2232015</a></td>
 +
                            <td>P43-OF4-CA</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding region of the purple mutant version of amilCP</td>
 +
 
 +
                        </tr>
 +
 
 +
                        <tr>
 +
                            <td><a href="http://parts.igem.org/Part:BBa_K2232026">BBa_K2232026</a></td>
 +
                            <td>P43-CA-β</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="width:300px;padding-left:5px">Coding region of the green mutant version of amilCP</td>
 +
 
 +
                        </tr>
 +
                    </tbody>
 +
                </table>
 +
 
 +
 
 +
 
 +
 
 +
                <br /><br />
 +
                <p></p><p></p>
 +
                <span class="font1"></span>
 +
                <span></span><br />
 +
                <span></span><br />
 +
                <span></span><br />
 +
            </div>
 +
            <div style="position: relative;margin: 30px 14%;">
 +
                <div class="col-sm-12">
 +
                    <h3 class="uppercase color-primary mb40 " style="margin-bottom: 40px;font-size:28px;font-weight:500"><center>Improved Parts</center> </h3>
 +
 
 +
                </div>
 +
                <span class="font1">This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (<span style="color:#808080">in grey</span>) are presented below. You can click on the part number to see the details.</span><br /><br />
 +
 
 +
                <p></p><br/>
 +
                <table class="table" style="width:75%;margin:0 auto">
 +
                    <tbody>
 +
                        <tr>
 +
                            <th style="width:120px;">Part Number</th>
 +
                            <th style="width:120px">Name</th>
 +
                            <th style="width:85px">Type</th>
 +
 
 +
                            <th style="width:200px">Part Description</th>
 +
                            <th style="width:150px">Improvement</th>
 +
 
 +
                        </tr>
 +
                        <tr>
 +
                            <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232020">BBa_K2232020</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K911008" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K911008</a></td>
 +
                            <td>PsspB-gerAa</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="padding-left:5px">Germination-promoting  receptor (gerA) expression system</td>
 +
                            <td>Optimize its sequence</td>
 +
                        </tr>
 +
                       
 +
                        <tr>
 +
                            <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232022">BBa_K2232022</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1465205" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K2465205</a></td>
 +
                            <td>RBS-CsoS3-T1</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="padding-left:5px">Carbonic anhydrase (csoS3) expression system</td>
 +
                            <td>Optimize its sequence</td>
 +
                        </tr>
 +
                        <tr>
 +
                            <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232025">BBa_K2232025</a><br /><a href="http://parts.igem.org/Part:BBa_K302035" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K302035</a></td>
 +
                            <td>Sucrose-limitation induced kill switch</td>
 +
                            <td>Composite</td>
 +
 
 +
                            <td style="padding-left:5px">Coding region of Sucrose-limitation induced kill switch</td>
 +
                            <td>Characterize in <i>Bacillus Subtilus</i></td>
 +
                        </tr>
 +
                    </tbody>
 +
Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
 +
                </table>
 +
 
 +
            </div>
 +
        </section>
  
−
<html>
 
−
</div>
 
 
</div>
 
</div>
 +
 +
    <div class="arrow-div">
 +
        <a id="top" href="#" title="Back to top">
 +
            <img src="https://static.igem.org/mediawiki/2017/f/f6/T--SZU-China--arrow.png" class="arrow-img">
 +
        </a>
 +
    </div>
 +
 +
</body>
  
  

Latest revision as of 04:01, 2 November 2017

PARTS



Basic Parts

This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na+-H+ antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.


Part Number Name Type Part Description
BBa_K2232000 TSLV1-CA Basic Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus TSLV1
BBa_K2232005 OF4-CA Basic Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus OF4
BBa_K2232001 OF4-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus OF4
BBa_K2232002 C125-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus C125
BBa_K2232007 BS168-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus 168
BBa_K2232003 C125-tupA Basic Coding sequence for another Na+ - H+ antiporter TupA from Bacillus Subtilus C125





Composite Parts

The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.


Part Number Name Type Part Description
BBa_K2232011 RBS-C125-tupA-T1 Composite Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
BBa_K2232012 P43-OF4-nhaC-T1 Composite The gene nhaC from the Bacillus pseudofirmus OF4 encodes the Na+/H+ antiporter
BBa_K2232013 P43-nhaC-T1 Composite This part is the Na+/H+ antiporter coding sequence (CDS) from the B. subtilis strain 168
BBa_K2232014 P43-TSLV1-CA Composite Coding region of the pink mutant version of amilCP
BBa_K2232015 P43-OF4-CA Composite Coding region of the purple mutant version of amilCP
BBa_K2232026 P43-CA-β Composite Coding region of the green mutant version of amilCP





Improved Parts

This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (in grey) are presented below. You can click on the part number to see the details.


Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
Part Number Name Type Part Description Improvement
BBa_K2232020
BBa_K911008		 PsspB-gerAa Composite Germination-promoting receptor (gerA) expression system Optimize its sequence
BBa_K2232022
BBa_K2465205		 RBS-CsoS3-T1 Composite Carbonic anhydrase (csoS3) expression system Optimize its sequence
BBa_K2232025
BBa_K302035		 Sucrose-limitation induced kill switch Composite Coding region of Sucrose-limitation induced kill switch Characterize in Bacillus Subtilus