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<p style="font-family: quicksand;font-size:150%;">RNA thermodynamic Modelling</p> | <p style="font-family: quicksand;font-size:150%;">RNA thermodynamic Modelling</p> | ||
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+ | <center><img src="https://static.igem.org/mediawiki/2017/9/90/Toehold_Switch_Model.jpeg" width="100%" height="auto" class=" igem-logo"></center> | ||
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<p style="font-family: roboto;font-size:125%;color:blue"><b>Assumption 1: Switch MFE (Minimum Free Energy) correlates with the expression leakage</p></b> | <p style="font-family: roboto;font-size:125%;color:blue"><b>Assumption 1: Switch MFE (Minimum Free Energy) correlates with the expression leakage</p></b> | ||
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● The toehold domain (first 15 nts) of the switch RNA is crucial for the binding of the trigger RNA to the switch RNA to initiate the switch-unwinding process: | ● The toehold domain (first 15 nts) of the switch RNA is crucial for the binding of the trigger RNA to the switch RNA to initiate the switch-unwinding process: | ||
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Toehold domain must have minimal paired bases in the switch RNA to ensure the successful binding of this domain with the complementary sequence in the trigger RNA, which allows the unwinding of the switch RNA and permits translation of the reporter protein, RFP, to occur. </p> | Toehold domain must have minimal paired bases in the switch RNA to ensure the successful binding of this domain with the complementary sequence in the trigger RNA, which allows the unwinding of the switch RNA and permits translation of the reporter protein, RFP, to occur. </p> |
Latest revision as of 04:01, 2 November 2017